 
<p class=\"Body\" style=\"margin: 12pt 0cm 0pt; tab-stops: 162.75pt;\"><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-GB; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-GB\">Fluorosis is a progressive degenerative disorder which is known to affect predominantly the skeletal systems, teeth and also the structure and function<span style=\"mso-spacerun: yes;\">&nbsp; </span>of various organs like skeletal muscle,brain &amp; spinal cord,ovaries and testies.</span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-US; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-US\">However, the effect of fluoride on </span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-GB; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-GB\">genes</span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-US; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-US\"> is far from clear.</span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-GB; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-GB\">The aim of present study is to examine the effects of fluoride on three genes viz.,Caspases which plays a central role in execution phase of cell apoptosis; P53( Tumor protein ) which is a transcription factor and is encoded in humans by TP53,which conserves stability by preventing mutation ;BCl2 ia a prototype for a family of mammalian genes and the proteins they produce.They govern mitochondrial outer membrane permeabilisation and can be either pro-apoptopic or anti apoptopic.Adult female albino mice of Swiss Strain<span style=\"mso-spacerun: yes;\">&nbsp; </span>weighing about 30 mgms were given a dose of<span style=\"mso-spacerun: yes;\">&nbsp; </span>aqueous Sodium Fluoride 20 mgms per kg Bwt per day<span style=\"mso-spacerun: yes;\">&nbsp; </span>and maintained for 14 days.After DNA extraction quality check<span style=\"mso-spacerun: yes;\">&nbsp; </span>and quantification was done</span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-US; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-US\">.The results obtained for the Caspase gene under study did not vary for the normal and test mice, we get the same size of amplified products for both the cases. The PCR products obtained were further sequenced and matched but there w</span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-GB; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-GB\">ere</span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-US; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-US\"> no differences in their sequences.In conclusion, our findings revealed that we need to elaborate our study with<span style=\"mso-spacerun: yes;\">&nbsp; </span>more genes thats can be influenced and damaged by different forms of fluorine compounds.<span style=\"mso-spacerun: yes;\">&nbsp; </span></span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-GB; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-GB\">We</span><span style=\"line-height: 115%; font-family: \'Times New Roman\',\'serif\'; font-size: 12pt; mso-ansi-language: EN-US; mso-bidi-font-family: Calibri; mso-hansi-font-family: Calibri;\" lang=\"EN-US\"> assume may be substantially more evident effect was caused by other fluoride compounds compared to simple fluoride ion released by sodium fluoride.</span></p>
 