International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 2 2012 June 10 Fungal Degradation of Azo dye- Red 3BN and Optimization of Physico-Chemical Parameters 17 24 EN Muhammad Jamshed Khan 1 Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, PAKISTAN Asad Abbas 2 University of Salmanca, SPAIN Mazhar Ayaz 3 Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, PAKISTAN Naeem Muhammad 4 Institute of Applied and Pure Biology, Bahauddin Zakariya University, Multan, PAKISTAN Saleem Qadir 5 University of Agriculture, Faisalabad, PAKISTAN Muhammad Saleem Akhter 6 Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, PAKISTAN Zia Waseem 7 Livestock and Dairy department, Government of Punjab, PAKISTAN P. Muthusamy 1 Department of Biotechnology, Karunya University,Tamil Nadu, INDIA S. Murugan 2 Department of Biotechnology, Karunya University,Tamil Nadu, INDIA Smitha Manothi 3 Department of Biotechnology, Karunya University,Tamil Nadu, INDIA Nishi K. 1 Department of Biology and Environmental Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh, INDIA N.K. Gupta 2 Department of Biology and Environmental Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh, INDIA S.C. Sharma 3 Department of Biology and Environmental Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh, INDIA Kumar Praveen G.N. 1 Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA Sumangala K. Bhat 2 Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA > Research Paper 2012 5 16 2012 June 10 Decolorization of azo dye red 3BN by three fungal species Penicillium chrysogenum, Aspergillus niger and Cladosporium sp. has been analyzed using potato dextrose agar (PDA) medium containing 0.01% of Red 3 BN. Physico-chemical parameters like carbon source, nitrogen source, temperature, pH and inoculum volume are optimized for the decolorization process by changing one parameter at a time. Optimal condition for P. chrysogenum was found to be 1% maltose 1% yeast extract, pH 8, 27�C and 2% inoculums. Ideal condition for A. niger was found to be1% maltose , 1% yeast extract, pH 8, 27C and 10% inoculum and that for Cladosporium sp. was found to be1% maltose, 1% peptone, pH 6, 37C and 10% inoculum . Extent of decolorization recorded by P. chrysogenum under ideal conditions was 99.56%, A. niger was 98.64% and that by Cladosporium sp. was 98.18%. The study has confirmed the potential of the above fungi in the decolorization of azo dye Red 3BN and opened scope for future analysis of their performance in the treatment of textile effluent. Copyright@ International Science Community Association