International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 5 2 2016 February 10 Study of Swarming Motility and pilB Gene among some Clinical isolates of Pseudomonas aeruginosa infections 23 29 EN Awal Md. Shihabul 1 Department of Food Science and Nutrition, Hajee Mohammad Danesh Science , and Technology University, Dinajpur,Bangladesh Amin Md. Ruhul 2 Department of Agricultural and Industrial Engineering,Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh Haque Md. Raihanul 3 Department of Food Engineering, and Technology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh Hasan S.M. Kamrul 4 Department of Food Processing and Preservation Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh Pritesh Ghadigaonkar 1 Department of Zooology, Viva CollegeVirar (W), 401303, MH, Indi Akshay Pandirkar 2 Department of Zoology Kirti M. Doongursee College,Dadar (W), Mumbai, 400028, MH, India Aakash Kuwar 3 Department of Zoology, Institute of Science (IOS), Ma’am CamaRaod (Fort), Mumbai 400032, MH, India Prajal Jangam 4 Department of Zoology, M.D. College of Arts, Science and Commerce, Dr. S.S. Row Road Parel (E), Mumbai 400012, MH, India Tyas Utami 1 Department of Food and Agricultural Product Technology, Universitas Gadjah Mada, Jl. Flora No 1, Bulaksumur Yogyakarta, 55288, Indonesia Kasmiati 2 Faculty of Marine and Fischery Science, Hassaduddin University, Makassar, Indonesia Eni Harmayani 3 Department of Food and Agricultural Product Technology, Universitas Gadjah Mada, Jl. Flora No 1, Bulaksumur Yogyakarta, 55288, Indonesia Endang S Rahayu 4 Department of Food and Agricultural Product Technology, Universitas Gadjah Mada, Jl. Flora No 1, Bulaksumur Yogyakarta, 55288, Indonesia Shameran M. Tawfiq 1 Department of Soil and Water, College of agriculture University of Sallahaddin Erbil,Kurdistan,Iran > Research Paper 2015 12 28 2016 February 10 This study was conducted to investigate the relationship between the swarming motility and pilB gene, twenty seven isolates of Pseudomonas aeruginosa were identified according to morphologicl, cultural and biochemical characteristics ,the isolates were from blood, Urine and Burn (nine isolates form each infection source). The sensitivity test of these isolates to the antibiotics was showed that all isolates were resistant of the used antibiotics except Amikacin, Tobramicin and Flucloxacillin. The ability of these isolates for swarming were tested the result showed that all isolates were able to swarming (dendritic swarming pattern). There was differences in swarming shapes among same infection and different source of infections on the semisolid surface (0.5-1)% agar plates, then these isolates were tested to evaluated the present pilB gene (type IV fimbrial biogensis protein) by PCR technique using specific primers, the result of PCR showed that this gene was moderately distribution (51.8)% in all studied isolates, since the result showed that this gene was widely disseminated in urine isolates 8/9(89)% while was 3/9(33.3)% in each of blood and burns isolates. These results clearly favored conclusion that differences swarming shapes in Pseudomonas aeruginos a from isolate to another may be the isolates have difference virolence factors for infection and it is important in all types of infections while prevalence of pilB gene perhaps related to infection site, there are no relationship between pilB gene presence and swarming motility. Copyright@ International Science Community Association