<p class=\"MsoNormal\" style=\"text-align: justify; tab-stops: 36.0pt;\"><em><span style=\"font-size: 10.0pt; line-height: 115%; mso-bidi-font-family: \'Times New Roman\';\">Glycorrhiza glabra is a widely used medicinal plant. Glycorrhiza glabra (liquorice) is a well known herb in traditional Indian and Chinese medicines. It is cultivated mainly in Jammu &amp; Kashmir, Punjab and sub Himalayan tracts in India to cure certain diseases like cough, Asthma, Bronchitis, Peptic ulcer, Arthritis, Allergic complaints and steroid therapy. In this investigation we have evaluated antibacterial activity of&nbsp; acetone extract of stem of Glycorrhiza&nbsp; glabra against ten clinically important bacterial strains such as Escherichia coli, Bacillus megaterium, Bacillus cereus, Bacillus subtilis, Bacillus amyloliquifacience, Salmonella typhi, Salmonella paratyphi A, Salmonella&nbsp; paratyphi B, Pseudomonas aeruginosa and Proteus vulgaris by disc diffusion method. Result showed maximum antibacterial activities against Bacillus megaterium with zone of inhibition of 23 mm and minimum against Salmonella typhi A with zone of inhibition of 11 mm. Our preliminary phytochemical and antibacterial analysis of acetone extract of Glycorrhiza glabra confirmed the presence of secondary metabolites such as tannin, flavonoids, terpenoids and steroids etc. This indicates that antimicrobial activities may be due to presence of secondary metabolites. Hence, this plant can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals antibacterial drug.</span></em></p>