<p class=\"MsoNormal\" style=\"margin-bottom: .0001pt; mso-layout-grid-align: none; text-autospace: none;\"><span style=\"font-family: \'Times New Roman\',\'serif\';\">&nbsp;Section name- Biological Sciences&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;Section No. 3</span></p>
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<p class=\"MsoNormal\" style=\"margin-bottom: .0001pt; text-align: center; mso-layout-grid-align: none; text-autospace: none;\" align=\"center\"><strong><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">&nbsp;</span></strong></p>
<p class=\"MsoNormal\" style=\"margin-bottom: .0001pt; text-align: center; mso-layout-grid-align: none; text-autospace: none;\" align=\"center\"><strong><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">Efficient Callus and Multiple shoot induction in <em>Brassica juncea</em> var; Pusa Jai Kisan</span></strong></p>
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<p class=\"MsoNormal\" style=\"text-align: center;\" align=\"center\"><span style=\"font-family: \'Times New Roman\',\'serif\';\">Neha Trivedi* and Ashutosh Dubey</span></p>
<p class=\"MsoNormal\" style=\"margin-bottom: 0.0001pt; text-align: center;\" align=\"center\"><span style=\"font-size: 12.0pt; font-family: \'Times New Roman\',\'serif\';\">Department of Biohemistry, CBSH &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></p>
<p class=\"MsoNormal\" style=\"margin-bottom: 0.0001pt; text-align: center;\" align=\"center\"><span style=\"font-size: 12.0pt; font-family: \'Times New Roman\',\'serif\';\">G.B. Pant University of Agriculture &amp; Technology, Pantnagar U.S. Nagar 263 145</span></p>
<p class=\"MsoNormal\" style=\"margin-bottom: 0.0001pt;\"><span style=\"font-size: 12.0pt; font-family: \'Times New Roman\',\'serif\';\">&nbsp;</span></p>
<p class=\"MsoNormal\" style=\"text-align: center;\" align=\"center\"><strong><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">Presenter E-mail:</span></strong><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\"> nehatrivedi36921@gmail.com</span></p>
<p class=\"MsoNormal\" style=\"margin-bottom: .0001pt; text-align: justify; mso-layout-grid-align: none; text-autospace: none;\"><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">Brassica oilseed species hold the third position among oilseed crops. The oilseed Brassica species cultivation has increased tremendously during the last decade and by now it is the second largest contributor to the world supply of vegetable oil. In most of the reports regarding tissue culture and genetic transformation of <em>Brassica </em>species, <em>B. juncea </em>has not yet been explored. In the present study an efficient regeneration protocol for <em>B.juncea </em>has been established for callus induction and multiple shoot induction which can be use to established for its use in transformation experiments. Different concentrations of hormones and their combination were applied to most commonly used explants viz; hypocotyls and cultured on MS medium. Two phytohormones, auxins (Naphthalene acetic acid and Indole acetic acid) and cytokinins (Benzylaminopurine and Kinetin) were used for callus induction and multiple shoot induction in <em>Brassica juncea</em> variety Pusa Jai Kisan. The frequency and amount of callus induction was higher when explants was treated with NAA (2.5ppm) + BAP (1.0ppm), at this concentration it showed maximum CIF (Callus Induction frequency) which was 91.66. The explants were treated with different concentrations of NAA and BAP for callus induction. At a particular combination of hormone there was shoot induction takes place in callus. Maximum Shoot Induction Frequency (SIF), was observed at concentration NAA (1.0ppm) + BAP (1.0). This protocol can be further explore for transformation of <em>Brassica juncea.</em></span></p>
 
<p class=\"MsoNormal\" style=\"margin-bottom: .0001pt; text-align: justify; mso-layout-grid-align: none; text-autospace: none;\"><strong><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">Keywords:</span></strong><em><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\"> Brassica juncea</span></em><span style=\"font-size: 12.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">, Callus induction, multiple shoot induction.</span></p>