International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 4 9 2015 September 10 Selected Philippine Herbal plant extracts as Angiogenesis inhibitors using Chick Chorioallantoic Membrane (CAM) assay 28 32 EN Ahirwar Ramesh 1 School of Studies in Botany, Vikram University Ujjain, M.P., 456010, INDIA Coronado F.F. 1 Environment and Biotechnology Division, Industrial Technology Development Institute, Department of Science and Technology, Esguerra R.L. 2 General Santos Avenue, Bicutan Taguig City 1631 Metro Manila, PHILIPPINES Sikat G.M. 3 Unciano N.M. 4 Peren T.M. 5 Beraye J.E. 6 Baruah C. 1 Department of Zoology, M.C. College, Barpeta, 781301, Assam, INDIA Gauhati University, Saikia P.K 2 Guwahati, Assam 781014, INDIA Salas G.M. 1 Department of Sciences, College of Arts and Sciences, Pampanga State Agricultural University, Magalang, Pampanga, PHILIPPINES Totaan E.V. 2 > Research Paper 2015 7 8 2015 September 10 The study was conducted to assess the phytochemical components and properties of herbal plant extracts, such as (a) sabungai (Gynura nepalensis DC), (b) pandan (Pandanus odoratissimus L.) and (c) tsaang-gubat (Carmona retusa (Vahl.) Masam.), as potential angiogenesis inhibitors using the Chick Chorioallantoic Membrane (CAM) assay. Phytochemical analysis showed that the three extracts were found to have alkaloids, tannins and flavonoids. Only pandan extract was assessed to contain anthraquinones and steroids. The treatments were individually compared to the positive control and negative control of the study. Statistical analysis showed significant difference on the mean number of branching points of eggs treated sabungai extract, pandan extract and tsaang-gubat as compared to negative control of the study. Sabungai extract was found significantly different to the positive control. On the other hand, no significant difference is seen in the mean number of the branching points of the eggs treated with pandan and tsaang-gubat extracts when individually compared to the positive control. Using the formula for CAM Vascularity that determines the inhibition properties of the extracts, it was found out that tsaang-gubat gained the highest inhibition of vascularity followed by pandan, then sabungai. Copyright@ International Science Community Association