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Preparative HPLC for the Purification of major Anthocyanins from Ficus padana burm.L

Author Affiliations

  • 1Department of Chemistry, Andalas University, West Sumatra, INDONESIA

Res.J.chem.sci., Volume 3, Issue (12), Pages 60-64, December,18 (2013)


Anthocyanin can be purified from raw extract of Ficus padana burm.L by applying preparative high performance liquid chromatography (HPLC). For every separation process, the chromatographic condition need to be optimized in order to gain the efficient purification process instantly. The current study shows that analytical scale chromatographic condition can be used as a condition for preparativehplc. Two known acylated anthocyanins are isolated from the Ficus padana burm.L by Step gradient polarity preparative HPLC. The structures are set based on spectrometric analysis. The data from each obtained anthocyanin is being compared with both mass spectral data and published data. The first compound (peak 1) was identify as a pelargonidin 3-(6”-p-coumarylglucoside)-5-(4”’-Malonylglucoside) and the second compound was identify as a pelargonidin 3-(6’’-Malonylglucoside).


  1. Gautier-Hion A., Duplantier J.M., Quris R., Feer, F., Sourd C., Decoux J.P., Dubost G., Emmons L., Erard C., Hecketsweiler P., Fruit characters as a basis of fruit choice and seed dispersal in a tropical forest vertebrate community, Oecologia, 65, 324-337 (1985)
  2. Gamble J., Jaeger S.R. and Harker F.R., Preferences in pear appearance and response to novelty among Australian and New Zealand consumers, Postharvest Biol Technol, 41, 38-47 (2006)
  3. Gould K.S., McKelvie J. and Markham K., Do anthocyanins function as antioxidants in leaves? Imaging of H2O2 in red and green leaves after mechanical injury, Plant Cell Environ, 25, 1261-1269 (2002)
  4. Manetas Y., Why some leaves are anthocyanic and why most anthocyanic leaves are red? Flora, 201, 163-177 (2006)
  5. Steyn W.J., Wand S.J.E., Holcroft D.M. and Jacobs G., Anthocyanins in vegetative tissues: a proposed unified function in photoprotection, New Phytol, 155, 349-361 (2002)
  6. Stintzing F.C. and Carle R., Functional properties of anthocyanins and betalains in plants, food, and in human nutrition, Trends Food Sci. Tech, 15, 19-38 (2004)
  7. Huang W., Shao-ling Zhang., Gai-hua Qin., Le Wenquan., Jun Wu., Isolation and determination of mayor anthocyanin pigments in the pericap of P.Communis L. Cv.’Red Du Comines’ and their assoviation with antioxidant activity, African Journal of Agricultural Research, 7(26), 3772-3780 (2012)
  8. Liu Y., Mukarami N Wang L., Si Zhang., Preparative Hing-Perfoemance Liquid Chromatography.,Preparative High-Performance Liquid Chromatography for the Purification of Natural Acylated Abthocyanins from Red Radish (Raphanus sativus L.)., Journal of Chromatography Science,46.743-746(2008)
  9.,.,.Retrieved on: November 10 (2013)
  10. Kong J., Chia L., Goh N., Chia T., Brouillard R., Analysis and biological activities of anthocyanins. Phytochemistry 64. 923–933 (2003)
  11. Delgado-Vargas, F., Paredes-Lopez, O., Natural Colorants for Food andNutraceutical Uses. CRC Press, Boca Raton, FL, p. 326 (2003)
  12. Oki, T., Masuda, M., Furuta, S., Nishiba, Y., Terahara, N., Suda, I., Involvement of anthocyanins and other phenolic compounds in radical-scavengingactivity of purple fleshed sweet potato cultivars, J. Food Sci. 67 (5).1752–1756 (2002)
  13. Pascual-Teresa, S., Santos-Buelga, C., Rivas-Gonzalo, J.C., LC–MS analysis of anthocyanins from purple corn cob, J. Sci. Food Agric, 82.1003–1006 (2002)
  14. Lapornik B., Prosek M. and Golc Wondra A., Comparison of extracts prepared from plant by-products using different solvents and extraction time, J. Food Eng, 71(2), 214–222 (2005)
  15. Jing P. and Giusti M., Effects of extraction conditions on improving the yield and quality of an anthocyanin-rich purple corn (Zea mays L.) color extract, J. Food Sci, 72(7), C363–C368 (2007)
  16. Fan G., Han Y., Gu Z. and Chen D., Optimizing conditions for anthocyanins extraction from purple sweet potato using response surface methodology (RSM), LWT Food Sci Technol, 41, 155–160 (2008)
  17. Steed L.E. and Truong V.D., Anthocyanin content, antioxidant activity, and selected physical properties of flowable purple-fleshed sweetpotato purees, J.Food Sci, 73(5), S215–S221 (2008)
  18. Rivas-Gonzalo J., Analysis of polyphenols. In Methods in Polyphenols Analysis; Santos-Buelga, C., Williamson, G., Eds.;Royal Society of Chemistry (Athenaeum Press, Ltd.), Cambridge,U.K. 95-98, 338-358 (2003)
  19. Strack D. and Wray V., Anthocyanins. In Methods in Plants Biochemistry; Dey, P. M., Harbone, J. B., Eds.; Academic Press: San Diego, CA, Vol. 1: Plant Phenolics,325- 359 (1989)
  20. Huber U. and Majors R.E., Principle in preparative HPLC., Agilent Technologies 5989-66EN(2007)