International Science Community Association Research Journal of Recent Sciences 2277 - 2502 1 10 2012 October 2 Determination of Diafenthiuron Residue in Orange pulp using a matrix Solid-Phase Dispersion Method Coupled to High-Performance Liquid Chromatography with Ultraviolet Detection 55 58 EN Espinoza E. 1 Instituto de Investigaciones Metalurgicas, Universidad Michoacana de San Nicolas de Hidalgo, Ciudad Universitaria Edificio ”U”, Apartado Escudero R. 2 postal 888, C.P. 58000, Morelia, Michoacán, MÉXICO Tavera F.J. 3 PranavC. Atre 1 School of Mechanical and Building Sciences, VIT University, Vellore-632014, Tamilnadu, INDIA ThundilKaruppaRaj R. 2 Mehrdad Zarenejad 1 Department of Economic, Qaemshahr Branch, Islamic Azad University, Qaemshahr, IRAN Rodrigues L.L.R. 1 Department of Mechanical and Manufacturing Engineering, Manipal Institute of Technology, Manipal 576 104, Karnataka, INDIA Kantharaj A.N. 2 Department of Automobile and Aeronautical Engineering, Manipal Institute of Technology, Manipal 576 104, Karnataka, INDIA Kantharaj B. 3 Freitas W.R.C. 4 Murthy B.R.N. 5 Serban C. 1 Faculty of Marketing, The Bucharest University of Economic Studies, Bucharest, ROMANIA Iconaru C. 2 Macovei O.I. 3 Perju A. 4 Kumar Anil 1 Department of Physics, P.L.J.L. Rastogi Inter College, Moradabad 244 001, Uttar Pradesh, INDIA ChauhanRekhaRan RekhaRani 2 Department of Botany, K.G.K. College, Moradabad-244001, Uttar Pradesh, INDIA Kumar Pradeep 3 Department of Physics, K G K College, Moradabad 244001, Uttar Pradesh, INDIA S. Anbarasu 1 Department of Physics, St. Xavier’s College (Autonomous), Palayamkottai – 627002, INDIA DevarajanPrem Anand 2 SheikhKashif Raffat 1 Department of CS and IT, Federal Urdu University of Arts, Sciences and Technology, Karachi, PAKISTAN Mohd.Shahab Siddiqui 2 HIIT, FEST, Hamdard University, Karachi, PAKISTAN Mohd. Siddiq 3 National University of Computer and Emerging Sciences, Karachi, PAKISTAN Shaikh ZubairA. 4 FEST, Hamdard University, Karachi, PAKISTAN AbdulRahman Memon 5 Dubey SharmaRuchi 1 Department of Chemistry BUIT, Barkatullah University Bhopal, MP, INDIA Joshi Smita 2 Department of Chemistry, Sarojini Naidu Govt. Girls PG College, Bhopal, MP, INDIA Amlathe Sulbha 3 Department of Chemistry BUIT, Barkatullah University, Bhopal, MP, INDIA NageswaraRao. T. 1 Department of Engineering Chemistry, Andhra University, Visakhapatnam, AP, INDIA Raghubabu K. 2 SV University, Tirupathi, AP, INDIA D. Sreenivasulu 3 Department of Engineering Chemistry, GITAM University, Hyderabad, AP, INDIA Patrudu T.B. 4 > Research Paper 2012 8 6 2012 October 2 A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of diafenthiuron in orange pulp. The evaluated parameters included the type and amount of sorbent (silica gel and celite) and the nature of eluent (n-hexane, Acetonitrile and saturated aqueous sodium chloride solution). The best results were obtained using 1.0 g of orange pulp sample, 1.0 g of silica gel as sorbet and 20 mL of n-hexane - acetonitrile - saturated aqueous sodium chloride solution (1:1:1), (v/v)). The method was validated using orange pulp samples spiked with diafenthiuron at different concentration levels (0.03 and 0.3 µg/mL). Average recoveries (using each concentration six replicates) ranged 88-94%, with relative standard deviations less than 3%, calibration solutions concentration in the range 0.01-2.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 µg/mL and 0.03 µg/mL respectively. Copyright@ International Science Community Association