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Toxicity of quaternary mixtures of metals to aquatic microbial community

Author Affiliations

  • 1Department of Microbiology, Federal University of Technology Owerri, P.M.B.1526, Owerri, Imo State, Nigeria
  • 2Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli Campus, P.M.B. 02 Ihiala, Anambra State, Nigeria
  • 3Department of Microbiology, Federal University of Technology Owerri, P.M.B.1526, Owerri, Imo State, Nigeria
  • 4Department of Microbiology, Federal University of Technology Owerri, P.M.B.1526, Owerri, Imo State, Nigeria

Int. Res. J. Environment Sci., Volume 6, Issue (3), Pages 30-37, March,22 (2017)


The toxicities of quaternary mixtures of metal ions [Cd(II), Co(II), Zn(II) and Ni(II)] against microbial community of river water were assessed using inhibition of INT-dehydrogenase activity as endpoint and uniform design concentration ratios. The effective concentrations (EC50) were estimated using logistic concentration-response model. The toxicity of the individual metal ion was ranked as Cd(II) > Co(II) > Zn(II) > Ni(II). In comparison to observed toxicities, the concentration addition (CA) and independent action (IA) models predicted the combined toxicities of the mixtures with varying accuracy. The deviations from accurate prediction of the mixture toxicities indicate possible synergistic and antagonistic effects of the mixtures. However, the model deviation ratios (MDR) based on 50% effective concentrations (EC50s) for most mixtures lie between 0.5 and 2.0. Thus, the combined action of the mixtures were considered to be additive.


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