Research Journal of Recent Sciences _________________________________________________ ISSN 2277-2502 Vol. 4(ISC-2014), 250-254 (2015) Res. J. Recent. Sci. International Science Congress Association 250 Oxidation of essential oil of Chloroxylonswietenia (Roxb. corom) Telang T.Department of Chemistry and Applied Sciences, Career Point University, Kota, INDIAAvailable online at: www.isca.in, www.isca.me Received 29th Novmeber 2014, revised 30th March 2015, accepted 28th April 2015 AbstractHydro distilled oil from the leaves of C. swietenia is of unpleasant odour and cannot be marketed but it has medicinal importance. If oxygen content present in this, is increased, then not only its odour will turn into pleasant fragrance but also its quality will be improved for medicinal purposes. With this intention it was oxidized using HNO, KMnO, KCr and H as oxidizing agent. H2 was found best suited for oxidation. Antimicrobial activity of oxidized essential oil was found better as compared to un oxidized essential oil which proved improvement in its quality after oxidation. Keywords: Hydro distillation, essential oil, antimicrobial.Introduction Chloroxylonswietenia (Roxb. corom) belonging to Rutaceae family is commonly known as Bherul, Bhirra, Ghirya in Hindi and Satinwood in English. It is 9-15 meter high monotypic genus of timber yielding tree found in India and Ceylon. It is widely found in dry deciduous forests in India at an altitude from 1000 to 5000 meter.This plant has medicinal uses. Figure-1 The important studies on the essential oil of this plant have been made but there had been no oxidation study done so far Research Journal of Recent Sciences ______________________________________________________________ ISSN 2277-2502Vol. 4(ISC-2014), 250-254 (2015) Res. J. Recent. Sci. International Science Congress Association 251 The essential oil from the leaves of . sweitenia does not have pleasing odour and cannot be marketed. In the present work attempt is made to improve the odour and the quality of the essential oil by oxidation. Essential oil contain oxygenating molecules which transport the nutrients to the cells of the body. Clinical research shows that essential oils may help to create an environment in which harmful bacteria, virus, fungi etc. cannot survive. Diseases cannot exist in an oxygen rich environment. If oxygenated components in the essential oil are increased, its fragrance will improve and it will not only be more useful for medicines but also be more useful for industrial and cosmetic uses. Material and Methods The fresh leaves of . sweitenia were collected from Pachmari forest in Hoshangabad district of Madhya Pradesh during September to December. The essential oil (0 .91% v/w) obtained from the hydrodistillation of shade dried leaves was analysed for physico-chemical properties. Using various methods. after physicochemical analysis and identifying components essential oil was oxidised. Various oxidants viz. potassium permanganate (KMnO), potassium dichromate (KCr), nitic acid (HNO) and hydrogen peroxide (H) were tried for oxidising the essential oil obtained from the C. Swietenia. The experimental procedure adopted for the study is given below. Experimental: 1.0 ml.of essential oil was taken in a conical flask ( 20ml capacity) and to it 1.00ml of 30 volume hydrogen peroxide was added. The reaction mixture was kept at room temperature ( 28C) for 24 hours. The reaction was studied at different timing i.e. 6 hours, 12 hours, 18 hours and 24 hours. The same procedure was adopted at two different temperatures i.e. 50C and 80C. The observations are recorded in table 1, table 2 and table 3. A similar procedure was adopted to study the oxidation of the essential oil using potassium permanganate, potassium dichromate and nitric acid at three temperatures i.e 28C, 50C and 80C. The quantity of each oxidant was taken as 0.01 mol/100ml. volume. The observations are recorded in table 4-12. Table-1 Oxidant: Hydrogen peroxide (H). Temperature: 28C S.N. Time inHours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4 6 12 18 24 Yellow Yellow Faint Yellow Faint Yellow No change in fragrance Change in fragrance Fragrance increased No change in fragrance Table-2 Oxidant: Hydrogen peroxide (H). Temperature: 50C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Yellow Faint Yellow Faint Yellow Faint Yellow Agreeable change in fragrance Fragrance increased significantly No change in fragrance No change in fragrance Table-3 Oxidant: Hydrogen peroxide (H). Temperature: 80C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Faint Yellow Faint Yellow Faint Yellow Faint Yellow Agreeable change in fragrance Fragrance increased significantly No change in fragrance No change in fragrance Table-4 Oxidant: Potassium permanganate (KMnO). Temperature: 28C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Pink Pink Pink Pink Fragrance increased ( not agreeable ) No change in fragrance No change in fragrance No change in fragrance Research Journal of Recent Sciences ______________________________________________________________ ISSN 2277-2502Vol. 4(ISC-2014), 250-254 (2015) Res. J. Recent. Sci. International Science Congress Association 252 Table-5 Oxidant: Potassium permanganate (KMnO). Temperature 50C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Pink Pink Faint pink Faint pink No significant change No significant change No significant change No significant change Table-6 Oxidant: Potassium permanganate (KMnO). Temperature: 80C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Pink Faint pink Faint pink Faint pink No change in fragrance No change in fragrance No change in fragrance No change in fragrance Table-7 Oxidant: Potassium dichromate (KCr). Temperature: 28C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Yellow Yellow Yellow Faint yellow No Change in fragrance hange in fragrance hange in fragrance No change in fragrance Table-8 Oxidant: Potassium dichromate (KCr). Temperature: 50C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Yellow Faint green Faint green Faint green No change in fragrance Slight change in fragrance No change in fragrance No change in fragrance Table-9 Oxidant: Potassium dichromate (KCr). Temperature: 80C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Faint green Faint green Faint green No change In colour Not significant Not significant No change in fragrance No change in fragrance Table-10 Oxidant: Nitric Acid (HNO). Temperature: 28C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Pale yellow Pale yellow Pale yellow Pale yellow No change in fragrance No change in fragrance No change in fragrance No change in fragrance Table-11 Oxidant: Nitric Acid (HNO). Temperature: 50C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Pale yellow Pale yellow Pale yellow Pale yellow No change in fragrance No significant fragrance No significant fragrance No change in fragrance Research Journal of Recent Sciences ______________________________________________________________ ISSN 2277-2502Vol. 4(ISC-2014), 250-254 (2015) Res. J. Recent. Sci. International Science Congress Association 253 Table-12 Oxidant: Nitric Acid (HNO). Temperature: 80C S.N. Time in Hours Colour of Essential Oil Odour of Essential Oil 1. 2. 3. 4. 6 12 18 24 Pale yellow Pale yellow Pale yellow Pale yellow No significant fragrance No significant fragrance No significant fragrance No significant fragrance Determination of amount of hydrogen peroxide H used for the oxidation of 1.0 ml of essential oil of chloroxylon swieteniaExperimental: 1.0 ml of essential oil of C. swietenia was taken in to a 50ml conical flask and 5.0ml volume hydrogen peroxide (H) was to added it. This mixture was kept at 50C temperature. After 12 hours 10ml. of potassium iodide (KI, 10%) solution were added to the mixture. The mixture was titrated against, standard hypo (Na) (N/20) solution using starch indicator. Same procedure was repeated with 5.0 ml of 30 volume H2 without essential oil (blank titration). Table-13 Observation Table of Volume of Hypo used for the reaction with Hydrogen peroxide (Without Essential oil) [Blank] Volume of Hydrogen peroxide Volume of Hypo Initial Volume (a) Final Volume (b) Volume of Hypo used ( b-a) 5.0 ml. 5.0 ml. 5.0 ml. 0.0 ml. 0.0 ml. 0.0 ml. 11.3. ml. 11.2. ml. 11.2 ml 11.3. ml. 11.2. ml. 11.2 ml Volume of Hypo used for the titration with 5.0 ml. Of H(Blank) = 11.2 ml. Table-14 Observation Table of Volume of Hypo used for the reaction with Hydrogen peroxide (With 1 ml Essential oil) S.N. Volume of Hydrogen Peroxide Volume of Hypo Initial Volume (a) Final Volume (b) Volume of Hypo used ( b-a) 1. 2. 3. 5.0 ml. 5.0 ml. 5.0 ml. 0.0 ml. 0.0 ml. 0.0 ml. 9.7 ml. 9.5 ml. 9.5 ml 9.7. ml. 9.5. ml. 9.5 ml Volume of Hypo used for the titration with 5.0 ml. Of H(with 1.0 ml. Essential oil) = 9.5 ml. On calculating the volume and amount of hydrogen peroxide used for the oxidation 1.0.ml. of essential oil of C.swieteniawere found to be 0.76 ml. nd 0.003 gm respectively from the observation TABLE 13 and 14. The unoxidised and oxidised essential oils were analysed for physico chemical constants and constituents present in them. To analyse components present in the oxidised essential oil, Thin layer Chromatography, Column Chromatography, Gas liquid Chromatography, Gas Chromatography, Mass-Spectroscopy and Infra Red Spectroscopy techniques were used. Components present in both the essential oils were compared, which is tabulated as under in table 15. Table-15 Components of Unoxidised and Oxidised Essential Oils: A Comparision Component Unoxidised essential oil (Concentration of component) %Oxidised essential oil (Concentration of component) % - pinene Camphene Limonene -Pinene 3 Carene Myrcene -Phellandrene P-Cymeme -Terpinene -Terpineol Methyl heptenone Citral-a Citral-b Geraniol Linalool -Caryophyllene oxide Nerol Geranyl acetate - Caryophyllene - Caryophyllene Methyl cinnamate -cadinene 0.11 0.78 2.78 0.08 3.17 0.83 0.10 0.93 9.29 12.5 12.29 4.05 2.22 1.05 1.75 18.40 6.54 3.34 5.40 2.83 3.22 6.20 0.40 0.42 28.95 Nil 1.94 Nil 1.76 Nil 4.30 4.34 Nil Nil Nil 1.97 0.42 47.73 Nil 0.22 Nil Nil 0.07 Nil Three new components, Copaene, Hexahydrodimethyl naphthalene and Cyclobuta 1,2,3,4, dicyclopentene were reported by GC-MS analysis of oxidised oil of . swietenia.The change in components and their percentages of concentration were due to possibility of isomerisation, re-arrangement elimination, addition and substitution reactions. Research Journal of Recent Sciences ______________________________________________________________ ISSN 2277-2502Vol. 4(ISC-2014), 250-254 (2015) Res. J. Recent. Sci. International Science Congress Association 254 Oxidised essential oil was screened for its antimicrobial activity against four gram positive bacteria and sixteen gram-negative bacteria using paper disc agar diffusion method5 and against thirteen fungi. In the light of above observations it may be suggested that oxidised essential oil of C. swietenia may be used as antifungal and antibacterial agent against some bacteria viz. Shigella shiga, Sh. flexneri. Vibrio cholerae Ogawa, Bacillus mycoides, B. pumilus and Vibrio cholerae Ianwa. The activity of some fungiviz. Aspergillus oryzae, A. terreus, Curvularia prasadii, Candida albicans and Trichoderma viride also can be suppressed using this if the in vitro studies hold good under in vivo conditions. Results and Discussion The essential oil of . sweitenia was oxidised using H. The odour of the oil was slightly improved and became agreeable. The time and temperature required for oxidation were found to be 12 hrs. and 50C respectively. The amount H used to oxidise 1.0 ml of the essential oil was found to 0.76ml. GLC and GC-MS analysis reported the presence of eighteen components in the oxidised oil out of which three components could not be identified. -caryophyllene oxide was found to be the major components of oxidised oil. The improvement of odour was due to change in the percentages of components after oxidation, which may probably be due to addition elimination and rearrangement reactions and isomerisation of components. The increase in the concentrations of oxidised components i.e. Geraniol and -caryophyllene oxide indicated that the oxygen content had increased after oxidation therefore the odour and quality of oil was improved. Conclusion Oxidised essential oil of C.sweitenia can be used as antibacterial and antifungal agent against some specific bacteria and fungi if the in vitro studies hold good under in vivo conditions and can be marketed with good fragrance and quality for medicinal uses. AcknowledgementThe authors are thankful to the N.C.L. pune for providing the facilities of GLC and GC-MS and Pathological Laboratory, Betul to give the facility for antimicrobial study. We pay gratitude to Govt. N.M.V. Hoshangabad, for giving facilities in the college. References 1.Gerg S.C. and Oswal V.B. In vitro antifungal activity of the essential oil from the leaves of Chloroxylon swietenia, Indian Drugs19(5), 189-91, (1981)2.Gerg S.C. and Oswal V.B., In vitro antibacterial activity of the essential oil from leaves of Chloroxylon swieteniaRoxb. corom, Reichstoffee aromen Kosmetica,32(2), 36-7, (1982)3.Gerg S.C. and Oswal V.B. Essential oil from leaves of Chloroxylon swietenian (Roxb.corom), Indian perfumer 40(3), 76-8, (1996)4.http://members.tripod.com/-LoRee/intro.html. (2014)5.Maruzzella J.C. and Henry P.A., The antifungal activity of perfume oils, J. Amer pharm Ass., 47, 471-76, (1958)