International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Effects of Antioxidant food Supplement on aging in Bombyx Mori

Author Affiliations

  • 1Dept. of Zoology, Calicut University P.O, University of Calicut, Malappuram, Kerala, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (4), Pages 61-65, April,10 (2014)


Aging is the accumulation of deleterious changes occurring in an organism over time. It causes as a result of free radical deposition in the body which is fought out by the antioxidants. Present study mainly focuses on the dietary supplementation of antioxidants, ascorbic acid and tyrosine in the aging of Bombyx mori larvae. Total protein, hydrogen peroxide and catalase activity of haemolymph and fat body have been estimated at time interval of 24 hrs for a period of 4 days. The results obtained were statistically analysed. It was found that ascorbic acid and tyrosine acted as a potential dietary antioxidant supplement which caused significant changes in the organism’s body, by scavenging the free radicals formed in the body as a result of metabolic reactions.


  1. Devi M. and Latha P., Hepatoprotective activity of methanolic extract of Decalepis hamiltonii against acetaminophen-induced hepatic injury in rats, Int. J. Pharm. Pharmaceut. Sci.,, 400-406 (2012b)
  2. Youdin K.A. and Joseph J.A., A possible emerging phytochemicals in improving age-related neurodysfunctions, A multiplicity of effects, Free Radic. Biol.,30, 58 (2001)
  3. Nabizadeh P. and Kumar T.S.J., Fat body catalase activity as a biochemical index for the recognition of thermotolerant breeds of mulberry silkworm, Bombyx mori L, J. Therm. Biol.,36, 1-6 (2011)
  4. Keeley L.L., Physiology and biochemistry of the fat body, Comp. Insect Physiol. Biochem. Pharmacol.,, 211–248 (1985)
  5. Orr W.C. and Sohal R.S., The effects of catalase gene over expression on life span and resistance to oxidative stress in transgenic Drosophila melanogaster, Arch. Biochem, Biophys.,297, 35-41 (1992)
  6. Sohal R.S., Aging, cytochrome oxidase activity and Hrelease by mitochondria, Free Radic. Biol. Med.,14, 583-588 (1993)
  7. Hady Maha M., Potentiality of Vitamin C and / or Lupinus termis to Modulate Blood Glucose level and Oxidative Stress Status of Alloxan-induced Diabetic Rats, Int. Res. J. Biological Sci.,2(11), 41-46 (2013)
  8. Hounkpatin A.S.Y., Johnson R.C., Guédénon P., Domingo E., Alimba C.G., Boko M. and Edorh P.A., Protective Effects of Vitamin C on Haematological parameters in Intoxicated Wistar Rats with Cadmium, Mercury and Combined Cadmium and Mercury, Int. Res. J. Biological Sci.,1(8), 76-81 (2012)
  9. Javed H. and Gondal M.H., Effect of food supplementation by n and ascorbic acid on larval mortality of silkworm Bombyxmori L.), Asian J. Plant Sci.,, 556-557 (2002)
  10. Kanafi R.R., Ebadi R., Mirhosseini S.Z., Seidavi A.R., Zolfaghari M. and Etebari K.A., Review on nutritive effect of mulberry leaves enrichment with vitamins on economic traits and biological parameters of silkworm Bombyxmori L, Inv. Sur. J.,, 86-91 (2007)
  11. Kanafi R.R., Ebadi R., Fazilati M. and Mirhoseini S.Z., Nutritive effects of mulberry leaves enrichment with riboflavin vitamin on bio-economic characters of silkworm, Bombyxmori L, Arab Cong. of Plant Protec., 9, 19-23 (2006)
  12. Ito T. and Arai N., Nutrition of the silkworm, Bombyxmori. IX. Further studies in the nutritive effects of ascorbic acid, Bull. Seric. Exp. Stn., Tokyo,20, 1-19 (1965)
  13. Kaur S. and Srivastava B.G., Effect of ascorbic acid and its different quantities on various parameters of reproductive potential of Dacusccurbittae (Conquillett), Ind. J. Entomol.,57, 151-156 (1995)
  14. Amardev S., Farooq A. and Ahmed B.S., Spring rearing performance by feeding temperate mulberry variety on bivoltine hybrid NB4D2 × SH6 of silkworm, Bombyx mori L, ISCA J. Biological Sci.,1(2), 69-72 (2012)
  15. Susheela P. and Radha R., Studies on the Levels of Cholesterol and Phospholipids in the Haemolymph of the Lepidopteran Larvae before and after Stinging by the Potter Wasp, Eumenes Conica, Int. Res. J. Biological Sci.,2(3),11-15 (2013)
  16. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurement with the Folin phenol reagent, J. Biol. Chem.,193, 265– 276 (1951)
  17. 65 17.Muhlebach J., Muller K. and Schwarzenbach G., The peroxo complexes of titanium, Inorg. Chem.,11, 2381–2390 (1970)
  18. Prasad S.S. and Mohan P.M., Aminoacids, aminotransferases and proteins in the metamorphosing silkworm Bombyx mori L, Proc. Indian Acad. Sci. (Anim. Sci.),99(5), 369- 375 (1990)
  19. Shiva Kumar G. and Shamitha G., Studies on the Proteins and Proteolytic activity of indoor reared Tasar silkworm, Antheraea mylitta. D (Daba TV), Int. Res. J. Biological Sci.,2(5), 24-29 (2013)
  20. Kinnear J.F. and Thomson J.A., Nature, origin and fate of major haemolymph proteins in Calliphora, In: Insect Biochem.5, Pergamon Press Great Britain, 531-532 (1975)
  21. Kilby B.A. The biochemistry of the insect fat body, Adv. Insect Physiol.,1, 111-174 (1963)
  22. Chipppendale G.M. and Kilby B.A., Relationship between the proteins of the haemolymph and fat body during development of Pierisbrassicae, J. Insect Physiol.,15, 905-926 (1969)
  23. Martin M.D., Kinnear J.F. and Thomson J.A., Developmental changes in the late larva of Calliphorastygia. IV, Uptake of plasma protein by the fat body, Aust. J. BioI. Sci.,24, 291-299 (1971)
  24. Bandyopadhyay U., Das O. and Banerjee R.K., Reactive oxygen species. Oxidative damage and pathogenesis, Curr. Sci.,77, 658-666 (1999)