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Isolation and Studying the Effect of Protein Fractions of White Cabbage (Brassica oleracea var. capitata) on Some Biochemical Parameters in Experimental Diabetic Rats

Author Affiliations

  • 1 Department of Chemistry, College of Science, Mosul University, Mosul, IRAQ

Int. Res. J. Medical Sci., Volume 1, Issue (11), Pages 17-22, December,28 (2013)


The research was an attempt to isolate and study the active protein compounds from the aqueous extract of white cabbage(Brassica oleracea var. capitata) from local market in Iraq, using different biochemical techniques. Two compounds (A and B) were isolated using gel filtration chromatography for the precipitate produced by ammonium sulphate precipitation. The results predicted that, glucose level was lowered by intraperitoneally administration of the concentrated aqueous extract and the protein compound (B) compared with oral administration. The comparative molecular weight of the isolated protein active compound (peak B), was found to be 4797.34 Dalton. The study was also included the effect of the aqueous extract and the protein compound (B) in four doses (50, 75, 100 and 125 mg/kg body weight) on certain blood constituents in normal and alloxan induced diabetic rats. In normal rats, the blood glucose level was significantly lowered by crude aqueous extract and the protein compound in a dose (75 mg/kg body weight) compared with control group. In addition, the protein compound in a same dose showed a significant decrease in serum cholesterol and total lipids level. In diabetic rats, the protein compound in a dose of (75mg/kg body weight) showed a significant decrease in serum glucose, cholesterol and total lipids levels.


  1. Kurilich A.C., Jeffery E.H., Juvik J.A., Wallig M.A. and Klein P.L., Antioxidant capacity of different broccoli (Brassica oleracea) genotypes using the oxygen radical absorbance capacity (ORAC) assay, Journal of Agriculture and Food Chemistry, 50(18), 5053-5057 (2002)
  2. Jagdish S.AK, Upadhyay A.B.B, Singh B, Singh K.P and Mathura R. AK., Antioxidant phytochemicals in cabbageBrassica oleracea L. var. capitata), Scientia Horticulturae, 108, 233–237 (2006)
  3. Kusznierewicza B., Bartoszekb A., Wolskaa L. and Drzewieckic J., Gorinsteind Sh; Namiesnika J., Partial characterization of white cabbages (Brassica oleraceavar. capitata. alba) from different regions by glucosinolates, bioactive compounds, total antioxidant activities and proteins, LWT, 41, 1–9 (2008)
  4. Garg M.C. and Bansal D.D., Protective antioxidant effect of vitamin C and E in streptozotocin induced diabetic rats, Indian Journal of Experimental Biology, 38,101-104 (2000)
  5. Faltusová Z., Kuera L. and Ovesná J., Genetic diversity of Brassica oleracea var. capitata gene bank accessions assessed by AFLP, Electronic Journal of Biotechnology, 14(3)(2011)
  6. Rasal V.P., Shetty B.P., Sinnathambi A. and Yeshmaina S., Antihyperglycaemic And Antioxidant Activity Of Brassica Oleracea In Streptozotocin Diabetic Rats, The Internet Journal of Pharmacology, 4(2), (2006)
  7. Passing H. and Bablok W., A new biochemical procedure for testing the equality of measurements from two different analytical methods, J. Clin. Chem. Clin. Biochem., 24, 863-869 (1983)
  8. Tietz N.W., Text book of clinical chemistry,3rd ed .C.A. Burtis, E.R. Ashwood, W.B. Saunders, 809-856 (1999)
  9. Chabral and Chardonnet, Cited by Geloson Torol, Philip G. Ackermann, Drac. Clin. Chem. Little, Brown and Company, Boston (1975)
  10. Steel R.G. and Torrie J.H., Principles and Procedures of Statistics a Biometrical Approach, 2nd ed., McGraw-Hill, Inc., Singapore (1980)
  11. Robyt J.F. and White B.J., Biochemical Techniques, Theory and Practice. Books/Cole Publishing Company California, USA (1987)
  12. Srinivas P. and Patil PA., Hypoglycemic activity of Brassica oleracea var. gongylodes in normal and diabetic rats, Fitoterapia, 5(4), 301-303 (1993)
  13. Komatsu W, Miura Y. and Yagasaki K., Suppressionof hypercholesterolemia in hepatoma-bearing rats by cabbage extract and its component, S-methyl-L-cysteine sulfoxide, Lipids, 33, 499–503 (1998)
  14. Murray K., Granner D.K., Mayes P.A. and Rodwell V.W., "Harper‘s Biochemistry", 24th ed., 581-599 (1996)
  15. Heo H.J. and Lee C.Y., Phenolic phytochemicals in cabbage inhibit amyloid. Protein-induced neurotoxicity, LWT - Food Science and Technology, 39, 331–337 (2006)
  16. Khan A., Safdar M. and Khan M.M.A., Effect of various doses of cinnamon on lipid profile indiabetic individuals, Pakistan J. Nutr., 2(5), 312-319 (2003)
  17. Nammi S., Boni M.K., Lodagala S.D. and Behara R.B.S., The juice of fresh leaves of Catharanthus voseus linn reduces blood glucose in normal and alloxan diabetic rabbits, BMC Complementary and Alternative Medicine, 3(4),1-4 (2003)
  18. Ahmad, T.Y.; Al-Jawadi, Z.A.M.; Bedewe, L.F., Isolation and studying the effect of proteinous fractions of Unripe banana fruit (Musa paradisiaca L.) on some biochemical parameters in experimental diabetic rats, Raf. Jour. Sci., 19(1), 1-8 (2008)
  19. Grover, J.k.; Yadav, S.P.; Vats, V., J., Effect of feeding Murraya koeingii and Brassica juncea diet on [correction] kidney functions and glucose levels in streptozotocin diabetic mice, Ethnopharmacol, 85, 1–5 (2003)
  20. Yokozawa T., Kim H.Y., Cho E.J., Yamabe N. and Choi J.S., Protective effects of mustard leaf (Brassica juncea) against diabetic oxidative stress, J. Nutr. Sci. Vitaminol, 49(2), 87–93 (2003)