International Science Community Association Research Journal of Physical Sciences 14 2 2026 08 4 Evaluation of Methane Potential of Slaughterhouse Waste in the Urban Municipality of N'Zérékoré, Guinea Republic 1 8 EN Yombouno Faya Maurice 1 Département de Physique. Université de N’Zérékoré, BP 50, N’Zérékoré. Guinée Bayo Ibrahima 2 Centre de Recherche Marine et Côtière, Conakry, Guinée Summy PéLououéa 3 Département de Gestion des Ressources Naturelles. Université de N’Zérékoré. BP 50, N’Zérékoré, Guinée Keita Oumar 4 Département d’Hydrologie. Université de N’Zérékoré. BP 50, N’Zérékoré, Guinée Agbazo Noukpo 5 Département d’Hydrologie. Université de N’Zérékoré. BP 50, N’Zérékoré, Guinée > Research Paper 2025 11 24 2026 08 4 This study focuses on optimizing biogas production from biodegradable waste from the slaughterhouse in the urban commune of N'Zérékoré, Guinea. To achieve this objective, we rigorously identified and quantified three main types of waste: rumen contents, blood, and wastewater. Next, an in-depth physicochemical characterization of these substrates was carried out. The results reveal high moisture content (56 to 87%), a high carbon/nitrogen (C/N) ratio ranging from 24 to 32, and a high proportion of degradable organic matter. These results indicate conditions conducive to anaerobic digestion. In addition, an experiment was conducted over a period of 39 days in 4.5-liter laboratory digesters at a controlled mesophilic temperature (29 to 36°C) to evaluate the methanogenic potential of waste at different dilution ratios. The rumen content produced up to 2.493 ml of biogas per kilogram of dry matter with an optimal dilution ratio of 1/1.5, while blood reached a maximum volume of 3.415 ml/kg under similar conditions. Co-digestion with pig manure showed lower potential, suggesting the need for specific optimizations for this mixture. These findings demonstrate the energy value of local slaughterhouse waste for biogas production, offering a renewable and decentralized solution in rural areas. This recovery promotes sustainable management of organic residues while contributing to energy independence and reducing the environmental impacts of animal waste in Guinea. Copyright@ International Science Community Association