International Science Community Association International Research Journal of Medical Sciences 14 2 2026 08 28 How can ancient Ayurvedic Principles and Formulations be Validated, Optimised, and Commercialised using modern Biotechnology tools for Global healthcare? 8 11 EN Singh Shivani 1 Department of Microbiology, KBS Commerce and Nataraj Professional Sciences College, Vapi, Gujarat, India Dubey Pinky 2 Department of Microbiology, KBS Commerce and Nataraj Professional Sciences College, Vapi, Gujarat, India Vakilwala Mita 3 Department of Microbiology, KBS Commerce and Nataraj Professional Sciences College, Vapi, Gujarat, India Mishra Alpana 4 Department of Microbiology, KBS Commerce and Nataraj Professional Sciences College, Vapi, Gujarat, India Makrani Mizba 5 Department of Microbiology, KBS Commerce and Nataraj Professional Sciences College, Vapi, Gujarat, India Pandalai Uma 1 Department of Biological Sciences, Oakland University, Rochester, MI, USA Tripathi Sunandini 1 Department of Biotechnology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, UP, India > Review Paper 2026 1 5 2026 08 28 Ayurveda, one of the world’s oldest holistic healthcare systems, offers a rich repository of therapeutic formulations grounded in natural products and systems-level understanding of human health. However, its global acceptance has long been limited by the lack of standardised scientific validation. This study explores how modern biotechnology can bridge that gap by providing rigorous tools to authenticate, optimise, and commercialise Ayurvedic formulations for contemporary healthcare needs. Using biotechnological approaches such as DNA barcoding, metabolomics, in-vitro bioassays, CRISPR-enabled plant improvement, and advanced fermentation technologies, Ayurvedic preparations can be made more consistent, potent, and globally market-ready. The methodology focuses on selecting classical formulations, validating their botanical identity, profiling their bioactive components, assessing pharmacological efficacy, improving extraction and production yields, and developing standardised, reproducible products. By merging traditional wisdom with cutting-edge biotech tools, this study aims to transform Ayurveda into evidence-backed, scalable therapeutics capable of contributing meaningfully to global health systems. Ultimately, the integration of these two knowledge systems not only elevates the scientific credibility of ancient remedies but also opens new pathways for sustainable innovation, equitable healthcare access, and the commercialisation of next-generation natural therapeutics. Copyright@ International Science Community Association