<p class=\"Default\" style=\"text-align: justify; line-height: 115%;\"><em><span style=\"font-size: 10.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">Thuja occidentalis is a monoecious coniferous plant belongs to the family <strong>Cupressacae</strong>. It has high medicinal importance plant. </span></em><em><span style=\"font-size: 10.0pt; line-height: 115%;\">&nbsp;</span></em><em><span style=\"font-size: 10.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\">The plant is highly used by rural people in curing various disorders. Thuja occidentalis has an effective natural origin that has a tremendous future for research as the novelty and applicability of Thuja occidentalis are still hidden</span></em><em><span style=\"font-size: 10.0pt; line-height: 115%; font-family: \'Berlin Sans FB\',\'sans-serif\';\">.</span></em><em><span style=\"font-size: 10.0pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\';\"> In this study, the antibacterial activity of Thuja occidentalis leaves with&nbsp; methanol solvent were tested against different Gram positive and Gram negative bacterial strains such as Bacillus subtilis, Bacillus megaterium, Bacillus amyloliquifaciens, Proteus vulgaris and Salmonella typhi by Disk Diffusion method. All tested bacterial organisms were susceptible to methanol extracts showing zone of inhibition with different bacterial strains were in range of 10 to 27 mm with methanolic extract. The result shows that Thuja occidentalis leaves have potential of maximum antibacterial activity againstBacillus amyloliquifaciens with zone of inhibition of 27 mm and minimum antibacterial acitivity againstBacillus subtilis with zone of inhibition of 13mm. The extracts showed significant results against both gram positive and gram negative bacterial strains. This antibacterial properties of this used plant against various organism is depend upon various phytochemical constituents present in Thuja occidentalis leaves. There are no report found on antibacterial activity of Thuja accidentalis.</span></em></p>