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<p class=\"MsoBodyText2\" style=\"margin-bottom: 0.0001pt; text-align: justify; line-height: 150%;\"><strong><span style=\"color: green;\">ABSTRACT</span></strong><span style=\"color: black;\"><span>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></p>
 
<p class=\"MsoNormal\" style=\"margin-bottom: 0.0001pt; text-align: justify; line-height: 150%;\"><span style=\"font-size: 12pt; line-height: 150%; font-family: \'Times New Roman\',\'serif\'; color: black;\"><span>&nbsp;</span><span>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span>Among the plant growth promoting rhizobacteria (PGPR), great attention has been paid to the genus <em>Azotobacter,</em>and their role in increasing the growth and health of plants. In the present study,six samples of rhizospheric soil were taken to isolate <em>Azotobacter </em>from the<span>&nbsp; </span>ten different<span>&nbsp; </span>villages of<span>&nbsp; </span>Pravara area. Isolation of <em>Azotobacter</em> spp was carried out using Ashbys liquid and were purified<span>&nbsp; </span>further. The strains were identified through microscopical and biochemical tests and the results obtained classified the spp in, <em>A. vinelandii</em><span>&nbsp; </span>, <em>A. chroococcum</em> ,<em>A. beijerinckia,A.macrocytogenes,A.agilis.A.insignis.</em></span><span style=\"font-size: 12pt; line-height: 150%; font-family: \'Times New Roman\',\'serif\'; color: black;\"> Results strongly supports that the <em>Azotobacter </em>spp<span>&nbsp; </span>in combinations C+M+I and B+V+A are<span>&nbsp; </span>significantly effective<span>&nbsp; </span>to improve the crop yield . Their efficiency equal to the recommended dose of fertilizers. Highest gibberllic acid in production AzT<sub>1</sub>, kinetin in A<sub>Z</sub>T<sub>2</sub>, NAA in A<sub>Z</sub>T<sub>1 </sub>A<sub>Z</sub>M<sub>3 </sub>andIAA</span><span style=\"font-size: 12pt; line-height: 150%; font-family: \'Times New Roman\',\'serif\';\"> This supported that mutual association of <em>Azotobacter</em> spp. lead to synthesis of growth hormone, nitrogen, phosphate uptake and modification in rhizospheric interactions with respect to wheat varieties.<span style=\"color: black;\">. Results strongly supports that the <em>Azotobacter </em>spp combinations C+M+I and B+V+A are<span>&nbsp; </span>significantly effective<span>&nbsp; </span>to improve the crop yield<span>&nbsp; </span>Their efficiency equal to the recommended dose of fertilizers.<span>&nbsp; </span>The </span><em>Azotobacter</em><span style=\"color: black;\"> spp are capable of producing two phytohormones . Rhizosphere colonizing bacteria including <em>A. chroococcum</em> that posses the ability to enhance plant growth when applied to seeds, roots or tubers are called plant growth promoting rhizobacteria </span></span><span style=\"font-size: 12pt; line-height: 150%; font-family: Symbol; color: black;\"><span>(</span></span><span style=\"font-size: 12pt; line-height: 150%; font-family: \'Times New Roman\',\'serif\'; color: black;\">PGPR</span><span style=\"font-size: 12pt; line-height: 150%; font-family: Symbol; color: black;\"><span>)</span></span><span style=\"font-size: 12pt; line-height: 150%; font-family: \'Times New Roman\',\'serif\'; color: black;\">.<span>&nbsp; </span>They further observed that plant response to inoculation with PGPR enhance nitrogen and parameters such as plant dry weight, development, morphology of root system, grain yield, protein and mineral nutrient content. They further observed that plant response to inoculation with PGPR enhance nitrogen and parameters such as plant dry weight, development, morphology of root system, grain yield, protein and mineral nutrient content.</span></p>
<p><strong><span style=\"font-size: 12pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\'; color: black;\">Keywords: </span></strong><em><span style=\"font-size: 12pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\'; color: black;\">Azotobacter</span></em><span style=\"font-size: 12pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\'; color: black;\">spp</span><span style=\"font-size: 12pt; line-height: 115%; font-family: \'Times New Roman\',\'serif\'; color: black;\">,PGPR, Rhizospheric soil, HPLC, microbial population</span></p>