International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Selected Philippine Herbal plant extracts as Angiogenesis inhibitors using Chick Chorioallantoic Membrane (CAM) assay

Author Affiliations

  • 1Department of Sciences, College of Arts and Sciences, Pampanga State Agricultural University, Magalang, Pampanga, PHILIPPINES

Int. Res. J. Biological Sci., Volume 4, Issue (9), Pages 28-32, September,10 (2015)


The study was conducted to assess the phytochemical components and properties of herbal plant extracts, such as (a) sabungai (Gynura nepalensis DC), (b) pandan (Pandanus odoratissimus L.) and (c) tsaang-gubat (Carmona retusa (Vahl.) Masam.), as potential angiogenesis inhibitors using the Chick Chorioallantoic Membrane (CAM) assay. Phytochemical analysis showed that the three extracts were found to have alkaloids, tannins and flavonoids. Only pandan extract was assessed to contain anthraquinones and steroids. The treatments were individually compared to the positive control and negative control of the study. Statistical analysis showed significant difference on the mean number of branching points of eggs treated sabungai extract, pandan extract and tsaang-gubat as compared to negative control of the study. Sabungai extract was found significantly different to the positive control. On the other hand, no significant difference is seen in the mean number of the branching points of the eggs treated with pandan and tsaang-gubat extracts when individually compared to the positive control. Using the formula for CAM Vascularity that determines the inhibition properties of the extracts, it was found out that tsaang-gubat gained the highest inhibition of vascularity followed by pandan, then sabungai.


  1. Roomi M.W., Ivanov V., Niedzwiecki A. and Rath M., Anti-Angiogenic and Antimetastatic Activities of a Novel Nutrient Formulation of Lysine, Proline, Ascorbic Acid, Proceedings of the 96th Annual Meeting of the AACR. (2005)
  2. Zetter B., Angiogenesis and Tumor Metastasis, Annual Review of Medicine,49, 407-24 (1998)
  3. Ji B., Chow W., Hsing A., Mclaughlin J.K, Dai Q., Gao Y., Blot W. and Fraumenijr J.F., Green tea consumption and the risk of pancreatic and colorectal cancers, International Journal of Cancer, 255-258.(1997)
  4. Mandl M., Kapeller B., Lieber R. and Macfelda K., The hen's egg test - chorio-allantoic-membrane (het-cam) assay as an alternative for animal experiments, (2010)
  5. Allera J.P., Potential Angiogenic and Anti-angiogenic of Alagaw(Prema odorata), Banaba (Lagerstomia speciosa) and Yerba Buena (Mentha cordifolia) using Chick Chorioallantoic Membrane (CAM) assay. Undergraduate Thesis. Pampanga Agricultural College, Magalang, Pampanga, (2007)
  6. Guevarra B.Q., A Guidebook to Plant Screening: Phytochemical and Biological Revised Edition (2005)
  7. Philippine Medicinal Plants, Sabungai, Retrieved October 11, 2013., (2013)
  8. Sasikumar J.M., Jinu U. and Shamna R., Antioxidant Activity and HPTLC Analysis of Pandanus odoratissimus L. Root. European J. of Biol. Sci., 1(2), 17-22 (2009)
  9. Penecilla G.L. and Magno C.P., Antibacterial activity of extracts of twelve common medicinal plants from the Philippines, J. Med. Plant. Res. 5(16), 3975-3981 (2012)
  10. Starr F., Starr K. and Loope L., Carmona retusa, Retrieved October 11, 2013, hiplants/reports/pdf/carmona_retusa.pdf, (2003)
  11. Kadioglu, O., Seo, E.J. and Efferth T. Targeting Angiogenesis By Phytochemicals, Medicinal and Aromatic Plants, 2(5), (2013)
  12. Kim MH, Flavonoids inhibit VEGF/bFGF-induced angiogenesis in vitro by inhibiting the matrix-degrading proteases, J Cell Biochem, 89(3), 529-38 (2003)
  13. Marienfeld C., Yoko Y. and Tushar P., Tannic acid inhibits angiogenesis induced by human cholangiocarcinoma cells, Gastroenterology 124(4), (2003)
  14. Xu H., Lui W., Chu C., Ng P., Wang C. and Rogers M., Anti-angiogenic effects of green tea catechin on an experimental endometriosis mouse, (2008)
  15. Mukhtar H. and Ahmad N., Tea polyphenols: prevention of cancer and optimizing health, The American Journal of Clinical Nutrition, 1698-1702 (2000)
  16. Foster, S. Green Tea (Camellia sinensis). Thorne Research, Inc., 200, (2007)
  17. Frei B. and Higdon J.V., Antioxidant Activity of Tea Polyphenols In Vivo: Evidence from Animal Studies. Proceedings of the Third International Scientific Symposium on Tea and Human Health: Role of Flavonoids in the Diet, The Journal of Nutrition, 3275-3284 (2003)
  18. Katiyar, S., Elmets, C., and Katiyar SK. Green tea and skin cancer: photoimmunology, Angiogenesis and DNA repair, (2007)
  19. Hoeben A, Landuyt B, Highley M, Wildiers H, Van Oosterom A, and De Bruijn E. Vascular Endothelial Growth Factor and Angiogenesis, Pharmacological Reviews, (2004)