International Science Community Association Research Journal of Pharmacutical Sciences 15 1 2026 06 30 Colorimetric phytochemical analysis of Tinospora Cordifolia and FTIR study of its Medicinal sample Giloy Ghanvati 1 12 EN Gupta Swaroopa Rani N. 1 Department of Chemistry, Brijlal Biyani Science College, Amravati, Maharashtra, India > Research Paper 2026 9 11 2026 06 30 Tinospora cordifolia (giloy) is a herbaceous vine of the family Menispermaceae indigenous to tropical regions of the Indian subcontinent. It has been used in Ayurveda to treat various disorders. Tinospora cordifolia contains diverse phytochemicals, including alkaloids, phytosterols, glycosides, tinosporide, and other mixed chemical compounds. During the 2020-22 COVID-19 outbreak in India, the Ministry of AYUSH recommended use of Giloy as a home remedy for immune support. Giloy extract was assessed for its phytochemical and antibacterial properties, to find out the bioactive components responsible for such activity. In Ayurveda, it is said to be the best rejuvenating herb. Acute toxicity studies of aqueous extract of Guduchi reports that it does not produce any toxic effect. The medicinal herb has to be used in an appropriate dose as prescribed by a qualified physician to get medicinal effects. With the wide range of actions and abundant components, Guduchi is a real treasure among herbal drug source. Medicinal applications of Guduchi in countering various disorders and it's use as anti-oxidant, anti-hyperglycemic, anti-hyperlipidemic, hepatoprotective, cardiovascular protective, neuroprotective, osteoprotective, radioprotective, anti-anxiety, adaptogenic, analgesic, anti-inflammatory, anti-pyretic, anti-diarrheal, anti-ulcer, anti-microbial, and anti-cancer have been well established. Present study deals with Colorimetric phytochemical analysis of Tinospora Cordifolia (Giloy leaf extract) and FTIR study of its medicinal sample Giloy Ghanvati. Colorimetric phytochemical analysis of Tinospora Cordifolia (Giloy leaf extract) involves preparation of standard GiloyGhanvati solution, Ferric Chloride solution and Giloy leaf Solution. Different systems were prepared. Absorbance of standard Giloy solution was taken at different wavelength. And λmax was calculated. Which was 420 nm. Absorbance of standard Giloy solution of different concentrations were taken at 420 nm and Calibration plot was prepared. Then absorbance of Giloy leaf solutions were taken at 420 nm and concentration of Giloy in Giloy leaf was calculated from calibration plot. Tinospora cordifolia which contains diverse phytochemicals, including alkaloids, phytosterols, glycosides, tinosporide, and other mixed chemical compounds reduces ferric ions and production of violet coloration by addition of ferric chloride to dilute solution of Giloy takes place and the concentration of Giloy was investigated colorimetrically. The method is simple, rapid and precise. Interpretation of FTIR Spectra of GiloyGhanvati medicinal sample shows presence of various functional groups such as O-H stretching - Alcohol, Carboxylic acid, O-H bending - Alcohol, N-H stretching - Amine salt, C=C stretching - α, β- unsaturated ketone and C=O stretching - Conjugated acid. Copyright@ International Science Community Association