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Natural Mycorrhizal Colonization of Plant Species growing in a Limestone Mine Spoil: Case Study from ACC, Coimbatore, India

Author Affiliations

  • 1Department of Botany, PSGR Krishnammal College for Women, Peelamedu, Coimbatore, Tamil Nadu 641004, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (7), Pages 73-78, July,10 (2015)


In the present study we examined the Arbuscular mycorrhizal association in forty seven angiospermic plants of the limestone mine spoils of Coimbatore, India. Forty one plants were colonized by AM fungi while Achyranthes aspera, Aerva lanata, Amaranthus viridis, Alternanthera pungens, (Amarantaceae), Carex speciosa (Cyperaceae)and Argemone mexicana (Papavaraceae) did not possess mycorrhizal association. Some of the common non-mycorrhizal plants, viz., Boerhaavia diffusa, Commelina bhengalensis and Trianthema portulacastrum were found to be associated with AM fungi. The soils of the study area were calcareous, sandy loam with a slight alkaline pH and low in available nutrients. A total of ten AMF spores were identified, which included the genus Acaulospora, Gigaspora and Glomus. Glomus was the most frequent genus observed in the study area.


  1. Singh A.N., Raghubanshi A.S. and Singh J.S., Plantations as a tool for mine spoil restoration, Curr. Sci., 82(12), 1436-1441 (2002)
  2. Lone M.I., He Z.L., Stoffella P.J. and Yang X., Phytoremediation of heavy metal polluted soils and water: Progress and perspectives, J. Zhejiang. Univ. Sci. B.,9(3), 210-220 (2008)
  3. Kavamura V.N. and Esposito E., Biotechnological strategies applied to the decontamination of soil polluted with heavy metals, Biotechnology Advances., 28, 61-69 (2010)
  4. Quoreshi A.M., The use of mycorrhizal biotechnology in restoration of disturbed ecosystem, In: Mycorrhizae: Sustainable agriculture and forestry, Springer, Oxford, (2008)
  5. Mukhopadhyay S. and Maiti S.K., Biofertiliser: VAM fungi-Future prospect for biological reclamation of mine degraded lands, Ind. J. Environ. Prot., 29(9), 801-808 (2009)
  6. Singh A.K., Jamaluddin, Status and diversity of arbuscular mycorrhizal fungi and its role in natural regeneration on limestone mined spoils, Biodiversitas., 12(2), 107-111 (2011)
  7. Chen B., Roos P., Zhu Y. and Jakobsen I., Arbuscular mycorrhizas contribute to phytostabilization of uranium in uranium mining tailings, J. Environ. Radioactiv., 99, 801-810 (2008)
  8. Rillig M.C. and Steinberg P.D., Glomalin production by an arbuscular mycorrhizal fungus a mechanism of habitat modification, Soil. Biol. Biochem., 34, 1371-1374 (2002)
  9. Koide R.T. and Dickie I.A., Effects of mycorrhizal fungi on plant populations, Plant Soil., 244, 307-317 (2002)
  10. Jackson M.L., Soil chemical analysis, Prentice Hall, New Delhi, 498 (1973)
  11. Sankaram A., A laboratory manual for agricultural chemistry, Asia Publishing House, 340 (1966)
  12. Phillips J.M. and Hayman D.S., Improved procedure for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungus for rapid assessment of infection, Trans. Br. Mycol. Soc., 55, 158-161 (1970)
  13. Koske R.E. and Gemma J.N., A modified procedure for staining roots to detect VA mycorrhizas, Mycol. Res., 92, 486-488 (1989)
  14. Gerdemann J.W. and Nicolson T.H., Spores of mycorrhizal Endogone species extracted from soil by wet seiving and decanting, Trans. Br. Mycol. Soc., 46, 235-244 (1963)
  15. Schenck N.C. and Perez Y., Manual for identification of VA mycorrhizal fungi. Synergistic Publications, Gainesville, Florida, USA., 1-286 (1987)
  16. Trappe J.M., Synoptic keys to the genera and species of zygomyceteous (vesicular-arbuscular) mycorrhizal fungi, Phytopathol., 72, 1102-1108 (1982)
  17. Schenck N.C. and Perez Y., Manual for the Identification of VA mycorrhizal fungi, Synergistic publications, Gainesville, Florida, 286 (1990)
  18. Jain R.K., Hasan N., Singh R.K. and Pandey P.N., Vesicular arbuscular mycorrhizal (VAM) associations in some weeds of forage legumes, Mycorrhiza News., 9-11 (1997)
  19. Muthukumar T. and Udaiyan K., Arbuscular mycorrhizas of plants growing in the Western Ghat region, Southern India, Mycorrhiza.,, 297-313 (2000b)
  20. Muthukumar T. and Udaiyan K., Influence of organic manures of arbuscular mycorrhizal fungi associated with Vigna unguiculata (L.) Walp in relation to tissue nutrients and soluble carbohydrate in roots under field condition, Biol. Fertil. Soils., 31, 114-120 (2000a)
  21. Klironomos J.N., Hart M.M., Gurney J.E. and Moutoglis P., Interspecific differences in the tolerance of arbuscular mycorrhizal fungi to freezing and drying, Can. J. Bot.,79, 1161-1166 (2001)
  22. Robinson-Boyer L., Grzyb I. and Jeferies P., Shifting the balance from qualitative to quantitative analysis of arbuscular mycorrhizal communities in field soils, Fungal Ecology, 1-9 (2009)
  23. Dhar P.P. and Mridha M.A.U., Arbuscular mycorrhizal associations in different forest tree species of Hazarikhil forest of Chittagong, Bangladesh, J. For. Res., 23(1), 115-122 (2012)
  24. Dhar P.P. and Mridha M.A.U., Biodiversity of arbuscular mycorrhizal fungi in different trees of Madhupur forest, Bangladesh, J. For. Res.,17(3), 201-205 (2006)
  25., (2015)