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Risks of Urinary Infection and Exposure to Nitrites through Consumption of Water in SO-AVA

Author Affiliations

  • 1Health and Medical Research Institute, P. O. Box: 974, Abomey-Calavi, Cotonou, BENIN
  • 2 Laboratory of Applied Research in Biology (LARBA), Environment Engineering Department of Polytechnic University (EPAC), University of Abomey-Calavi (UAC) 01BP 526 Cotonou, BENIN
  • 3 Laboratory of Environmental Health and Toxicology, Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi (UAC), 03 BP 1463, JERICHO
  • 4 Sciences of Earth Department, Faculty of Sciences and Techniques, University of Abomey-Calavi (UAC), 01BP 526 Cotonou, BENIN
  • 5 Department of Physiology and Pharmacology, Faculty of Sciences, University of Lom (UL), TOGO
  • 6 Biochemistry and Cellular Biology, University of Abomey-Calavi (UAC), 01BP 526 Cotonou, BENIN

Res.J.chem.sci., Volume 4, Issue (3), Pages 17-20, March,18 (2014)


Our previous works had shown that drinking water in SO-Ava was contaminated with nitrates and their by-products. These nitrates by-products are extremely toxic for human being because of their capacity to transform hemoglobin in methemoglobin under the effect of Nitrosomonas and Nitrobacter bacteria especially with infants. It is in this context that the present study was conducted in order to examine the toxicity of these compounds on the inhabitants of Ahomey and Houdo to S-Ava, localities the most exposed to these xenobiotics through the drinking water. After urine and blood analyses on empty stomach, the results showed the presence of nitrites in urines in the case of 80 % of investigated followed by leucocyturia and by proteinuria. These symptoms added to the results of the blood parameters are proofs of an exposure with nitrates and an infection of enterobacteriological origin. The populations of these localities are thus exposed to the methemoglobin.


  1. Menard C., Assessment of dietary exposure of nitrate and nitrite in France, Food Additives & Contaminants, (2008)
  2. Agbandji Lucien; Patrick, Edorh A.; Gbago, Georges B.; Koumolou, Luc; Adisso, Sosthene; Guedenon, Patient; Ahodjide, Soulemane; Sinsin, Brice and Boko, Michel, Comparison of Heavy Metals Contents for Some Cigarettes Brands, American Journal of Pharmacology & Toxicology, (2012)
  3. Fan A.M., Willhite C.C. and Book S.A., Evaluation of the nitrate drinking water standard with reference to infant methemoglobinemia and potential reproductive toxicity, Regul Toxicol Pharmacol, 7(2), 135-148 (1987)
  4. L'hirondel J. and L'hirondel J.L., Nitrate and man: toxic, harmless or beneficial,CABI Publishing, 168, (2002)
  5. Gangolli S.D., Nitrate, nitrite and N-nitroso compounds, European Journal of Pharmacology: Environmental Toxicology and Pharmacology, 1101 (1994)
  6. Fan A.M. and Steinberg V.E., Health implications of nitrate and nitrite in drinking water: an update on methemoglobinemia occurrence and reproductive and developmental toxicity, Regul Toxicol Pharmacol, 23, 35-43 (1996)
  7. Wei M., Chemopreventive effects of S- methylcysteine on rat hepatocarcinogenesis induced by concurrent administration of sodium nitrite and morpholine, Cancer Letters, 1208 (2000)
  8. Kinsiclounon G.E., Edorh P.A., Guedenon P., Deguenon Y., Sossou B., Dougnon V.T., Loko F., Boko M.,Risks of Drinking Water Contamination by Chemical and Organic Substances in the Lakeside City of So-Ava in Benin Republic, International Research Journal of Environment Sciences, 2(1), 49-57 (2013)
  9. Clautilde Teugwa Mofor, Effects Of Vitamin A And Iron Supplementation on the Treatment of Malaria In Cameroonian Preschool Children, Internet Journal of Tropical Medicine/15402681, 20060919 (2006)
  10. Loko F., Biochimie analytique-physiopathologie-explorations fonctionnelles, manuel l'usage des tudiants des techniques de biologie humaine, Edition CPU, 120-145 (2001)
  11. Ellen G. and Schuller P.L., Nitrite, origin of continuous ansciety, Londres : Preusman Edition (1983)
  12. Boukerche S., Aouacheri W., Saka S., Effect of shortterm alphamethrine treatment on hepatic glutathione redox system in rats, Arab J Pharm Sci., 3, 25-30 (2007)
  13. Komarov B., In vivo spin trapping of nitric oxide in mice, Biochem Biophys Res Commun;193, 1191-8 (1993)
  14. Fritsch P. and Saint-Blanquat G., Les nitrates et les nitrites, apports alimentaires et leur devenir, Sci Alim;12, 563-576 (1992)
  15. Eisenbrand G. Spregelhalder B. Preussmann R., Nitrite and nitrite in saliva, Oncology, 37, 227-31 (1970)
  16. Vialia A., Elments de toxicologie, Paris: ditions mdicales internationales, 12 (1998)
  17. Savino F. Maccario S. Guidi C., Methemoglobinemia caused by the ingestion of Metab,50, 368-71 (2006)
  18. Turek B., Hlavsova D., Tucek J., Waldman J. and Cerna J., The fate of nitrates and nitrites in the organism, IARC Sci Publ, 31), 625-632 (1980)
  19. Batina P., In vitro kinetics of the oxidative reactivity of nitrate and nitrite in the rat erythrocytes, Food Addit Contam; 1, 145-149 (1990)