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MicroRNAs Specific Primer Design using miRNA Design Tool

Author Affiliations

  • 1Veer Narmad South Gujarat University, Surat, Gujarat, India
  • 2Veer Narmad South Gujarat University, Surat, Gujarat, India

Int. Res. J. Biological Sci., Volume 6, Issue (8), Pages 44-46, August,10 (2017)


MicroRNAs (miRNAs) are tiny (only 18-24 nucleotide long) non-coding RNAs which involved in post-transcriptional regulation of gene expression in multi-cellular organisms by affecting both translation of mRNAs and the stability. There are several different methods used for quantification of miRNAs like Northern Blotting, quantitative RT-PCR, Microarray etc. but, quantitative RT-PCR is used as the standard that is used to validate & confirmation of the results of various methods. The design of primers for miRNA qRT PCR is very much difficult because of short length of miRNA, which size is the very much near to the length of normal PCR primers. The miRNA Design Tool is based on the Universal Probe Library (ULP) probes to design primer(s) for miRNA detection. The tool is a software based and easy method for design of working primers for target specific miRNA for qRT-PCR. The application is available as online service by AstridBio.


  1. E.G. (2015)., Entrez Gene: MicroRNA 145., Retrieved 2015-01-26.
  2. Götte M., Mohr C., Koo C.Y., Stock C., Vaske A.K., Viola M., Ibrahim S.A., Peddibhotla S., Teng Y.H., Low J.Y., Ebnet K., Kiesel L. and Yip G.W. (2010)., miR-145-dependent targeting of junctional adhesion molecule A and modulation of fascin expression are associated with reduced breast cancer cell motility and invasiveness., Oncogene, 29(50),6569-6580.doi:10.1038/onc.2010.386.PMID 20818426.
  3. UPL (2017)., Universal Probe Library., https://lifescience. 05/06/2017
  4. Larsson E., Fredlund Fuchs P., Heldin J., Barkefors I., Bondjers C., Genové G., Arrondel C., Gerwins P., Kurschat C., Schermer B., Benzing T., Harvey S.J., Kreuger J. and Lindahl P. (2009)., Discovery of microvascular miRNAs using public gene expression data: miR-145 is expressed in pericytes and is a regulator of Fli1., Genome Medicine, 1(11), 108. doi:10.1186/gm108.PMC 2808743. PMID19917099.
  5. Zhang J., Guo H., Zhang H., Wang H., Qian G., Fan X., Hoffman A.R., Hu J.F. and Ge S. (2011)., Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene., Cancer, 117(1),86-95. doi:10.1002/cncr.25522.PMC 2995010.PMID 20737575.
  6. Sachdeva M., Zhu S., Wu F., Wu H., Walia V., Kumar S., Elble R., Watabe K. and Mo Y.Y. (2009)., p53 represses c-Myc through induction of the tumor suppressor miR-145., Proceedings of the National Academy of Sciences of the United States of America, 106(9), 3207-12. Doi:10.1073/pnas.0808042106. PMC 2651330. PMID 19202062.
  7. Slaby O., Svoboda M., Fabian P., Smerdova T., Knoflickova D., Bednarikova M., Nenutil R. and Vyzula R. (2007)., Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer., Oncology, 72(5-6), 397-402. doi:10.1159/000113489.PMID 18196926.
  8. Starczynowski D.T., Morin R., McPherson A., Lam J., Chari R., Wegrzyn J., Kuchenbauer F., Hirst M., Tohyama K., Humphries R.K., Lam W.L., Marra M. and Karsan A. (2011)., Genome-wide identification of human microRNAs located in leukemia-associated genomic alterations., Blood, 117(2),595-607. doi:10.1182/blood-2010-03-277012.PMID 20962326.
  9. Zsolt C., Julianna H., Zoltan S., Eva V., Zoltan H., Erzsebet S., Attila V., Balazs D., Maria B., Attila H., Balint D., Zsolt T., Laszlo N. and Balint A. (2013)., A Versatile Method to Design Stem-Loop Primer-Based Quantitative PCR Assays for Detecting Small Regulatory RNA Molecules., PLoS One, 8(1), e55168. doi: 10.1371/journal.pone.0055168. PMCID: PMC3561390
  10. Busk P.K. (2014)., A tool for design of primers for microRNA-specific quantitative RT-qPCR., BMC Bioinformatics, 15, 29. doi: 10.1186/1471-2105-15-29.