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Study of Some Bacterial Isolates Associated with Leukocytospermia in Asthenospermic Patients in Hilla City, Iraq

Author Affiliations

  • 1Medical Microbiol.-Bacteriology, Babylon University-College of Science, IRAQ
  • 2 Medical Microbiol.-Bacteriology, Babylon University-College of Medicine,IRAQ

Int. Res. J. Medical Sci., Volume 1, Issue (3), Pages 1-11, April,28 (2013)


One hundred asthenospermic seminal fluid specimens were collected from 100 infertile males who referred to Babylon maternity and children hospital-infertility center. It was found that 70 seminal fluid specimens had asthenospermia with leukocytospermia (First group). The rest 30 seminal fluid specimens had asthenospermia without leukocytospermia (Second group). Regarding semen cultures the results showed that 61(87.1%) of specimens of First group revealed positive bacterial culture, whereas 9(12.9%) specimens of First group showed no bacterial growth even after incubation of 48 hours. All semen specimens of Second group revealed negative bacterial culture. Gram positive bacteria constituted 44(62.9%) while gram negative bacteria constituted 26(37.1%) of isolates. Coagulase negative staphylococci (CoNS) represented by Staphylococcus epidermides and Staphylococcus saprophyticus) were the common type of bacterial isolates 25(35.7%) followed by Staphylococcus aureus 19(27.2%), Escherichia coli 12(17.1%), Enterobacter aerogenes 8(11.4%), Acinetobacter spp 4(5.7%) and Moraxella spp 2(2.9%). The virulence factors of bacterial isolates were investigated. The results showed that all S. aureus isolates, 18(72%) isolates of CoNS and 5(41.7%) of E. coli isolates and 4(50%) of E. aerogenes isolates produce hemolysin. Colonization factor antigens (CFA/) were detected in all isolates of S. aureus, CoNS, E. coli, E. aerogenes, Acinetobacter spp. and Moraxella spp. (CFA/) were expressed in 10(52.6%) isolates of S. aureus, 8(32%) isolates of CoNS, 8(66.7%) isolates of E. coli, 6(75%) isolates of E. aerogenes, 2(50%) isolates of Acinetobacter spp. and 1(50%) isolate of Moraxella spp. Lipase produced by 15(78.9%) and 7(28%) isolates of S. aureus and CoNS isolates respectively, while 9(75%) isolates of E. coli , 7(87.5%) isolates of E. aerogenes and 1(50%) isolate of Moraxella spp. produce lipase. Only 7(36.8%) isolates of S. aureus and 5(41.7%) isolates of E. coli were found to be protease producers. The effects of some antibiotics on bacterial isolates were investigated. The results showed that, the bacterial isolates were highly susceptible to imipenem, meropenem and ciprofloxacin whereas exhibited moderate resistance to amikacin, gentamycin and norfloxacin. On the other hand bacterial isolates revealed high rate of resistance to amoxicillin, ceftizoxime, ceftazidime, cefamandole, cefepime, amoxicillin–clavulanic acid and tobramycin.


  1. Elbhar A., Male genital tract infection: the point of view of the bacteriologist, Gynecol. Obstet. Fertil.,33(9), 691-697 (2005)
  2. Onemu S.O. and Ibeh I.N., Studies on the significance of positive bacterial semen cultures in male fertility in Nigeria, Int. J. Fertil. Women Med.,46(4), 210-214 (2001)
  3. Li H.Y. and Liu J.H., Influence of male genital bacterial infection on sperm function, Zhonghoa. Nan. Ke. Xue., 8(6), 442-444 (2005)
  4. Bukharin O.V., Kuzimin M.D. and Ivanov I.B., The role of the microbial factor in the pathogenesis of male in fertility, Zh. Microbial. Epidemiol. Immunobiol.,2, 106-110 (2003)
  5. Diemer T., Ludwig M., Huwe P., Haler D.B. and Weidner W., Influence of genital urogenital infection on sperm function, Curr. Opin. Urol.,1(1), 39-44 (2000)
  6. Orji I., Ezeifeka G., Amadi E.S. and Okafor F., Role of enriched media in bacterial isolation from semen and effect of microbial infection on semen quality: A study on 100 infertile men, Pak. J. Med. Sci.,23(6), 885-888 (2007)
  7. Gdoura R., Kchaou W., Znazen A., Chakroun N., Fourati M., Ammar-Keskes L. and Hammami A., Screening for bacterial pathogens in semen samples from infertile men with and without leukocytospermia., 40(4), 209-218 (2008)
  8. Palayekar V.V., Joshi J.V., Hazari K.T., Shah R.S. and Chitlange S. M., Comparison of four nonculture diagnostic tests for Chlamydia trachomatis infection, J. Assoc. Physicians, India, 48, 481-483 (2000)
  9. Behre H.M., Yeung C.H. and Nieschlag E., Diagnosis of male infertility and hypogonadism. In: Andrology: male reproductive health and dysfunction (Nieschlag E., Behre H. M., eds.), Berlin: Springer-Verlag, 87, 110 (1997)
  10. Trum J.W., Mol B.W.J., Pannekoek Y., Spanjaard L., Wertheim P., Bleker O.P. and Veen van der F., Value of detecting leukocytospermia in the diagnosis of genital tract infection in subfertile men, Fertil. Steril.,70, 315-319 (1998)
  11. World Health Organization (WHO), WHO laboratory manual for the examination of human semen and sperm- cervical mucus interaction, 4th edition; Cambridge,Cambridge University Press. UK (1999)
  12. Collee J.G., Fraser A.G., Marmino B.P. and Simons A., Mackin and McCartney Practical Medical Microbiology, 14th ed., The Churchill Livingstone, Inc. USA (1996)
  13. Mac Faddin J.F., Biochemical tests for the identification of medical bacteria. 3rd ed., The Williams and WilkinsBaltimor, USA (2000)
  14. Forbes B.A., Daniel F.S. and Alice S.W, Bailey and Scott's Diagnostic microbiology, 12th ed., Mosby Elsevier company, USA (2007)
  15. Clinical and Laboratory Standards Institute (CLSI),Performance Standards for Antimicrobial Susceptibility Testing; Twentieth Informational Supplement M100-S20; 30(1), Replaces M100-S19, 29(3), 1-157 (2010)
  16. Shefi S. and Turek P.J., Definition and current evaluation of subfertile men, Int. Braz. J. Urol. Rio de Janeiro,23(4),212-222 (2006)
  17. Jiao Y., Long S.D. and Grange W.X., The observation of chronic prostatitis using national institutes of health, J.Hosp. Chin. Acad. Trad. Med.,8(2),127-129 (2002)
  18. Golshani M., Taheri S., Eslami G., Suleimani A.A., Fallah F. and Goudarzi H., Genital tract infection in asymptomatic infertile men and its effect on semen quality, Iranian J. Publ. Health,35(3), 81-84 (2006)
  19. Philip A.S. and Folstad I., Do bacterial infections cause reduced ejaculate quality? A meta-analysis of antibiotic treatment of male infertility, Behav. Ecolo.,14(1), 40-47 (2003)
  20. Chimura T. and Saito H., A clinical study of asymptomatic bacteriospermia, Jpn. J. Antibiot.,43(1), 139-146 (1990)
  21. Riegel P., Ruimy R., Briel D., Prevost G., Jehl F., Bimet F., Christen R. and Monteil H., Corynebacterium seminali sp. Nov., new species associated with genital infection in male patients, J. Clinic. Microbiol.,33(9), 2244-2249 (1995)
  22. Virecoulon F., Wallet F., Fruchart A.F., Rigot J.M., Peers M.C., Mitchell V. and Courcol R.J., Bacterial flora of the low male genital tract in patients consulting for infertility, Andrologia, 37(5), 160-165 (2005)
  23. El-Shamy H.A., Bacteriology of chronic secretory otitis media in children. J. Egypt public health association,Lxv III, 495-505 (1993)
  24. Rodin D.M., Larone D. and Goldstein M., Relationship between semen culture, leukospermia and semen analysis in men undergoing fertility evaluation, Fertil. Steril.,79(3), 1555-1558 (2003)
  25. Ikechukwu O., George E., Sabinus A.E. and Florence O., Role of enriched media in bacterial isolation from semen and effect of microbial infection on semen quality,Pak. J. Medic. Sci.,23(6), 885-888 (2007)
  26. Fraczek M., Szumala A.K., Jedrzejczak P., Kamieniczna M. and Kurpisz M., Bacteria trigger oxygen radical release and sperm lipid peroxidation in vitro model of semen inflammation, Fertil. Steril.,88(40), 1076-1085 (2007)
  27. Eiichi A., Monden K., Mitsuhata R., Kariyama R. and Kumon H., Biofilm formation among methicillin resistant staphylococcus aureus isolates from patients with urinary tract infection, Acta. Medica. Okayama.,58(4), 207-214 (2004)
  28. Yassin H., Chronic otitis media microbiological and epidemiological study, Msc. thesis. College of medicine, University of Basrah , Iraq (1990)
  29. Stimac G.D., Agreb A.N. and Gorica M.A., New prospect for chronic prostatitis, J. Acta. Clin. Croat. Urol.,40(2), 109-116 (2001)
  30. Lee J.C., Microbiology of prostate, J. Clin. Dis.,1, 159-163 (2000)
  31. Merino G., Carranza L.S., Murrieta S., Rodriguez L., Cnevas E. and Moran C., Bacterial infection and semen characteristics in infertile men, Arch. Androl.,35(1), 43-47 (1995)
  32. Alwash B.H., A bacteriological and clinical study of patients with benign prostatic hyperplasia and or chronic prostatitis, Msc. thesis, College of medicine, Babylon university Iraq., (2006)
  33. Neilands J.B., Siderophores: Structure and function of microbial Iron transport compounds, J. Biolog. Chem.,270(45), 26723-26726 (1995)
  34. Dinges M.M., Orwin P.M. and Schlievert P.M., Exotoxins of Staphylococcus aureus,J. Clin. Microbiol,13(1), 16-34 (2000)
  35. Vergis E.N., Shankar N., Chow J.W. and Hayden M.K.,Association between the presence of enterococcal virulence factors gelatinase, hemolysin and enterococcal surface protein and mortality among patients with bacteremia due to Enterococcus faecalis,J. Clin. Infect. Dis.,35(5), 570-575 (2002)
  36. Bartels E.D., Nielsen J.E., Lindgaard M.L. and Hulten L.M., Endothelial lipase is highly expressed in macrophages in advanced human atherosclerotic lesions Atherosclerosis, J. Appl. Microbiol., 12, 65-73 (2007)
  37. Ofek I., Hasty D.L. and Doly R.J., Bacterial Adhesion. Am. Soc. Microbiol. Washington, DC, 63-69 (2002)
  38. Maria A.R., Zeus S., Aysen L.E. and Valerio M.N., Commensal and pathogenic Escherichia coli use a common pilus adherence factor for epithelial cell colonization. Proc. National Acad. Sci. USA (2007)
  39. Dan P.N., Treatment of acute and chronic bacterial prostatitis caused by Staphylococcus epidermidis. J. Clin. Infect. Dis.,43, 147 (2005)
  40. Aggarwal A., Kanna S. and Arova U., Characterization biotyping antibiogram and klebocin typing of Klebsiella, Indian J. Med.,57(2), 68-70 (2003)
  41. Romolo G., Facep L., Warcester M.A. and Gideon B., Complicated urinary tract infection: Risk stratification, clinical evaluation and evidence-based antibiotic therapy for out management. Update. J. American Health Consultants,68(30), 230-274 (2004)
  42. Ang J.Y., Ezike E. and Asmar B., Antibacterial resistant, Indian, J. pediatrics,71(3), 229-239 (2004)
  43. Dulawa J., Poland K. and Katowice J., Urinary tract infection and metabolic diseases, Ann. Academia Medicae Bialotocensis.,10(3), 45-50 (2003)
  44. Al-Shukri M.S., A study of some bacteriological and genital aspects of Acinetobacter isolated from patients in Hilla-city. Msc. thesis. College of Science, Babylon University, Iraq (2003)
  45. Tzelepi E., Giakkoupi P., Ofianou D., Loukova S.V., kemeroglou A. and Tsakris A., Detection of extendedspectrum -lactamases in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes,J. clin. Micorbiol., 38(3), 542-546 (2000)
  46. Dumarche P., Dechamps C., Sirot D., Chanal C., Bonnet R. and Sirot J., TEM derivative–producing Enterobacter aerogenes Strains: dissemination of a prevalent clone, Antimicrobiol. Agent Chemother,46(4), 1128-1131(2000)
  47. Varon E., Levy C., Dela R. and Boucherat M., Impact of antimicrobial therapy on nasopharyngeal carriage of Streptococcus pneumoniae, Haemophilus influenzae andBranhamella catarrhalis in children with respiratory tract infections, J. Clin. Infect. Dis.,31, 477-481 (2000)
  48. Brooks G.F., Butel J.S. and Morse S.A., Medical microbiology. 23rd ed. Lange Medical books, McGraw-Hill Companies.USA (2004)
  49. Bisiklis A., Tsiakiri E. and Alexiou-Danieel S., Phenotypic detection of Pseudomonas aeruginosa producing metallo-- lactamases, European Society of clinical microbiology and infectious disease, 15th European congress of clinical microbiology and infectious disease, 1134, 1-11 (2005)
  50. Watanabe A., Takahashi H., Kikuchi T., Kobayashi T., Gomi K., Fujimura S., Tokue Y. and Nukiwa T., Comparative in vitro activity of S-4661, a new parenteral carbapenems and other antimicrobial agents against respiratory pathogens, J. Antimicrob. Agents Chemother,46, 184-187 (2000)
  51. Nomura S. and Nagayama A., In vitro antibacterial activity of S-4661, a new parenteral carbapenems, against urological pathogens isolated from patients with complicated urinary tract infections, J. Chemother,14,155-160 (2002)
  52. Khorshed P.A., Bacteriological study of some pathogens causing urinary tract infection in pateints attending Azadi general hospital in Kirkuk city.Msc thesis, College of Education – Tikret Univercity.Iraq (2005)
  53. Al-Muhanna A.S., Identification of bacteria causing pneumonia in three medical centers in Najaf. M.Sc. thesis. Kufa University, Iraq (2001)
  54. Al-Nuaimi E.M., Urinary tract infection in pregnant women .M.Sc. thesis. College of science. Al-Mustansiriya university, Iraq (2002)
  55. Al-Hamawandi J.A., Bacteriological and immunological study on infants pneumonia at Babylon governorate, M.Sc. thesis. College of Science, Al-Mustansiriya University, Iraq (2005)
  56. Galimand M., Lambert T. and Courvalin P., Emergence and dissemination of a new mechanism of resistance to aminoglycosides in gram- negative bacteria: 16s rRNA methylation, J. Biol. Chem.,10(1), 243-331(2005)
  57. Rachid S.A., Witte V.M. and Hacker J.Z., Effect of subinhbitory antibiotic concentration on Intracellular adhesion expression in biofilm-forming Staphylococcus epidermidis,J. Antimicrob. Agents Chemother, 32, 755-760 (2000)
  58. Kurt G. and Naber I., Which fluoroquinolones are suitable for the treatment of urinary tract infection, Intern, J. Antimicrob. Agent.,17, 331-341 (2001)
  59. Donnell J.A. and Gelone S.P., Antimicrobial therapy: fluoroquinolones, J. Clin. Infect. Dis.,4, 488-513 (2000)
  60. Klligore D., March K.L. and Blance B.J., Risk factors for community acquired ciprofloxacin resistant Escherichia coli urinary tract infection, J. Ann. Pharmacol.,38(7),1148-1152 (2004)
  61. Jacoby G.A., Walsh K.E., Mills D.M., Walker J., Oh H., Robicsek A. and Hooper D.C., A qnrB, another plasmid mediated gene for quinolone resistance, Antimicrob. Agents and Chemother, 50(4), 1178-1182 (2006)