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Biofixation potential of Carbon dioxide by Fresh water species of Chlorella and Closteriopsis

Author Affiliations

  • 1Division of Environmental Science, Department of Botany, University of Calicut, Malappuram, Kerala-673635, India
  • 2Division of Environmental Science, Department of Botany, University of Calicut, Malappuram, Kerala-673635, India

Int. Res. J. Environment Sci., Volume 5, Issue (2), Pages 51-56, February,22 (2016)


Microalgae based seizure of CO2 can be an effective means of carbon sequestration, owing to their higher photosynthetic efficiency, faster growth rate and higher biomass production compared to plants. Several members including Chlorella have been brought in this direction and the present study is an attempt to introduce new algal members in carbon assimilation. Pure cultures of Chlorella and Closteriopsis were maintained in Bolds Basal medium. For experimentation, 1 litre each of the respective algal cultures was added to 4 litres each of the culture medium kept in 3 glass tanks of size 18x18x24 cm. First tank was maintained as such and treated as control. The second and third tanks were supplied with air and CO2 gas respectively at constant flow rate. pH, Dissolved Oxygen and free carbon dioxide content of all the treatment sets were monitored at an interval of 3 hours and cell count, cell size and biomass were worked out at an interval of 6 hours. All the 3 sets were kept under observation for a period of 48 hours and the results are reported. The results of the present study showed that in spite of slightly acidic pH and higher free CO2 content, the Chlorella sp. exhibited higher rate of DO production, cell count, cell size and biomass content in treatment sets containing CO2 supply than Closteriopsis sp. The bending of the tips of the cells of Closteriopsis sp. in CO2 treated set is indicative of the stressful condition developed within the system.


  1. Akinola O.O. (2014)., Review of the Role of Plant inCarbondioxide Sequestration Globally using ChlorophyllII or Leaf Index, J Environ Earth Sci ., 4, 22-30.
  2. Wang B., Li Y.Q., Wu N. and Lan C.Q. (2008)., CO2 biomitigationusing microalgae, ApplMicrobiol Biotechnol.,79(5), 707718.
  3. Sahoo D., Elangbam G. And Devi S.S. (2012)., Usingalgae for carbon dioxide capture and bio-fuel productionto combat climate change., Phykos., 42(1), 3238.
  4. Kativu E. (2011)., Carbon Dioxide Absorption UsingFresh Water Algae and Identifying Potential Uses ofAlgal biomass, Dissertation For Masters of Science inEngineering, University of the Witwatersrand,Johannesburg 1-133.
  5. Chen C.Y., Yeh K.L., Aisyah R., Lee D.J. and Chang J.S.(2011)., Cultivation, photobioreactor design andharvesting of microalgae for biodiesel production: acritical review, Bioresour. Technol., 102(1), 7181.
  6. keffer J.E. and Kleinheinz G.T. (2002)., Use of Chlorellavulgaris for CO2 mitigation in a photobioreactor, J IndMicrobiol Biotechnol., 29(5), 275-80.
  7. Nichols H.W. and Bold H.C. (1965)., Trichosacinapolymorpha Gen. et sp. NOV, Journal of Phycology. 1,34-38.
  8. Yue L. and Chen W. (2005)., Isolation and determinationof cultural characteristics of a new highly CO2 tolerantfreshwater microalgae, Energ. Convers. Manage., 46(11),18681876.
  9. Ponnuswamy I., Madhavan S., Shabudeen S. and ShobaU.S. (2014)., Resolution of Lipid Content from AlgalGrowth in Carbon Sequestration Studies, Int. j. Sci. adv.technol., 67, 23-32.
  10. Harun I., Yahya L., Chik M.N., Kadir N.N.A. and PangM.A.M.A. (2014). Effects of Natural Light Dilution onMicroalgae Growth, Int. J. Chem. Eng. Appl., 5(2), 112-116., undefined
  11. Karampudi S. and Chowdhury K. (2011)., Effect ofMedia on Algae Growth for Bio-Fuel Production, Not SciBiol, 3(3), 33-41.
  12. Ooka H., Ishii T., Hashimoto K. and Nakamura R.(2014)., Light-Induced Cell Aggregation of Euglenagracilis Towards Economically Feasible BiofuelProduction, RSC Adv., 4, 20693-20698.
  13. Pisal D.S. and Lele S.S. (2005)., Carotenoid productionfrom microalga, Dunaliella salina, Indian J. Biotechnol.,4, 476-483.
  14. Avron M. and Ben-Amotz A. (1992)., Dunaliella:Physiology, Biochemistry and Biotechnology, CRCPress, Boca Raton.