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Enrichment of Flavonoids from the Methanolic Extract of Boerhaavia Diffusa Roots by Partitioning Technique

Author Affiliations

  • 1Department of Pharmaceutical Chemistry, Krupanidhi College of Pharmacy, Bangalore-560 034, INDIA
  • 2Department of Pharmaceutical Analysis, Krupanidhi College of Pharmacy, Bangalore-560 034, INDIA

Res.J.chem.sci., Volume 3, Issue (1), Pages 43-47, January,18 (2013)


Flavonoids(Rotenoids) present in Boerhaavia diffusa is known for its different biological activities such as antioxidant, anti-diabetic, anti-stress, anticonvulsant, antibacterial, hypoglycemic, anti-nociceptive, hepato-protective, anti-proliferative and anti-estrogenic, anti-inflammatory. The following work constitutes the enrichment of iso-flavonoids present in the methanolic extract of Boerhaavia diffusa by partition technique using various solvents such as acetone, water, Butanol, ethyl acetate, hexane, chloroform and methanol. Enrichment was determined by Thin Layer Chromatography and HPLC and the presence of flavonoids was confirmed with Shinoda test, lead acetate test and sodium hydroxide test.


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