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Studies on the Antioxidative stress responses of fungicides carbendazim and mancozeb in seedlings of brassica (Brassica compestrisL.)

Author Affiliations

  • 1Post-Graduate Department of Biotechnology, Lyallpur Khalsa College, GT Road, Jalandhar-144001 Punjab, India
  • 2Post-Graduate Department of Biotechnology, Lyallpur Khalsa College, GT Road, Jalandhar-144001 Punjab, India

Int. Res. J. Environment Sci., Volume 5, Issue (2), Pages 57-62, February,22 (2016)


The present study is an attempt to understand the effect of different concentrations of fungicides-carbendazim and mancozeb on the various antioxidative enzymes in developing seedling of brassica. Seeds were treated with different conc. (0.01-0.05%) of fungicides for 3 hours and then allowed to grow for 10 days in an incubated chamber. Treatment of fungicides triggered a defence machanism of various antioxidative enzymes viz. ascorbate perosidase, guaiacol peroxidase, catalase and superoxide dismutase and it was observed that the level of catalase and peroxidase decreased at higher conc of fungicides as compared to control samples. Mancozeb treatment, however showed an increase in the conc of catalase activity. An overall increase in the activity of superoxide dismutase was observed in samples treated with carbendazim, mancozeb individually as well as in combination thus suggesting that plant induces various antioxidative enzymes as protective defence mechanism against fungicide stress. These results indicate that the application of fungicide singaly or in combination in brassica seedlingsis deleterious at higher dosages and should be used judiciously in current agriculture.


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