International Science Community Association Research Journal of Forensic Sciences 14 2 2026 07 29 Protocol development and its Efficacy testing for extraction of Nickel from Milk 18 21 EN Karthik G.I. Vaishnav 1 Department of Criminology & Forensic Science, Nehru Arts and Science College, Coimbatore, India K. Dinsha 2 Department of Forensic Science, M E S Kalladi College, Mannarkadu, Palakkad, Kerala, India R. Dhanya 3 Department of Criminology & Forensic Science, Nehru Arts and Science College, Coimbatore, India Kulkani Sudhanva G. 4 Department of Criminology & Forensic Science, Nehru Arts and Science College, Coimbatore, India S.S. Nidhin 5 Department of Criminology & Forensic Science, Nehru Arts and Science College, Coimbatore, India Bose Surajit 1 Department of Oral Maxillofacial Pathology Microbiology; Forensic Odontology, Kusum Devi Sunderlal Dugar Jain Dental College & Hospital, Ram Gopal Ghosh Road, Cossipore, Kolkata, West Bengal, India Shetty Rakshith 2 Department of Oral Maxillofacial Pathology Microbiology; Forensic Odontology, Kusum Devi Sunderlal Dugar Jain Dental College & Hospital, Ram Gopal Ghosh Road, Cossipore, Kolkata, West Bengal, India Bhattachrya Maumita 3 Department of Oral Maxillofacial Pathology Microbiology; Forensic Odontology, Kusum Devi Sunderlal Dugar Jain Dental College & Hospital, Ram Gopal Ghosh Road, Cossipore, Kolkata, West Bengal, India Sen Subhalakshmi 4 Department of Oral Maxillofacial Pathology Microbiology; Forensic Odontology, Kusum Devi Sunderlal Dugar Jain Dental College & Hospital, Ram Gopal Ghosh Road, Cossipore, Kolkata, West Bengal, India Chattopadhyay Jayanta 5 Department of Oral Maxillofacial Pathology Microbiology; Forensic Odontology, Kusum Devi Sunderlal Dugar Jain Dental College & Hospital, Ram Gopal Ghosh Road, Cossipore, Kolkata, West Bengal, India Ghosh Bitan 6 Department of Oral Maxillofacial Pathology Microbiology; Forensic Odontology, Kusum Devi Sunderlal Dugar Jain Dental College & Hospital, Ram Gopal Ghosh Road, Cossipore, Kolkata, West Bengal, India Rahangdale Labhini 1 Schiio of Biosciences and Bioengineering, Division of Forensic Science, DY Patil International University, Akurdi, Pune, Maharashtra-411044, India Dharmalingam Lokashree 2 Division of Forensic Science, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu-641114, India Mishra Manish Kumar 1 Department of Forensic Science, JAIN (deemed-to-be-University), Bengaluru, Karnataka, India Y. Kavya 2 Department of Forensic Science, JAIN (deemed-to-be-University), Bengaluru, Karnataka, India > Research Paper 2026 3 9 2026 07 29 Milk, recognized as an essential source of nutrition, is increasingly subjected to examination concerning heavy metal contamination, notably Nickel (Ni), because of its deleterious toxicological effects. The objective of this research was to establish an environmentally sustainable extraction methodology for the detection of Nickel in milk utilizing Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Milk specimens fortified with specified Nickel concentrations (1–9 ppm) underwent treatment with glacial acetic acid and sodium lauryl sulphate, followed by centrifugation, filtration, and subsequent analysis. The proposed method exhibited effective recovery rates and reliable detection capabilities, particularly at elevated concentrations. This protocol presents a sustainable, economically viable alternative for the routine monitoring of food safety and the safeguarding of public health. Copyright@ International Science Community Association