International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Molecular Characterization of Pleurotus ostreatus PO-3 involved in Mycoremediation of Benzo[a]Pyrene

    , , ,

Author Affiliations

  • 1Dept. of Microbiology, Karpagam University, Coimbatore-641021, Tamil Nadu, INDIA
  • 2 Dept. of Microbiology, Genohelix Biolabs, CASB, Jain University, Bangalore-560019, Karnataka, INDIA
  • 3 Dept. of Microbial Biotechnology, Bharathiar University, Coimbatore-641046, Tamil Nadu, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (8), Pages 48-53, August,10 (2013)

Abstract

Benzo[a]pyrene is a recalcitrant organic pollutant mainly because of the low water solubility which makes it unavailable for microbial degradation. Besides being ubiquitous in the environment, it is considered as a priority pollutant because of its carcinogenic, teratogenic and mutagenic effects. White-rot fungi such as Pleurotus ostreatus had been the organism of choice for the degradation of benzo[a]pyrene. The present study aims at the molecular identification of the selected fungal isolate by partial 18S rDNA sequencing and comparative evaluation of the benzo[a]pyrene biodegradation potential of both wild isolate and reference strain of Pleurotus ostreatus MTCC 142.Results indicated that the fungal isolate PO-3 was closely related to Pleurotus ostreatus strain Po-13 with 99% sequence similarity. The 703 bp 18S rDNA nucleotide sequence was provided a GenBank accession number KC986398. In terms of degradation potential, PO-3 isolate was a better degrader of 1g/ml of benzo[a]pyrene. Level of degradation was 32% and 29% respectively for Pleurotus ostreatus isolate PO-3 and Pleurotus ostreatus MTCC 142. Thus based on the present finding, Pleurotus ostreatus PO-3 may prove to be a promising isolate for degradation of benzo[a]pyrene.

References

  1. Paszczynski A. and Crawford R.L.,Recent advances in the use of fungi in environmental remediation and biotechnology, Soil Biochem.,10, 379-422 (2000)
  2. Rubailo A.I. and Oberenko A.V., Polycyclic aromatic hydrocarbons as priority pollutants, J. Sib. Fed. Univ., 1(4), 344-354 (2008)
  3. Juhasz A.L. and Naidu R., Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo[a]pyrene,Int. Biodeterior. Biodegrad., 45(1), 57-88 (2000)
  4. Pozdnyakova N.N.,Involvement of the ligninolytic system of white-rot and litter-decomposing fungi in the degradation of polycyclic aromatic hydrocarbons, Biotechnol. Res. Int.,(2012) , 1-20 (2012)
  5. Bogan B.W., Lamar R.T., Burgos W.D. and Tien M., Extent of humification of anthracene, fluoranthene, and benzo[]pyrene by Pleurotus ostreatus during growth in PAH-contaminated soils, Lett. Appl. Microbiol.,28(4), 250-254 (1999)
  6. Canet R., Birnstingl J.G., Malcolm D.G., Lopez-Real J.M. and Beck A.J., Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by native microflora and combinations of white-rot fungi in a coal-tar contaminated soil, Biores. Technol., 76(2), 113-117 (2001)
  7. Shahriarinour M., Wahab M.N.A., Ariff A. and Mohamad R.,Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre, Biotechnology, 10(1),108-113 (2011)
  8. Chen X., Romaine C.P., Tan Q., Schlagnhaufer B., Ospina-Giraldo D.M.D., Royse J. and Huff D.R.,PCR based genotyping of epidemic and pre epidemic Trichoderma isolates associated with green mold of Agaricus bisporus, App. Environ.Microbiol.,65(6), 2674–2678 (1999)
  9. relatedness to S. himantioides, Mycol. Res.,105(4), 447-452 (2001)
  10. Bhattacharya S., Angayarkanni J., Das A. and Palaniswamy M., Mycoremediation of Benzo[]Pyrene by Pleurotus ostreatus isolated from Wayanad district in Kerala, India, Int. J. Pharm. Bio. Sci.,2(2), 84-93 (2012)
  11. Altschul S.F., Gish W., Miller W., Myers E.W and Lipman D.J., Basic local alignment search tool. J. Mol. Biol., 215, 403–410 (1990)
  12. Desper R. and Gascuel O., Theoretical foundation of the balanced minimum evolution method of phylogenetic inference and its relationship to weighted least-squares tree fitting, Mol. Biol. Evol.,21, 587-598 (2004)
  13. Capotorti G., Digianvincenzo P., Cesti P., Bernardi A. and Guglielmetti G., Pyrene and benzo(a)pyrene metabolism by an Aspergillus terreus strain isolated from a polycyclic aromatic hydrocarbons polluted soil, Biodegradation,15(2), 79-85 (2004)
  14. Peixoto R.S., Vermelho A.B. and Rosado A.S., Petroleum-Degrading Enzymes: Bioremediation and New Prospects, Enzyme Res., (2011) , 1-7 (2011)
  15. Andreoni V. and Gianfreda L., Bioremediation and monitoring of aromatic-polluted habitats,Appl. Microbiol. Biotechnol.,76, 287-308 (2007)
  16. Sasek V., Cajthaml T. and Bhatt M., Use of fungal technology in soil remediation: a case study, Water Air. Soil. Pollut. Focus., 3(3), 5-14 (2003)
  17. Gonzalez P. and Labarère J., Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6 and V9 domains, Microbiology, 146, 209–221 (2000)
  18. Neda H. and Nakai T.,Phylogenetic analysis of Pleurotusbased on data from partial sequences of 1 SSrDNA and ITS-1 regions.In: Elliott T.J. and Balkema A.A. (eds), Science and Cultivation of Edible Fungi, Rotterdam, 161-168 (1995)
  19. Shuttleworth K.L. and Cerniglia E., Environmental aspects of PAH biodegradation, Appl. Biochem. Biotechnol.,54(1-3)291-302 (1995)
  20. Hatakka A., Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiol. Rev.,13, 125–135 (1994)
  21. Patel H., Gupte A. and Gupte S., Effect of different culture conditions and inducers on production of laccase by a basidiomycete fungal isolate Pleurotus ostreatus HP-1 under solid state fermentation, Biores., 4(1), 268-284 (2009)
  22. Hadibarata T. and Kristanti R.A., Identification of metabolites from benzo[a]pyrene oxidation by ligninolytic enzymes of Polyporus sp. S133, J. Environ. Manage., 111, 115-119 (2012)