International Science Community Association International Research Journal of Biological Sciences 2278-3202 10 3 2021 08 10 Standardized protocol for the isolation of DNA from human saliva- a pilot study 40 44 EN . Asrinu 1 Department of Zoology, U.C.S., O.U., Hyderabad, Telangana, India Dave Urvi 1 V.P. & R.P.T.P. Science College, Vallabh Vidyanagar, Anand, Gujarat, India Bhatt Nikunj 2 V.P. & R.P.T.P. Science College, Vallabh Vidyanagar, Anand, Gujarat, India Hande Gayatri D. 1 P.G. Department of Zoology, Shri Shivaji Science College, Amravati, Maharashtra, India Adhikarimayum Aisho Sharma 1 Wildlife Institute of India, Dehradun-248001, India Hazra Priya 2 Wildlife Institute of India, Dehradun-248001, India Mikhu Jonti 3 Dibang Wildlife, Nature Conservation and Ecotourism Development Society (DWNCS), Anini, Dibang Valley District, Arunachal Pradesh, India Islam Mohammad Sohidul 1 Department of Zoology, Jahangirnagar University, Savar, Dhaka, Bangladesh Haque Md. Mansurul 2 Department of Zoology, Jahangirnagar University, Savar, Dhaka, Bangladesh S.K. Dhusia 1 ASCA Govt. P.G. College Niwari, Distt. Niwari, M.P., India J.R. Ahirwar 2 ASCA Govt. P.G. College Niwari, Distt. Niwari, M.P., India A.K. Verma 3 ASCA Govt. P.G. College Niwari, Distt. Niwari, M.P., India R.K. Ahirwar 4 ASCA Govt. P.G. College Niwari, Distt. Niwari, M.P., India M. Vajagathali 1 Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India M. Sudhan 2 Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India S. Vignesh 3 Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India T.S. Jayashree 4 Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India A. Moorthi 5 Biomaterials and Regenerative Medicine Laboratory, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Tamil Nadu-603 103, India > Short Communication 2020 12 16 2021 08 10 This study aimed to standardize the methodology for the isolation of DNA from human saliva. The present study was conducted with 20 healthy control subjects with no history of smoking habits and other oral complications. The subjects were recruited from Chettinad Dental College and Research Institute (CDCRI) with the study protocol's approval from Institutional Human Ethics Committee (392/IHEC/10-17). Their age of the subjects was ranged between 30-45 years. Approximately 5 ml of human saliva was collected in a fresh tube with informed consent from healthy controls by informing the current research study by the donors. Genomic DNA was prepared from human saliva sample by ammonium acetate method. The quantification process was determined by a UV-visible spectrophotometer (UV-1800 Shimadzu spectrophotometer) to determine the purity and concentration of DNA. The average concentration of DNA from a saliva sample was found at1.48mg/ml. Copyright@ International Science Community Association