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Regeneration of Shoot from Nodal explants of Cucumis sativus considering different Hormonal concentration

Author Affiliations

  • 1Department of Biotechnology and Genetic Engineering, Islamic university, Kushtia, BANGLADESH

Int. Res. J. Biological Sci., Volume 4, Issue (7), Pages 48-52, July,10 (2015)


Cucumber (Cucumis sativus), one of the most economically important vegetable (cucurbit) crops, is eaten raw or cooked and is popularly used as salads in south Asia. Considering the nutrition value of Cucumis sativus, the present investigation was undertaken to develop a rapid and efficient in vitro multiplication and regeneration system of this species using in vitro nodal explants. Addition of cytokinin is essential to induce shoot formation from the explants. Of the two cytokinin tested, BAP was more effective than kinetin at concentration of 1.5mg/l yielded best response (87%) on shoot formation. Kinetin at 1.5mg/l showed the highest shoot regeneration frequency (53%). When combination of auxin with IAA (0.5 mg/l) + BAP (1.0 -5.0 mg/l) and IAA (0.5 mg/l) + KN (1.0-5.0 mg/l) for shoots development, greater frequency (70%) were produced at IAA (0.5 mg/l) + BAP (3.0 mg/l) whereas IAA + KN were greater frequency (67%) at 3.0 mg/l KN. For root induction, four concentration of NAA were used. The maximum frequency of root formation (83%) was achieved within 3 week when isolated in vitro raised shoots were cultured on MS medium containing 0.5mg/l NAA.


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