International Science Community Association International Research Journal of Biological Sciences 15 3 2026 08 10 A study on chitinase of Cytobacillus firmus and its applied potential 28 31 EN Auddya Deblina 1 Department of Botany, Raghunathpur College, Raghunathpur, Purulia, WB-723133, India and Department of Botany, Sidho Kanho Birsha University, Purulia, WB -723104, India Sinha Anjan Kumar 2 Department of Botany, Raghunathpur College, Raghunathpur, Purulia, WB-723133, India Puttaswamy Rajeshwari 1 Department of Botany Government Science College, Chitradurga, Karnataka, India Shukla Akshay Kumar 1 Department of Biological Sciences, Faculty of Science and Environment, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, India Saeed Mohd 2 Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia Dwivedi Vivek Dhar 3 Bioinformatics Research Division, Quanta Calculus, Greater Noida, UP, India Tripathi Ramesh Chandra 4 Department of Biological Sciences, Faculty of Science and Environment, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, India Ambekar Monal 1 Department of PG studies in Biotechnology, Nrupathunga University, NT road, Bangalore, India Khabade Sarina. P. 2 Department of PG studies in Biotechnology, Nrupathunga University, NT road, Bangalore, India > Short Communication 2024 8 22 2026 08 10 Aim of this study deals with the isolation of chitinase enzyme from cockroach carcasses and to analyze its potential biocontrol activity. The sample of cockroach carcasses were collected aseptically and subjected to serial dilution to isolate the bacterial strain. Two microorganisms were isolated and were screened for lytic enzyme in colloidal chitin media. The biochemical tests were performed to prove their validity. Further sequencing was performed to determine the species. The isolates were screened based on the clear zone formed. These isolates were subjected to biochemical test and 16s ribosomal RNA sequencing. A bacterial strain, Cytobacillus firmus, gram-negative, non-motile organism was identified. The isolates analyzed for antifungal activity showed significant percentage of inhibition. Copyright@ International Science Community Association