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Ascertaining the genetic basis of variants in Justicia adhatoda L. collected from Tamil Nadu using ISSR- PCR Markers

Author Affiliations

  • 1St. Xavier′s College (Autonomous), Palayamkottai, Tamil Nadu, India
  • 2Plant Molecular Biology Research Unit, Department of Botany, St. Xavier′s College (Autonomous), Palayamkottai, Tamil Nadu, India

Int. Res. J. Biological Sci., Volume 9, Issue (1), Pages 17-20, February,10 (2020)


In the present investigation genetic variability of Justicia adhatoda L. belonging to the family Acanthaceae was carried out. The healthy fresh young leaves were collected from four different regions of Tamil Nadu. The DNA was isolated with standard protocol and the ISSR analysis was studied with five primers. The primers produced distinct bands in each population. The polymorphic loci number was 12 and percentage was 36 respectively. In between the population the genetic distance ranged from 0.3610 to 0.0625 and 0.9394 to 0.6970 respectively. 1.3636 was the mean value of overall observed number of alleles and 1.2788 was the effective number of alleles. The overall value for the genetic diversity was 0.1553 and value for Shannon information index was 0.2243. The UPGMA dendrogram represents the phylogenetic relationship between different accessions of Justicia adhatoda. The present study helps to find out the accession which has more genetic variability for the conservation in near future.


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