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Isolation of Beneficial Microbes for the Biodegradation of Animal and Plant Oil in Fast Foods Wastewater Effluents

Author Affiliations

  • 1Environment and Biotechnology Division, Industrial Technology Development Institute, Department of Science and Technology,
  • 2General Santos Avenue, Bicutan Taguig City 1631 Metro Manila, PHILIPPINES

Int. Res. J. Biological Sci., Volume 4, Issue (9), Pages 10-16, September,10 (2015)


This study on the isolation and screening of aerobic microbes was developed for the treatment of fast foods wastewater effluents to reduce the oil and grease processed under aerobic conditions. The microorganisms were isolated from different fast foods wastewater effluents having both high oil and grease content, a big problem in the environment. From an initial 130 selected isolates, 54 were screened, then 28; thereafter eleven (11) isolates were found the most promising. The isolated microbes then identified as bacteria were screened and applied to the effluent and simulated wastewater for bioprocess treatment. These microbes were inoculated in nutrient broth for 24 hours at room temperature in an environmental shaker. These cultures serve as inoculum for treating contaminated fast foods wastewater in the laboratory for twenty four (24) hours. Analytical results showed that the highest reduction of oil and grease had 93.75% for the isolate JB8/8 (uncultured Basidiomycota) in wastewater effluents and isolate JB4/6 (Bacillus cereus) was 98.25 % in simulated wastewater.


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