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Study on Kinetics and mechanism of reaction between 2-phenethyl alcohol and vinyl acetate catalyzed by cross-linked Pseudomonas Cepacia Lipase

Author Affiliations

  • 1Department of Chemistry, Dergaon Kamal Dowerah College, Dergaon, Golaghat-785614, Assam, India

Res.J.chem.sci., Volume 12, Issue (2), Pages 32-40, June,18 (2022)


The use of flavoring compounds in the food industry is now increasing day by day. The aim of the present study was to crystallization and cross-linking of lipase from Pseudomonas Cepacia and to study the kinetics of the synthesis of a rose flavor ester, 2-phenethyl acetate in an organic solvent system. The effect of different reaction co-ordinates such as enzyme concentration, temperature, substrate concentration were studied and found the maximum conversion in 50mg/ml enzyme concentration, temperature at 550C and substrate ratio of 1:4. The stability of the cross-linked lipase was observed in comparison to free lipase and retained 50% of original activity by cross linked lipase up to seven days of incubation.


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