@Research Paper
<#LINE#>Enhanced production and Purification of L-asparaginase from fusarium proliferatum and its biochemical characterization<#LINE#>Rupa @Acharya,Tapaswini @Kanungo,Nibha @Gupta <#LINE#>1-11<#LINE#>1.ISCA-RJAFS-2024-012.pdf<#LINE#>Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India@Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India@Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India<#LINE#>12/11/2024<#LINE#>18/2/2025<#LINE#>Asparaginase converts L-asparagine to L-aspartic acid and has received considerable attention in the recent years for its anti-carcinogenic potential. L-asparaginase is a drug that is used to treat lymphoblastic cancer. The ability of a new strain to produce extracellular L-asparaginase was investigated in this work. The study examined the production of L-asparaginase utilizing MCDB, which had the highest L-asparaginase activity. The optimization, synthesis, purification, and characterization of the L-Asparaginase enzyme obtained from Fusarium proliferatum are the main objectives of this investigation. After the crude enzyme was removed, it was precipitated with ammonium sulphate, filtered using a Sephadex column, and then further purified using ion exchange chromatography to yield L-asparaginase. To increase L-asparaginase synthesis, several sources and quantities of nutritional factors—such as carbon, nitrogen, and amino acid inducers—as well as physical parameters—such as pH and temperature—were adapted. The study findings indicated that the most favourable conditions for enzyme production were a pH of 8.0 at 37°C. The carbon and nitrogen sources that demonstrated the highest efficacy were Lactose (0.5%) and L-asparagine (0.5%), respectively. The yield was also high in vitamin-B1 (1%), sodium Nitrate (1%), Tween-20 (1%), Aspartic acid (1%), and Ammonium flouride (1%). The production of L-asparaginase on a large scale can be achieved through continuous fermentation using the medium composition outlined in the study.<#LINE#>Manna, S., Sinha, A., Sadhukhan, R., & Chakrabarty, S. L. (1995).@Purification, characterization and antitumor activity of L-asparaginase isolated from Pseudomonas stutzeri MB-405.@Current microbiology, 30, 291-298.@Yes$Osama, S., El-Sherei, M.M., Al-Mahdy, D.A., Bishr, M., Salama, O. and Raafat, M.M. (2023).@Optimization and characterization of antileukemic l-asparaginase produced by Fusarium solani endophyte.@AMB Express.; 13(1), 96.@Yes$Nawaz, M.S., Zhang, D., Khan, A.A. and Cerniglia, C.E. (1998).@Isolation and characterization of Enterobacter cloacae capable of metabolizing asparagine.@Applied Microbiology and Biotechnology. (50), 568-572.@Yes$Heinemann, B. and Howard, A.J. (1969).@Production of tumor-inhibitory L-asparaginase by submerged growth of Serratia marcescens.@Applied microbiology, 18(4), 550-554.@Yes$Mukherjee J, Majumdar S and Scheper T. (2000).@Studies on nutritional and oxygen requirements for production of L-asparaginase by Enterobacter aerogenes.@Applied Microbiology and Biotechnology, (53),180-4.@Yes$Dhevagi, P. and Poorani, E. (2006).@Isolation and characterization of L-asparaginase from marine actinomycetes.@Indian Journal of Biotechnology, (5), 514-520.@Yes$Narayana, K.J.P., Kumar, K.G. and Vijayalakshmi, M. (2008).@L-asparaginase production by Streptomyces albidoflavus.@Indian Journal of Microbiology, (48), 331-336.@Yes$Sahu, M.K., Poorani, E., Sivakumar, K., Thangaradjou, T. and Kannan, L. (2007).@Partial purification and anti-leukemic activity of L-asparaginase enzyme of the actinomycete strain LA-29 isolated from the estuarine fish, Mugil cephalus (Linn.).@Journal of Environmental Biology, 28(3), 645.@Yes$Verma, N., Kumar, K., Kaur, G. and Anand, S. (2007).@L-asparaginase: a promising chemotherapeutic agent.@Critical reviews in biotechnology, 27(1), 45-62.@Yes$Aghaiypour, K., Wlodawer, A. and Lubkowski, J. (2001).@Structural basis for the activity and substrate specificity of Erwinia chrysanthemi L-asparaginase.@Biochemistry, 40(19), 5655-5664.@Yes$Krasotkina, J., Borisova, A.A., Gervaziev, Y.V. and Sokolov, N. N. (2004).@One‐step purification and kinetic properties of the recombinant l‐asparaginase from Erwinia carotovora.@Biotechnology and Applied Biochemistry, 39(2), 215-221.@Yes$Keating, M.J., Holmes, R., Lerner, S. and Ho, D.H. (1993).@L-asparaginase and PEG asparaginase past, present, and future.@Leukemia & lymphoma, 10(sup1), 153-157.@Yes$Sarquis, M.I.D.M., Oliveira, E.M.M., Santos, A.S. and Costa, G.L.D. (2004).@Production of L-asparaginase by filamentous fungi@Memorias do Instituto Oswaldo Cruz.; 99, 489-492.@Yes$Ashok, A., Doriya, K., Rao, J.V., Qureshi, A., Tiwari, A.K. and Kumar, D.S. (2019).@Microbes producing L-asparaginase free of glutaminase and urease isolated from extreme locations of Antarctic soil and moss.@Scientific reports, 9(1), 1423.@Yes$Imada, A., Igarasi, S., Nakahama, K. and Isono, M. (1973).@Asparaginase and glutaminase activities of micro-organisms.@Microbiology, 76(1), 85-99.@Yes$Warangkar, S.C. and Khobragade, C.N. (2010).@Purification, characterization, and effect of thiol compounds on activity of the Erwinia carotovora L‐asparaginase.@Enzyme research, 165878.@No$Patro, K.R. and Gupta, N. (2012).@Extraction, purification and characterization of L-asparaginase from Penicillium sp. by submerged fermentation@Int J Biotechnol Mol Biol Res., 3(3), 30-34.@Yes$Prakasham, R.S., Rao, C.S., Rao, R.S., Lakshmi, G.S. and Sarma, P.N. (2007).@L‐asparaginase production by isolated Staphylococcus sp.–6A: design of experiment considering interaction effect for process parameter optimization.@Journal of applied microbiology, 102(5), 1382-1391.@Yes$Venil, C.K., Nanthakumar, K., Karthikeyan, K. and Lakshmanaperumalsamy, P. (2009).@Production of L-asparaginase by Serratia marcescens SB08: Optimization by response surface methodology.@Iranian Journal of Biotechnology, (7).@Yes$Akilandeswari, K., Kavitha, K. and Vijayalakshmi, M. (2012).@Production of bioactive enzyme L-asparaginase from fungal isolates of water sample through submerged fermentation.@Int J Pharm Pharm Sci, 363-366.@Yes$Gurunathan, B. and Sahadevan, R. (2011).@Design of experiments and artificial neural network linked genetic algorithm for modeling and optimization of L-asparaginase production by Aspergillus terreus MTCC 1782.@Biotechnology and bioprocess Engineering, (16), 50-58.@Yes$Kalyanasundaram, I., Nagamuthu, J., Srinivasan, B., Pachayappan, A. and Muthukumarasamy, S. (2015).@Production, purification and characterisation of extracellular L-asparaginase from salt marsh fungal endophytes.@World J Pharm Pharm Sci, 4(3), 663-677.@Yes$Gaffar, S.A. and Shethna, Y.I. (1977). Purification and some biological properties of asparaginase from Azotobactervinelandii.@Applied and Environmental Microbiology, 33(3), 508-514.@undefined@Yes$Sreenivasulu, V., Jayaveera, K.N. and Rao, P.M. (2009).@Optimization of process parameters for the production of L-asparaginase from an isolated fungus.@Research Journal of Pharmacognosy and Phytochemistry, 1(1), 30-34.@Yes$Deshpande, N., Choubey, P. and Agashe, M. (2014). Studies on Optimization of Growth Parameters for L‐Asparaginase Production by Streptomyces ginsengisoli.@The Scientific World Journal, 895167.@undefined@Yes$Ruby, E.J. and Raghunath, T.M. (2014).@A Review: Bacterial endophytes and their bioprospecting.@Journal of Pharmacy Research, 4(3),795-799.@Yes$Rajesh, M.J., Leelavathy, R., Vanapalli, V.S., Thirumurugan, G. and Rajaram, M.S. (2011).@Effect of inducers and physical parameters on the production of L-asparaginase using Aspergillusterrus.@J Bioprocess Biotech, (1), 1-6.@Yes$Moorthy, V., Ramalingam, A., Sumantha, A. and Shankaranaya, R.T. (2010).@Production, purification and characterisation of extracellular L-asparaginase from a soil isolate of Bacillus@sp.  Afr J Microbiol Res,  4(18), 1862-1867.@Yes$Rajesh, M.J., Leelavathy, R., Vanapalli, V.S., Thirumurugan, G. and Rajaram, M.S. (2011).@Effect of inducers and physical parameters on the production of L-asparaginase using Aspergillusterrus.@J Bioprocess Biotech, (1), 1-6.@Yes$Hosamani, R. and Kaliwal, B.B. (2011).@L-asparaginase an anti-tumor agent production by Fusarium equiseti using solid state fermentation.@Int J Drug Discov, 3(2), 88-99.@Yes$Roberts, J., Holcenberg, J.S. and Dolowy, W.C. (1972).@Isolation, crystallization, and properties of Achromobacteraceae glutaminase-asparaginase with antitumor activity.@Journal of Biological Chemistry, 247(1), 84-90.@Yes$Abdel-Fattah, Y.R. and Olama, Z.A. (2002).@L-asparaginase production by Pseudomonas aeruginosa in solid-state culture: evaluation and optimization of culture conditions using factorial designs.@Process Biochemistry, 38(1), 115-122.@Yes
@Short Communication
<#LINE#>Importance of Linseed Crops in Biological<#LINE#>Hasmat @Ali,Kiran @Pal,Javed Ahmad @Siddiqui,Sushil @Soni <#LINE#>12-14<#LINE#>2.ISCA-RJAFS-2025-001.pdf<#LINE#>Department of Botany, D.A.V. (P.G.) College, Kanpur, India@Department of Zoology, D.B.S. (P.G.) College, Kanpur, India@Department of Botany, D.A.V. (P.G.) College, Kanpur, India@Department of Botany, D.A.V. (P.G.) College, Kanpur, India<#LINE#>3/1/2025<#LINE#>3/3/2025<#LINE#>The current perusal entitled “Assessment of fatty acid outline and nutrition property of some flaxseed (Linum Usitatissimum L.) species proceed below” Sending loan was organize at C.S.A University from and analysis was away out in the laboratory sector of Biochemistry University Kanpur during winter crop season in the season 2020-21 and 2021-22. Ten kinds of flaxseed NL 93, RL 904, Garima, LCK 9211, ACC NO 442, LC 2023, KL31, Subhra, Kiran, Gaurav were together from Bruder sector of Genetic and plant bringing forth of this University and consumed as tentative stuff in the region path. The germs of flaxseed nation were sown in perfectly RBD formation with three replica on 2020-21 and 2021-22. The row to row and plant to plant difference was kept 10cm and 30cm serially. The germs were sown at the tariff of 30 to 40kg per hectare. All agronomic exercises were adopted to instate nice harvest. Through cutting the germ of every nation were together aside and used for analysis varied Biochemic factors. The germ of every species were land into the dust phase and picked in wind narrow container for ahead Biochemic limits such as species albuminoid acquiescent, methionine acquiescent, tryptophan acquiescent, lysine acquiescent. The origin material acquired in the praxis demonstrate the morphological another such as Biochemic content was record in transform LCK 9211 and Shubhra in both the season. In the entire albuminoid acquiescent was ranged from 21.60 to24.13% and 21.63 to 34.17%. Methionine 0.39 to 0.49 and .40 to 0.51 (g/16gN) tryptophan 0.33 to 0.44 and 0.34 to 0.45 (g/16gN) lysine 0.83 to 0.94 and 0.85 to 0.95 (g/16gN).<#LINE#>Essam, F., Ali, O.A., Shimarya, A & Shubhra, E.K. (2012).@Extraction and purification of lignan compound from flaxseed (linum Usitatissimun L).@Asian J. Plant Sci and Res, 2(3), 306-312.@Yes$Goyal, A., Sharma, V., Upadhyay, N., Gill, S & Sihag, M. (2014).@Flax and flaxseed oil: an ancient medicine and modern functional food.@Association of food Scientists and Technologies.@Yes$Horn, J.M., Jones, J.B & Blum, A.E. (1946).@Colorimetric determination of methionine in protein and foods.@J. Bio. Chem, 1(16), 313.@Yes$Felker, C., Libamuskas, C.K & Warner, G. (1978). Crop. Sci, 18(3), 489-90.@undefined@undefined@Yes$Alasohoimy, Horn, J.M., Jones, J.B & Blum, A.E. (1946).@Colorimetric determination of methionine in protein and foods.@J. Bio. Chem, 1(16), 313.@Yes$Madhusudan, K.T & Singh, N. (1985).@Isolation and characterization of major protein fraction (125) of flaxseed proteins.@J. Agric. Food Chem, 33, 673-677.@Yes$Oomah, B.D & Mazza, F. (1993).@Flaxseed proteins a review.@Food. Chem, 48, 109-114.@Yes$Singh, K.K., Mridula, D., Rehal, J & Barnwal, P. (2011). Flaxseed: A potential source of food, feed and fibre. Critical Reviews in Food Science and Nutrition, 51(3),210-222.@undefined@undefined@Yes$Chung, M., Lei, B & LI-Chan, E. (2005).@Isolation and structural characterization of the major protein fraction from Nor Man flax seed (Linum Usitatissimum).@Food Chemistry, 90, 271-279.@Yes$S.A., Sitoty, W.Z& EI-Masry, R.A. (2007).@Isolation and partial characterization of chick pes, lusine ans lentil seed proteins.@World Jour. of Agric. Sci, 3(1), 123-129.@Yes$Lowery, O.H., Rosebrough, N.J., Forr & Randal, R.J. (1951).@Protein measurement with the follin reagent.@Journal Biochemistry, 193, 265-275.@Yes$Zajac T. (2005).@Analysis of linseed (Linum Usitatissium L). Plant branching as related to variability and interdependence of traits Acta.@Agrobotanica, 57, 187-205@Yes
<#LINE#>Assessment of soil contamination in Industrial area of Kanpur, Uttar Pradesh, India<#LINE#>Kiran @Pal,Hasmat @Ali,Shubham @Singh,Madhuri @Yadav,Javed Ahmad @Siddiqui <#LINE#>15-18<#LINE#>3.ISCA-RJAFS-2025-002.pdf<#LINE#>Department of Zoology, D.B.S.(P.G.) College, Kanpur, India@Department of Botany, D.A-V. (P.G.) College, Kanpur, India@Department of Botany, D.A-V. (P.G.) College, Kanpur, India@Department of Zoology, D.B.S.(P.G.) College, Kanpur, India@Department of Botany, D.A-V. (P.G.) College, Kanpur, India<#LINE#>3/1/2025<#LINE#>4/3/2025<#LINE#>Geo-chemical studies were conducted to find out the of chemical pollution of Panki, Unnao and Jajmau industrial areas, located in Kanpur district of Uttar Pradesh due to industrial waste in soil. Unnao and Jajmau industrial area situated on the bank of river Ganga is a major center of leather processing and tannery industries. Apart from Unnao and Jajmau industrial area. Another industrial area is Panki. This area is spread over 20,000, acres. Panki is famous for the thermal power station which is one of the few thermal power units in Northern India. There are total 800 industrial areas in Panki, Unnao and Jajmau. Nine soil samples were collected from different layers of soil at a distance of about 1 kilometer around the industrial area of Panki, Unnao and Jajmau. To study the heavy metals like Pb, Ni, Cu, Zn, Hg and Ar in the soil samples around Panki, Jajmau and Unnao industrial area. This is one of the known and polluted areas and have smalland largescale manufacturing of pharmaceuticals, pesticides, chemicals, steel, leather, paint, machinery parts automobiles, plastic items and metal production has been going on here for many decades. The presence of heavy metal in soil is severe threat to the environment, crop production, human health and all other living organisms. The entry of poisonous and toxic metals in human food chain by the vegetables, crops is of great concern. Higher concentration of heavy metals in fields are often not caused by pedogenesis but by human activities, such as mining and smelting, combustion of fossil fuels, application of agrochemicals and wastewater irrigation. Indiscriminate dumping of hazardous waste in the study area could be the main cause of soil contamination which is spread by water, rain, wind and human activities.<#LINE#>Beg, K.R and Ali. (2008).@Chemical Contaminants and Toxicity of Ganga River Sediment from Up and Down Stream Area at Kanpur.@Ameri. J. Enviro. Sci., 4(4), 362-366.@Yes$Apte, A.D., Verma, S., Tare, VAnd Bose, P. (2005).@Oxidation of Cr 111 In Tannery Sludge to Cr VI: Field Observation and Theoretical Assessment.@J. of Hazar. Mater., 121(1-3), 215-222.@Yes$Hassanien, M.M., Kenawwy, I.M., El-Menshawy, A.M and EL-Asmy, A.A. (2008).@A Novel Method for Speciation of Cr III and Cr VI and Individual Determination Using Duolite 20 Modified with Active Hydrazones.@J. Hazar. Materi., 158(1), 170-176.@Yes$Nirel, P.M.V and Morel, F.M.M. (1990).@Pitfalls of Sequential Extractions.@Wat. R., 24(8), 1055-1056.@Yes$Ray, S., Khillare, P.S., Agarwal, T and Shridhar, V. (2008).@Assessment of PAHs in Soil Around the International Airport in Delhi India.@J. Hazar. Materi., 156(1-3), 9-16.@Yes$Armienta, M.A., Rodriguez, R., Ceniceros, N., Juarez, F and Cruz, O. (1996).@Distribution Origin and Fate of Chromium in Soil in Guanajuato, Mexico.@Enviro. Poll., 91(3), 391-397.@Yes$Gupta, R., Jindal, T., Khan, A.S., Srivastava, P and Kanaujia, A. (2019).@Assessment of Ground Water Quality and its Suitability for Drinking Industrial Area Jajmau, Kanpur India.@Pla. Archi., 19(1), 1569-1571.@Yes$Singh, K.P., Mohan, D., Sinha, S and Dalwani, R. (2004).@Impact Assessment of Treated / Untreated Wastewater Toxicants Discharge by Sewage Treatment Plants on Health, Agricultural and Environmental Quality is Waste Water Disposal Area.@Chemo., 55, 227-255.@Yes$Mapanda, F., Mangwayana, E.N., Nyamangara, J and Giller, K.E. (2005).@The Effect of Long-Term Irrigation Using Wastewater on Heavy Water Contents of Soils Under Vegetables in Harare, Zimbabwe.@Agri. Ecosys. Enviro., 107(2), 151-165.@Yes$Tariq, S.R., Shah, M.H., Shaheen, N., Khalique, A., Manzoor, S and Jaffar, M. (2005).@Multivariate Analysis of Selected Metals in Tannery Effluents and Related Soil.@J. Haz. Mat., 122(1-2), 17-22.@Yes$Sharma, R.K., Agrawal, M and Marshall, F.M. (2006).@Heavy Metals Contamination in Vegetables Grown in Wastewater Irrigated Areas of Varanasi, India.@Bul. Enviro. Conta. Toxico., 77(2), 311-318.@Yes$Tiwari, K.K., Singh, N.K., Patel, M.P., Tiwari, M.R and Rai, U.N. (2011).@Metal Contamination of Soil and Translocation in Vegetables Growing Under Industrial Wastewater Irrigated Agricultural Field of Vadodara, Gujarat, India.@Eco. Enviro. Safe., 74, 1670-1677.@Yes$Central pollution control board (2006).@Ministry of Environment and Forest, India Performance Status of Common Effluent Treatment Plants in India.@Control of Urban Pollution Series: CUPS, New Delhi.@Yes$Sinha, S., Gupta, A.K., Bhatt, K., Pandey, K., Rai, U.N and Singh, K.P. (2006).@Distribution of Metal in the Edible Plant Grow at Jajmau, Kanpur (India) Receiving Treated Tannery Waste Water: Relation with Physio-Chemical Properties of the Soil. Enviro.@Monit. Assess., 115(1-3), 1-22.@Yes$Sinha, S., Singh, S., and Mallick, S. (2008).@Comparative Growth Response of Two Varieties of Vigna Radiata L. Var.PDM54 and Var, NM I Grown on Different Tannery Sludge Applications: Effects of Treated Wastewater and Ground Water Used for Irrigation.@Enviro. Geochem. Healt., 30(5), 407-422.@Yes$Rawat, M., Ramanathan, A.L and Subramanian, V. (2009).@Quantification and Distribution of Heavy Metals from Small Scale Industrial Areas of Kanpur City, India.@J. Haza. Mate., 172, 1145-1149.@Yes$Gowd, S.S., Reddy, M.R and Govil, P.K. (2010).@Assessment of Heavy Metal Contamination in Soil at Jajmau (Kanpur) and Unnao Industrial Area of the Ganga Plain Uttar Pradesh, India.@J. Hazar. Materi., 174(1-3), 113-21.@Yes$Holidify (2025).@Jajmau Kanpur/History Leather How to Reach.@https://www.holidify.com.@Yes$Industry expert (2024).@Panki Site III Industrial Area Uttar Pradesh India.@https://www.industryexpert.co.in.@Yes$Govil, P.K., Reddy, G.L.N and Krishna, K. (2001).@Contamination of Soil Due to Heavy Metals in the Patancheru Industrial Development Area, Andhra Pradesh, India.@Enviro. Ear. Scie., 41(3), 461-669.@Yes$Mhatre, K., Phadke, P.R., Tanwar, S.R., Maydeo, H.M and Naik, V. (2022).@Effect of Heavy Metal Pollution on Total Microbial Count and Seed Germination Ability of Soil. Inter.@J. Advan. R. In Scie., Commu. Techn., 2(3), 223-227.@Yes$Sanghi, R and Sasi, K.S. (2001).@Analysing Pesticide Residues in Winter Vegetables from Kanpur. India.@J. Enviro. Healt. In pre., 43(4), 154-158.@Yes$Fisher, R.A. (1927).@Statistical Methods and Scientific Induction.@J. of the Roy. Stati. Soci. Seri., 17, 69-78.@Yes$Govil, P.K., Sorlie, J.E., Murthy, N and Sujatha, D. (2008).@Soil Contamination of Heavy Metals in the Ketedan Industrial Development Area, Hyderabad India.@Enviro. Monito. Assess., 140(1-3), 313-323.@Yes$Wuana, R and Okieimen, F.E. (2011).@Heavy Metals in Contaminated Soils: A Review of Sources Chemistry Risks and Best Available Strategies for Remediation.@I.S.R.N. Eco., 2090-4614.@Yes