International Science Community Association Research Journal of Chemical Sciences 2231 - 606X 1 2 2011 May 18 Spectrophotometric Determination of Ibutilide Fumarate in Bulk Drug substance form 123 127 EN Manocha N. 1 Swami Vivekanand college of Pharmacy, Indore, INDIA Chandra S.K. 2 Suresh Gyan Vihar University, Jaipur, INDIA Sharma V. 3 B.M College of Pharmaceutical Sciences, Indore, INDIA Sangameswaran B. 4 Saluja M. 5 Sathyaraj A. 1 Swami Vivekanand college of Pharmacy, Indore, INDIA Satyanarayana V. 2 Suresh Gyan Vihar University, Jaipur, INDIA Rao M.V.Basaveswara 3 B.M College of Pharmaceutical Sciences, Indore, INDIA Pruthviraj R.D. 1 Dept. of Chemistry, Amruta Institute of Eng. and Mangt. Sci., Bidadi, Bangalore, INDIA Rao G.V. 1 CavinKare Research Centre, 12, Poonamalle Road, Ekkattuthangal, Chennai-600 032, INDIA Annamalai T. 2 Mukhopadhyay T. 3 Madhavi S 4 Machavolu Lakshmi 5 Mbabazi Jolocam 1 Department of Chemistry, Makerere University, P.O. Box 7062, Kampala, UGANDA Ntale M. 2 Department of Chemistry, Kyambogo University, P.O. Box 1, Kyambogo, UGANDA Kwetegyeka J. 3 Department of Chemistry, Gulu University, P.O. Box 166, Gulu, UGANDA Mulongo G. 4 Twinomuhwezi H 5 Nnamuyomba P. 6 Benarjiand K.V. PatruduT. 1 Dept. of Chemistry, College of Eng., GITAM University, Hyderabad campus, Visakhapatnam, INDIA SangerS. H. 1 Dept. of Elec. and other Energy Sources, College of Agri. Eng. and Tech. DBSKKV, INDIA Mohod A.G. 2 Y.P. Khandetode 3 H.Y. Shrirame 4 Deshmukh A.S. 5 hivaraju P.H 1 Dept. of Environmental Science, Yuvaraja Collage, University of Mysore, Mysore, INDIA Olarinoye I.O. 1 Department of Physics, Federal University of Technology, Minna, NIGERIA Iorfa AdamuChristopher 1 Department of Geology, University of Calabar. P.M.B 1115, Calabar,NIGERIA Ntonzi NganjeTherese 2 Dept. of Chemistry, Federal University of Petroleum Resources, Effurum, Delta State, NIGERIA Ukwang E.E. 3 Department of Chemistry, Cross River State University of Technology, NIGERIA Abara IBE Keneth 4 Peter Neji 5 Mbabazi Jolocam 1 Department of Chemistry, Makerere University, P. O. Box 7062, Kampala, UGANDA Solomon Yiga 2 Ssekaalo Henry 3 Sekkina MorsyM.Abou 1 Chemistry Department, Faculty of Science, Tanta University, Tanta, EGYPT Salahuddin NehalA. 2 Ceramic Department, National Research center,Cairo, EGYPT. Elmaghraby A. 3 El-Ashry MonaY. 4 Chakrabarti S.K. 1 Dept. of Physics, M.M.A.M. Campus, Tribhuvan University, Biratnagar, NEPAL Yadav R.P. 2 Dept. of Chemistry, M.M.A.M. Campus, Tribhuvan University, Biratnagar, NEPAL Yadav R.N. 3 Dept. of Mathematics, M.M.A.M. Campus, Tribhuvan University, Biratnagar, NEPAL Reddy Venkateswara V. 1 JNTUH College of Engineering, Hyderabad, INDIA Gnaneswar Kontham 2 Irrigation and CAD Department,TBP HLC Circle, Anantapur , INDIA Venkata RamanaNelluru 3 JNTUA College of Engineering, Anantapur, INDIA Sashidhar Chundupalli 4 Das Diamond 1 Department of Chemical Engineering, National Institute of Technology, Rourkela, Orissa, INDIA dandapat Jyotsna Snigdha 2 Department of Biotechnology, FM University, Balasore, INDIA Bhattarai Jagadeesh 1 Central Department of Chemistry, Tribhuvan University, Kathmandu, NEPAL Xiao-hua CAI 1 College of Chemistry and environmental Science, Guizhou University for Nationalites, Guiyang, CHINA XIE Bing 2 Babu M.Satya 1 SAP Limited, Hyderabad, INDIA Soni Nitisha 2 Srinivas B.V. 3 > Research Paper 2011 1 16 2011 May 18 A simple and cost effective spectrophotometric method is described for the determination of Ibutilide fumarate in bulk drug substance form. The method is based on ‘complex formation’ concept under acidic conditions with acid dyes e.g. TPOOO. The colored species have absorption maxima at 485nm and obeys Beer’s law in the concentration range of 2.5 – 20 g mL-1. The absorbance was found to be increasing linearly with increasing concentration of Ibutilide, which is corroborated by the calculated correlation coefficient value of 0.9995 (n=8). The molar absorptivity and Sandell’s sensitivity are 3.611x104 1 mole cm-1 and 0.024 g cm-2 respectively. The slope and intercept of equation of regression line are 0.0403 and 0.0144 respectively. The limit of detection was 0.561 g mL-1 The optimum experimental parameters for the reaction were studied and also validity of the described procedure was assessed. The statistical analysis of results revealed high accuracy and good precision. The proposed method was successfully applied to the determination of Ibutilide Active Pharmaceutical Ingredient (API) in bulk drug substance form. Copyright@ International Science Community Association