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Optimization and Production of Cellulase from Agricultural Waste

Author Affiliations

  • 1Indira Gandhi Academy of Environmental Education, Research and Ecoplanning, Jiwaji University, Gwalior-474011, MP, INDIA
  • 2Department of Chemical Engineering, Institute of Engineering, Jiwaji University, Gwalior-474011, INDIA
  • 3Department of Botany, Government SMS Science College, Gwalior, MP, INDIA
  • 4Indira Gandhi Academy of Environmental Education, Research and Ecoplanning, Jiwaji University, Gwalior-474011, MP, INDIA

Res. J. Agriculture & Forestry Sci., Volume 1, Issue (7), Pages 18-20, August,8 (2013)


This work represents the optimization of agricultural waste the production of cellulase using filamentous fungi Myrothecium verrucaria has been adopted. Solid sate fermentation a potential technology for production of cellulase utilizing agricultural residues. All experiments were carried out in triplicate and mean values are reported. The appropriate incubation period is one of the important parameters for cellulase production in solid state fermentation and is governed by microorganism. The result shows that optimum incubation period is important factor for production of cellulase from agriculturalwaste. The maximum cellulase activity has been recorded after 3 days of fermentation.


  1. Varga E., Scengyel Z. and K. Recaey, Chemicalpretreatments of corn stover for enhancing enzymaticdigestibility, Appl. Biochem. Biotechnol., 98–100(1-9), 73–87 (2002)
  2. Pandey A., Solid‐state fermentation, BiochemicalEngineering Journal, 13(2), 81‐84 (2003)
  3. Maulin P. Shah, Reddy G.V., Banerjee R., Ravindra BabuP. and Kothari I.L., Microbial degradation of banana wasteunder solid state bioprocessing using two lignocellulolyticfungi (Phylosticta spp. MPS-001 and Aspergillusspp, MPS-002), Process Biochemistry, 40(1), 445–451 (2005)
  4. Pothiraj C., Balaji P. and Eyini M., Enhanced production ofcellulases by various fungal cultures in solid statefermentation of cassava waste, African Journal ofBiotechnology, 5 (20), (1882-1885)
  5. Kumara Suman, Shahana J. and Khan, Optimization ofmedium for cellulase production by Aspergillus niger usingwheat bran, Bionano Frontier, 5(2), 210-12 (2012)
  6. Murakami M., Kaneko Y. and Kadokawa J., Preparation ofcellulose-polymerized ionic liquid composite by in-situpolymerization of polymerizable ionic liquid in cellulosedissolvingsolution, Carbohydrate Polymers, 69(2), 378–381 (2007)
  7. Ghose T.K., Measurement of cellulose activities, Pure &Appl.Chem., 59, 257-268 (1987)
  8. Miller, Use of dinitrosalicylic acid reagent fordetermination of reducing sugars, Analytical Chemistry,31, 426–8 (1959)
  9. Mandels M., Andreotti R. and Roche C., , Measurment ofsaccharifying cellulose, Biotechnol Bioeng Symp., 6, 21-33(1976)