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A Prospective Study on Bacterial Isolates causing Neonatal Septicemia and their Sensitivity Pattern in a Tertiary level Hospital of Dhaka, Bangladesh

Author Affiliations

  • 1 East West University, Plot No- A/2, Main Road, Jahurul Islam City, Aftabnagar, Dhaka-1212, BANGLADESH
  • 2 Institute of Child Health and Shishu Sasthya Foundation Hospital (ICH and SSFH), 6/2 Barabagh, Mirpur-2, Dhaka, BANGLADESH

Int. Res. J. Medical Sci., Volume 3, Issue (2), Pages 16-21, February,28 (2015)


Now a day’s high bacterial resistance is creating an alarming situation in the developing and also in the developed countries. This prospective study has been done to isolate the bacterial pathogens of neonatal septicemia and their resistance pattern to establish the best antimicrobial regimens. Here 961 suspected cases of septicemia were included who admitted to the Special Care Neonatal Unit (SCANU) of the selected tertiary care hospital from June 1, 2012 to December 31, 2013 in Dhaka, Bangladesh. Demographic and clinical data were collected. Blood culture was done in all cases. Sensitivity was tested by Kirby Bauer Disc Diffusion techniques. Clinically early onset sepsis was diagnosed in 559 and late onset sepsis in 402 cases. However, culture positivity was found only in 125 cases, among them, early onset sepsis in 73.6% and late onset sepsis in 26.4% of cases. Culture positivity showed a highly significant relationship with the age of the neonates. Blood culture was positive in more cases (74/464) of hospital delivery compared to home delivery (51/497), which was statistically significant. Here Gram negative organisms (88.0%) were predominating. Most of the drugs (Ampicillin, Gentamicin, Ceftriaxone, Cefotaxime, Ceftazidime, Amikacin) were almost 100% resistant against Acinetobacter, Pseudomonas aruginosa, Streptococcus pneumonie, Gram negative bacilli, Enterobacter Cloacae, Klebsiella pneumoniae. Ampicillin was found to be 100% resistant to Gram negative cocci. Only Imipenem (84.9%), Meropenem (92%) and Ciprofloxacin (93.6%) were found to be sensitive against most of the bacterial isolates. To overcome this situation antibiotic selection by regular monitoring is essential.


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