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Decolorisation of Reactive Voilet-1 by Novel Isolate Bacillus Cereus Strain CMGS-4

Author Affiliations

  • 1Dept of Microbiology, Gulbarga University, Kalaburagi, Karnataka, India
  • 2Dept of Microbiology, Gulbarga University, Kalaburagi, Karnataka, India
  • 3Dept of Microbiology, Gulbarga University, Kalaburagi, Karnataka, India

Int. Res. J. Environment Sci., Volume 5, Issue (9), Pages 45-54, September,22 (2016)


Microorganisms play an important role in biodegradation of pollutants including recalcitrant azo dyes. Out of 20 bacterial isolates, bacillus cereus strains- CMGS-4 isolated from textile mill effluent, Sholapur, Maharashtra gave excellent result, in the utilizing reactive violet-1 as a sole source of carbon. The strain identified as Bacillus cereus sps (Gene bank accession num-633716), by conventional and 16S rRNA sequencing methods. Optimized the biotic and abiotic parameters for the maximum decolorization of RV-1 by bacterial isolate. Organism decolorized initially added RV-1 up to 200mg/L within 12 hours and could decolorized more than 70% at 800mg/L and 60% when dye concentration increased to 1000mg/L within 24 hours. The isolate had a capacity to decolorize more than 80% in a wide range of pH 7 to10 and temperature (25 to 45o C) however it decolorizes RV-1 better only in 1% salt concentration and also shown decolorization of five structurally different azo reactive dyes in mixed (in equal quantity) within 24 hrs. So it could be a better candidate for the decolorisation of textile effluents containing reactive azo dyes.


  1. Correia V.M., Stephenson T. and Judd S.J. (1994), Characterization of textile wastewater- a review., Environmental Technology, 15, 917-929
  2. Couto S.R. (2009)., Dye removal by immobilized fungi., Biotech Adv., 27, 227-235.
  3. Hong Y. and Guo J. et. al. (2007)., Reduction and Partial Degradation Mechanisms Of Naphthylaminesufonic Azo Dye Amaranth By Shewanella Decolorizations S12., Applied Microbial Biotechnol, 75, 647-654.
  4. Saraswathy K. and Balakumar S. (2009)., Biodecolorization of Azo Dye (Pigmented red 208) Using Bacillus firmus and Bacillus laterosporus., J. Biosciences Technol., 1, 1-7.
  5. Harshad Lade, Avinash Kadam, Diby Paul and Sanjay Govindwar (2015)., Biodegradation And Detoxification f Textile Azo Dyes By Bacterial Consortium Under Sequential Microaerophilic/Aerobic Processes., EXCLI Journal, 14, 158-174
  6. Jayarajan M., Arunachalam R. and Annadurai G. (2011)., Agricultural wastes of jackfruit peel nanoporous adsorbent for removal of rhodamine dye., Asian J. Applied Sci., 4, 263270.
  7. Vigneswaram M., Govindarajan B., Shanmugaraja K. and Prabakaran V. (2010)., Biotreatment for Effective Degradation and Decolorization of Textile Effluent Using novel spore forming Bacillus Sp., J. Ecobiotechnol, 2(11), 1-5.
  8. Thakur J.K., Paul S., Dureja P., Annapurna K., Padaria J.C., and Gopal M. (2014)., Degradation of Sulphonated Azo Dye by Red HE7B by Bacillus Sp. and Elucidation of Degradation Pathway., Curr Microbial, 69, 183-191.
  9. Saitou and Nei M. (1987)., The neighbor-joining method: a new method for reconstructing Phylogenetic trees., Mole. Biol. Evolution, 4(4), 406-425.
  10. Takezaki N., Rzhetsky A. and Nei M. (1995)., Phylogenetic test of the molecular clock and linearized trees., Mole. Biol. Evolution, 12(5), 823-833.
  11. Tamura K., Nei M. and Kumar S. (2004)., Prospects for inferring very large phylogenies by using the neighbor-joining method., Proceedings of the National Academy of Sciences, 101(30), 11030-11035.
  12. Koichiro Tamura, Glen Stecher, Daniel Peterson, Alan Filipski and Sudhir Kumar (2013)., MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0., Mol Biol Evol., 30(12), 2725-2729.
  13. Oturkar C.C., Nemade H.N., Mulik P.M., Patole M.S. and Hawaldar R.R. (2011)., Mechanistic investigation of decolorization and degradation of Reactive Red 120 by 494 Bacillus lentus BI377., Bioresour. Technol., 102, 758-764.
  14. Bheemaraddi M.C., Patil S., Shivannavar CT. and Gaddad S.M. (2014)., Isolation and characterization of Paracoccus sp. GSM2 capable of degrading textile azo dye Reactive Violet 5., The Scientific World J., 410704, doi:10.1155/2014/410704.
  15. Joe M.H., Lim S.Y., Kim D.H. and Lee I.S. (2008)., Decolorization of reactive dyes by Clostridium bifermentans SL186 isolated from contaminated soil., World J Microbiol Biotechnol., 24, 2221-2226, 2- Asad.
  16. Moosvi S., Kher X. and Madamwar D. (2007)., Isolation, characterization and decolorization of textile dyes by a mixed bacterial consortium JW-2., Dyes and Pigments, 74(3), 723-729.
  17. Asad S., Amoozegar M.A., Pourbabaee A.A., Sarbolouki M.N. and Dastgheib S.M.M. (2007)., Decolorization of textile azo dyes by newly isolated Halophilic and halotolerant bacteria., Bioresource Technol, 98, 2082-2088.
  18. Ali H. (2010)., Biodegradation of Synthetic Dyes - A Review., Water Air Soil Pollut, 213, 251-273.
  19. Jain K., Shah V., Chapla D. and Madamwar D. (2012)., Decolorization and degradation of azo dye--Reactive Violet 5R by an acclimatized indigenous bacterial mixed culturesSB4 isolated from anthropogenic dye contaminated soil., J Hazard Mater, 213-214, 378-386.
  20. Rajee O. and Patterson J. (2011)., Decolorization of azo dye (Orange MR) by and autochthonous bacterium, Micrococcus sp. DBS 2., Indian J Microbiol., 51, 159-163.
  21. Kadpan I.K., Kargi F., McMullan G. and Marchant R. (2000)., Effect of environmental conditions on biological decolorization of textile dye stuff by C. Versicolor., Enzyme. Microb. Technol., 26, 381-387.