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Estimation of chlorophyll content in local Caulerpa seaweeds using acetone, DMSO and Methanol

Author Affiliations

  • 1Department of Chemistry, De La Salle University - Manila, Philippines
  • 2Department of Chemistry, De La Salle University - Manila, Philippines
  • 3Department of Chemistry, De La Salle University - Manila, Philippines

Res.J.chem.sci., Volume 8, Issue (10), Pages 1-5, October,18 (2018)


Being photosynthetic organisms, seaweeds are comprised of different pigments which give rise to their coloration and thus classification into either being brown (Phaeophyceae), green (Chlorophyceae), or red (Rhodophyceae) seaweeds. Over the years, research regarding macroalgal pigments has grown due to the biological activities of these pigments. Thus, several methods have been developed for the estimation of macroalgal pigments. This study aims to conduct an estimation of pigment specifically the chlorophyll content from local Caulerpa seaweeds using acetone, DMSO, and methanol as solvents for extraction, to compare the amount of pigments present in Caulerpa seaweeds, as well as compare the extraction efficiency of the solvents. Fresh seaweeds were purchased from local markets in the Philippines during October 2016. The pigments were extracted using the three solvents and extracts were analyzed using UV-VIS spectrophotometry at wavelength maxima of the pigments. Estimation of chlorophyll content was made using the methods of Porra (1989), and Barnes et al. (1991). Comparisons were made between the chlorophyll content obtained from the different seaweed species with regard to the solvent of extraction. Results of the study indicate variations in chlorophyll content of the different Caulerpa seaweeds caused by the solvents used for extraction.


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