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Biodegradation of Textile Azo Dyes by Bacteria Isolated from Dyeing Industry Effluent

Author Affiliations

  • 1 Microbiology Research Laboratory, Department of Microbiology, University of Chittagong, Chittagong – 4331, BANGLADESH

Int. Res. J. Biological Sci., Volume 2, Issue (8), Pages 27-31, August,10 (2013)


Water pollution caused by industrial effluent discharges has become an alarming trend worldwide, while textile industries are considered as the most polluting among all others. In recent years, bio-treatment took attraction in removing the unwanted colour and toxicity of textile effluents than other conventional treatment processes. The present study concentrates in the isolation and identification of indigenous bacteria from textile dye effluent and evaluation of their ability to decolourize dyes. The decolourizing activity was measured spectrophotometrically after incubation of the isolates for 3, 5 and 7 days in mineral salt medium modified with 0.05% of respective Novacron dye, viz orange W3R, red FNR, yellow FN2R, blue FNR or navy WB. Three bacterial isolates exhibiting strong decolourizing activity were identified up to species as Micrococcus luteus, Listeria denitrificans and Nocardia atlantica. All the bacteria exhibited maximum decolourizing activity after 7 days of incubation with little deviation. The bacterium Micrococcus luteus caused 60% decolourization of yellow FN2R and navy WB, and 85-90% of orange W3R, red FNR and blue FNR. Likewise, Listeria denitrificans decolourized 70-80% of Blue FNR, Orange W3R, Red FNR and Navy WB. In contrast, the bacterium caused no significant decolourization of yellow FN2R. Notably, Nocardia atlantica caused almost complete decolourization of Blue FNR and Red FNR, while at least 80% of other dyes tested. This study thus reveals that some bacteria inhabit in textile effluent whereby utilize the dyes as their source of energy and nutrition, and imply their importance in treatment of industrial effluents.


  1. World Health Organization. Rapid assessment of sources of air, water and land pollution, offset publication, 62, 7 (1982)
  2. Srivastava K.P. and Singh V. K., Impact of Air-Pollution on pH of soil of Saran, Bihar, India, Res. J.Recent Sci., 1(4), 9 -13 (2012)
  3. Parikh A.N. and Mankodi P.C., Limnology of Sama Pond, Vadodara City, Gujarat, Res. J. Recent Sci., 1(1), 16 - 21 (2012)
  4. Patil S.G., Chonde S.G., Jadhav A.S. and Raut P.D., Impact of Physico chemical characteristics of Shivaji University lakes on Phytoplankton communities, Kolhapur, India, Res. J. Recent Sci., 1(2), 56 - 60 (2012)
  5. Cripps C, Bumpus A.J. and Aust S. D., Biodegradation of azo and heterocyclic dyes by Phanerochaete chrososporium, Applied Environ. Microbiol,56, 1114 – 1118 (1990)
  6. Moreira T. A., Viacava C. and Gladys V., Fed-batch Decolourization of Poly R-478 by Trametes versicolor, Braz. Arch. Biol. Technol., 47, 2 (2004)
  7. Carliell C. M., Barclay S. J., Naidoo N., Buckley C.A, Mulhol-land D. A. and Senior E., Water SA,21, 61 (1995)
  8. Metcalf and Eddy, Wastewater Engineering: Treatment, Disposal and Reuse., Mc.Graw Hill Publishing Company, Mc.Graw Hill International editions, Civil Engineering series, Singapore, 3rd edition, (1991)
  9. Spadaro J. T., Gold M.H. and Ranganathan V., Degradation of azo dyes by lignin degrading fungus Phanerochaete chrysosporium., Applied Environ. Microbiol, 58, 2397 – 2401 (1992)
  10. Saranaik S. and Kanekar P., Bioremediation of colour of methyl violet and phenol from a dye industry waste effluent using Pseudomonas sp. Isolated from factory soil, J. Applied Bacteriol, 79, 459 – 469 (1995)
  11. Lin S. H. and Peng C. F., Treatment of textile wastewater by electrochemical methods, Water Res, 28, 277–282 (1994)
  12. Karapinar K. I., Karagi F., Mcmullan G. and Marchan R., Decolourization of textile dyestuffs by a mixed bacterial consortium, Biotechnol Lett,22, 1179 –1189 (2000)
  13. McMullan G., Meehan C., Conneely A., Kirby N., Robinson T., Nigam P., Banat I. M., Marchant R. and Symth W. F., Microbial decolourization and degradation of textile dyes, Appl Microbiol Biotechnol, 56, 81 (2001)
  14. Salar R. K., Rohilla S. K. and Rohilla J. K., Decolorization of Reactive Black HFGR by Aspergillus sulphureus, Res. J. Recent Sci., 1(1), 55 - 61 (2012)
  15. Telke A. A., Kalyani D. C., Jadhav J. P. and Govindwar S. P., Kinetics and mechanism of reactive red 141 degradation by a bacterial isolate Rhizobium radiobacter MTCC 8161, Acta Chimica Slovenica,55, 320–329 (2008)
  16. Mendes S., Pereira L., Batista C. and Martins L.O., Molecular determinants of azo reduction activity in the strain Pseudomonas putida MET94, Applied Microbiology and Biotechnology,92(2), 393-405 (2011a)
  17. Feng J., Cerniglia C. E. and Chen H., Toxicological significance of azo dye metabolism by human intestinal microbiota, Front Bioscience. (Elite Ed), 1(4) 568-586 (2012)
  18. Stolz A., Basic and applied aspects in the microbial degradation of azo dyes, Applied Microbiology and Biotechnology, 56(1-2), 69-80 (2001)
  19. Walker R., The metabolism of azo compounds: a review of the literature, Food Cosmet. Toxicol, 8, 659–676 (1970)
  20. Zimmermann T., Gasser F., Kulla H. G. and Leisinger T., Comparisons of two bacterial azoreductases acquired during adaptation to growth on azo dyes, Arch. Microbiol, 138, 37 – 43 (1984)
  21. Pasti-Grigsby M. B., Paszczynski A., Goszczynski S., Crawford D. L. and Crawford R. L., Influence of aromatic substitution patterns on azo dye degradability by Streptomyces sp. and Phanerochaete chrysosporium.,Appl. Environ Microbiol, 58(11), 3605 – 3613 (1992)
  22. Buchanan R. E. and Gibbons N. E., Bergey’s Manual of Determinative Bacteriology, 8th edition, The Williams and Wilkins Company, Baltimore, (1974)
  23. Mihir L. S., Mahbubar R. K. and Farida I., Bacteria associated with textile dyeing industrial effluents and their depolarization potentiality, Bangladesh J. Microbiol, 23(1),52-54 (2006)
  24. Olukanni O. D., Osuntoki A. A. and Gbenle G. O., Decolourization of azo dyes by a strain of Micrococcusisolated from a refuse dump soil, Biotechnology, 8, 442 – 448 (2009)
  25. Φzturk A. and Abdullah M. I., Toxicological effect of indole and its azo dye derivatives on some microorganisms under aerobic conditions, Science of the total environment, 358 (1-3), 137 – 142 (2006)
  26. Sugiura W., Miyashita T., Yokoyama T. and Arai M., Isolation of azo dye degrading microorganisms and their application to white discharge printing of fabric, J. Biosci. Bioeng, 88(5), 577 – 581(1999)
  27. Pourbabaee A. A., Malekzadeh F., Sarbolouki M. N. and Najafi F., Aerobic Decolourization and Detoxification of a Disperse Dye in Textile Effluent by a New Isolate of Bacillus sp., 631– 635 Available in: (2005)
  28. Imen K., Benoit M. and Raja B. A., Decolourization of the reconstituted dye bath effluent by commercial laccase treatment: Optimization through response surface methodology, Chem. Eng. J. 156 (1): 121 – 133 (2010)