International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 4 2 2015 February 10 Simple and Efficient Protocol for RNA and DNA extraction from Rice (Oryza sativa L.) for Downstream Applications 62 67 EN AziziFahliani Mohammad 1 Master of Science in Social Work, Razi Psychiatric Hospital, Tehran, IRAN Masafi Saideh 2 Department of Psychology, Kish International Branch, Islamic Azad University, Kish, IRAN KhankehHamid Reza 3 Department of Nursing Karolinska InstituteUniversity of Social Welfare and Rehabilitation Sciences Tehran, IRAN RahebGhonche 4 Department of Social Work University of Social Welfare and Rehabilitation Sciences, Tehran, IRAN Rezaei Omid 5 Dept. of Psychiatry, University of Social Welfare and Rehabilitation Sciences, Tehran, IRAN Sookchaloem Duangchai 1 3 Department of Forest Biology, Faculty of Forestry, Kasetsart University, Bangkok, THAILAND Methula ThulaniSihle 2 Graduate School, Kasetsart University, Bangkok, THAILAND Naris Bhumpakphan 3 Department of National Parks, Wildlife and Plant Conservation, Bangkok, THAILAND Maneerat Sompoch 4 Ajayan K.V. 1 Department of P.G Studies and Research in Botany, Karnataka State Women’s University, Bijapur, 586108, Karnataka, INDIA Babu R.L. 2 Department of P.G Studies and Research in Bioinformatics and Biotechnology, Karnataka State Women’s University, Bijapur, 586108, Karnataka, INDIA PatilBayakka B 3 KumarNayak Sandeep 1 P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, 751004, Odisha, INDIA SatapathyKunja Bihari 2 rao V.Vasudeva 1 All India Network Project on Agricultural Ornithology, Prof. Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 30. INDIA Naresh B. 2 G. Surender 3 Swamy K. 4 Farid F.B 1 Department of Zoology, University of Dhaka, Dhaka 1000, BANGLADESH Latifa G.A 2 Department of Fisheries Technology, Bangladesh Agricultural University, Mymensingh, BANGLADESH S.C.NahidM.N Chakraborty 3 Institute of Food Science and Technology, BCSIR, Dhaka 1205, BANGLADESH Begum M 4 Nath SoorajS. 1 SIAS-Centre for Scientific Research (CSR), Safi Institute of Advanced Study, Vazhayoor, Kerala, INDIA Smitha K.V. 2 Ahirwar K.R. 1 Department of Botany, Govt. P. G. College Niwari, Distt. Tikamgarh, M. P. INDIA Massar B 1 Department of Zoology, St. Anthony’s College, Shillong-793001, Meghalaya, INDIA Srinivas Leela 1 Adichunchanagiri Bitotechnology and Cancer Research Institute, B.G.Nagara-571448, Mandya, Karnataka, INDIA ThammannaGowda S.S. 2 JhalaVibhuti M 1 Centre for Advanced Studies in Plant Biotechnology and Genetic Engineering, Department of Biosciences, Saurashtra University, Rajkot, Gujarat, MandaliyaViralkumar B 2 ThakerVrinda S 3 > Research Paper 2014 12 19 2015 February 10 Plant DNA and RNA extraction is difficult due to the presence of metabolites that interfere with isolation procedures and downstream applications. In the present study DNA and RNA extraction protocol standardized from rice (Oryza sativa L.). Isolation of DNA using Triton-X-100 based extraction method with PVPP treatment efficiently removes metabolites and yield with high quality DNA from rice coleoptiles.RNA isolation methods based on TRIzol was suitable for rice leaf whereas in seed, TrisHCl (pH 9.0) with mercaptoethanol protocol gave best results. The isolated DNA and RNA proved to be suitable for PCR and RT-PCR amplification, respectively. The technique is reproducible and can be applied for PCR based markers identification and gene expression studies. Copyright@ International Science Community Association