International Science Community Association Research Journal of Pharmacutical Sciences 14 2 2025 12 30 Therapeutic Potential of Ebselen-Driven Wound Repair in Mercury-Induced Cell Injury in NRK52E Renal Epithelial Cells 38 51 EN Zaware Sandeep 1 Bioanalytical Research Department, Synergen Bio pvt. Ltd. Pune, Maharashtra, India Bhoite Sangram 2 Bioanalytical Research Department, Synergen Bio pvt. Ltd. Pune, Maharashtra, India Kadam Akshay 3 Bioanalytical Research Department, Synergen Bio pvt. Ltd. Pune, Maharashtra, India Lahoti Neel 4 Bioanalytical Research Department, Synergen Bio pvt. Ltd. Pune, Maharashtra, India Anjana V.S. 1 Department of Pharmaceutical Chemistry, Mar Dioscorus College of Pharmacy, Alathara, Thiruvananthapuram, India Sreeja S. 2 Department of Pharmaceutical Chemistry, Mar Dioscorus College of Pharmacy, Alathara, Thiruvananthapuram, India Sreekumar Anagha 3 Department of Pharmaceutical Chemistry, Mar Dioscorus College of Pharmacy, Alathara, Thiruvananthapuram, India Basheer Bismi S. 4 Department of Pharmaceutical Chemistry, Mar Dioscorus College of Pharmacy, Alathara, Thiruvananthapuram, India Gupta Swaroopa Rani N. 1 Department of Chemistry, Brijlal Biyani Science College, Amravati, Maharashtra, India Gupta Pragati Kumari 1 Department of Zoology, Maharani Janki Kunwar College, Bettiah, West Champaran, Bihar-845438, India . Hafizurrahman 2 Department of Zoology, Maharani Janki Kunwar College, Bettiah, West Champaran, Bihar-845438, India > Research Paper 2025 11 4 2025 12 30 Mercury, a toxic environmental pollutant, is readily available in the biogeochemical cycle and poses serious health risks even at trace levels due to its tendency to bioaccumulate. Among its forms, methylmercury (MeHg) is the most toxic, capable of crossing both the placental barrier and blood-brain barrier. This study investigates the cellular toxicity of MeHg on normal rat kidney epithelial cells (NRK52E) and evaluates the protective potential of Ebselen, an organic selenium compound with antioxidant and metal-chelating properties. NRK52E cells were exposed to 0.1 µM and 1 µM concentrations of MeHg, both alone and in combination with 10 µM Ebselen. Multiple assays including wound healing for cell migration, DCFDA for reactive oxygen species (ROS) generation, Luminex multiplex assay for key kidney toxicity markers, invasion assay and cell cycle analysis were performed to assess the extent of toxicity and protection. MeHg exposure led to a significant increase in ROS levels, accompanied by dysregulation of cell adherence and wound healing. These molecular changes were associated with disrupted cell behaviour, impaired wound healing, and altered cell cycle progression, all indicating cellular toxicity. However, co-treatment with Ebselen significantly reversed these effects. ROS levels were reduced and cell cycle distribution improved. Ebselen’s ability to restore cellular homeostasis demonstrates its strong protective effect against MeHg-induced nephrotoxicity. This study highlights the potential of Ebselen as a therapeutic agent for mitigating mercury-induced kidney cell damage. It emphasizes the importance of early detection of nephrotoxic effects and timely antioxidant intervention to prevent long-term renal impairment caused by environmental toxins like MeHg. Copyright@ International Science Community Association