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The Effect of Di-Ethylstilbestriol (DES), Oxytocin and Testosterone on the Content of Carbohydrate, Chlorophyll and Protein in Green Algae

Author Affiliations

  • 1Department of Microbiology, M.D.S. University, Ajmer, Rajasthan, INDIA
  • 2 Department of Zoology, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (1), Pages 35-40, January,10 (2013)


The main aim of this study was to check the influence of sex steroids: Testosterone, DiOxytocin on changes of proteins, chlorophyll and carbohydrate contents of in BG-11 medium for 15-days period, in 4 different concentrations (1 g/ml, 2.5 g/ml,steroids. It has been ascertained that the best possible stimulation of protein was caused by Di(DES) in the range from 148.55%, testosterone 64.59% and the effect of oxytocin algae was negative (decreased up to 13.33% )in comparison to a 100% control. Carbohydrate content was best encouraged by testosterone in range from 42.62% in comparison to oxytocin (15.94%) whicarbohydrate content of green algae was negative (decreased up to 26.70%). Chlorophyll content was stimulated most by testosterone in range from 94.09%, followed by oxytocin 56.69% and DES 34.39% icontrol during the whole period of 15 days of green algae cultivation. The effect on Protein, Carbohydrate aChlorophyll by Testosterone (p0.05, F(p0.05, F3.88= 491.5, r=0.9879) is statistically significant.


  1. Shekhawat D.S., Bhatnagar A., Bhatnagar M. and Panwar J., Potential of Treated Dairy Waste Water for the Cultivation of Algae and Waste Water Treatment by Algae, Universal Journal of Environmental Research and Technology, 2(1), 101-104 (2012)
  2. Szamrej I.K. and Czerpak R., The Effect of Sex Steroids and Corticosteroids on the Content of Soluble Proteins, Nucleic Acids and Reducing Sugars in Wolffia arrhiza (L.) Wimm. (Lemnaceae), Polish Journal of Environmental Studies, 13(5), 565-571(2004)
  3. Bajguz A. and Czerpak R., Metabolic activity of estradiol in Chlorella vulgaris Beijerinck (Chlorophyceae) Part II. Content of the cellular sugar and protein accumulation, Pol. Arch. Hydrobiol, 43, 427 (1996)
  4. Czerpak R., The occurence and biological activity of animal hormones and selected compounds in plants, Kosmos., 42, 613 (1993)
  5. Geuns J.M.C., Structural hormones and plant growth and development, Phytochemistry., 17, 1 (1978)
  6. Gross D. and Parthier B., Novel natural substances acting in plant growth regulation, J. Plant Growth Regul., 13, 93 (1994)
  7. Heftmann E., Steroid hormones in plants, Lloydia, 28,285 (1975)
  8. Hewitt S., Hillman J.R. and Knights B.A., Steroidal estrogens and plant growth and development, New Phytol., 85, 329 (1980)
  9. Janik J.R. and Adler J.H., Estrogen receptors in Gladiolus ovulus, Plant Physiol., 75, 135 (1984)
  10. Jones J.L. and Roddick J.G., Steroidal estrogens and androgens in relation to reproductive development in higher plant, J. Plant Physiol., 133 510 (1988)
  11. Kopcewicz J. and Rogoziska J.H., Effect of estrogens and gibberelic acid on cytokinin and abscisic acid like compound contents in pea, Experientia.,28, 1516 (1972)
  12. Kopcewicz J., Effect of estrone on the content of endogenous gibberellins in the dwarf pea, Naturwissenschaften., 56, 334 (1969)
  13. Kopcewicz J., Influence of estrogens on the auxins content in plants, Naturwissenschaften., 57, 48 (1970)
  14. Rousseau G.G., Higgins S.J., Baxter J.D., Gelfand E. and Tomkihns G.M., Binding of glucocorticoid receptors to DNA, J. Biol. Chem., 250, 6015 (1975)
  15. Geuns J.M.C., Physiological activity of corticosteroids in etiolated mung bean plants, Z. Pflanzenphysiol., 74, 2-51 (1974)
  16. Geuns J.M.C., Structure requirements of corticosteroids for physiological activity in etiolated mung bean seedlings, Z. Pflamenphysiol., 81 1 (1977)
  17. Hodge J.E. and Hofreiter B.T., Samogyi micro copper method, In Methods in Carbohydrate Chemistry, (Eds. R.L. Whistler, M.L. Wolfram) Acad. Press, New York, (1962)
  18. Slama K., Animal hormones and antihormones in plants, Biochem. Physiol. Pflanzen., 175, 177 (1980)