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Study of bacterial mastitis in dairy cattle of Pokhara Valley, Kaski Nepal

Author Affiliations

  • 1Department of Microbiology, Prithvi Narayan Campus, Tribhuvan University, Kaski, Nepal
  • 2Department of Microbiology, Prithvi Narayan Campus, Tribhuvan University, Kaski, Nepal

Res. J. Animal, Veterinary and Fishery Sci., Volume 11, Issue (2), Pages 1-5, November,24 (2023)


Mastitis is the most common disease in dairy cattle, and it has well-documented negative impacts on animal well-being and cattle farm profitability. It has become a major cause of milk loss from dairy farms as well as farmer economic losses. The goal of this study is to identify the bacteria that cause mastitis and their antibiogram profile. Three distinct bacteria were recovered from 145 milk samples. Cows (51%), and buffalo (49%), were among them. A total of 145 milk samples were tested for California mastitis (CMT) and 76 (52.41%) were processed. Bacterial growth was identified in just 68 (89.47%) of the 76 milk samples tested. Furthermore, 75% of the 68 mastitis positive milk samples contained S. aureus, 22% S. epidermidis, and 3% Streptococcus. Amikacin was highly effective against S. epidermidis and S. aureus, with sensitivity levels of 93.33% and 90.19%, respectively. Amikacin was 100% sensitive to Streptococcus, but Enrofloxacin was 50% sensitive to Streptococcus. Staphylococcus aureus showed 3 MDR with 3.92% and 1.96%.


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