International Science Community Association International Research Journal of Biological Sciences 2278-3202 6 12 2017 12 10 Gene transfer in eukaryotic cells: current applications and implications 40 44 EN Gaur Jyoti 1 Department of Science, Aishwarya College of Education, Jodhpur, Rajasthan, India Naruka Kavita 2 Department of Zoology, J.N.V. University, Jodhpur, Rajasthan, India Ghadigaonkar Pritesh 1 Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India Pandirkar Akshay 2 Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India Varkey Johnson 3 VIVA College of Arts, Commerce and Science, Viva College Road, Virar West, Vasai – 401303, MS, India Jangam Prajal 4 Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India Bandekar Pradnya 5 Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India Khedekar Prakash 6 Ecocare Ecology Consultant, 10 Hedvekar wadi no. 2, D.S. Babrekar Marg, Gokhale Road, Dadar west Mumbai 400028, MS, India Patil Omkar 7 VIVA College of Arts, Commerce and Science, Viva College Road, Virar West, Vasai – 401303, MS, India Chikkappaiah Lava 1 Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India Nayaka M.A. Harish 2 Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India . Mahadevaiah 3 Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India K.S. Venkatesh 4 Dept. of Studies in Sugar Technology, Sir M. Visvesvaraya Post Graduate Centre, University of Mysore, Tubinakere, Mandya-571402, India Kumar Dinesh 5 Department of Chemistry, Bipin Bihari Science PG College, Jhansi, UP, India Ganguly Arindam 1 Department of Microbiology, Vidyasagar University, Midnapore- 721102, West Bengal, India Mandal Asish 2 Department of Botany, Ramananda College, Bishnupur, Bankura-722122, West Bengal, India Khan Mohammad Ali 3 Department of Mathematics, Ramananda College, Bishnupur, Bankura-722122, West Bengal, India Dutta Tapas Kumar 4 Department of Zoology, Bankura Sammilani College, Bankura- 722102, West Bengal, India Raha Subrata 5 Department of Botany, Sidho-Kanho-Birsha University, Purulia-723104, West Bengal, India Mohapatra Pradeep Kumar Das 6 Department of Microbiology, Vidyasagar University, Midnapore- 721102, West Bengal, India Calderón Alejandro Alonso-Calderón 1 Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico Juárez Juana DeisySantamaría- 2 Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico Vargas Jorge R. Omaire-Vargas 3 Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico Robles Esmeralda Vidal-Robles 4 Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico Caselis Jenaro LeocadioVarela-Caselis 5 Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico Cruz D. 6 Facultad de Ingeniería Química de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla, Mexico Bravo Edith Chávez- 7 CICM del Instituto de Ciencias de la Benemérita Universidad Autónoma de Puebla, 72000, Puebla Asmelash Bruh 1 College of Agri. and Environmental Sciences, School of Animal and Range Sciences, Post Box: 138, Haramaya University, Dire Dawa, Ethiopia Brhane Haftom 2 College of Natural and Computational Science; Department of Biology, Haramaya University, Ethiopia > Review Paper 2017 6 16 2017 12 10 Gene transfer is indispensable to modify the genome of organisms and enhance their overall performance as compared to the average performance of their ancestors. Gene transfer in eukaryotic cells gives way to decipher the genotypes responsible for different traits, agricultural enhancement and therapeutic strategy. The three commonly used gene transfer methods are physical, chemical and biological based ones. It is reported that the application of transgenenesis in livestock agriculture comprises of increment of growth rate, milk production and composition, feed usage and carcass composition, disease resistance, enhanced reproductive and proli& Copyright@ International Science Community Association 64257;cacy enhancement. Now days liposome mediated gene transfer is believed to be the most promising widely used gene transfer method in eukaryotic cells. In principle the DNA fragments encapsulated with liposomes adheres to the cell membranes which in turn will fuse with them to transfer genes or DNA fragments of interest. Thus the DNA fragment of interest enters into the nucleus of a cell that will later undergo reproduction. In order to achieve maximum transfection efficiency in eukaryotic cells it is essential to optimize several parameters including lipid to DNA charge ratio and DNA amount concentration. Liposome mediated gene transfer is relatively easy direct inexpensive reproducible and efficient.