Impact of temperature and pH Variation on in-vitro Protocorm formation of Vanda tessellata (Roxb.) Hook. ex G.Don an endangered medicinal Orchid

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Impact of temperature and pH Variation on in-vitro Protocorm formation of Vanda tessellata (Roxb.) Hook. ex G.Don an endangered medicinal Orchid

Sachin Bindiya

Author Affiliations

¹ Department of Botany, Sarojini Naidu Govt. Girls Post Graduate (Autonomus) College Shivaji Nagar, Bhopal, MP, INDIA

Res. J. Recent Sci., Volume 4, Issue (IYSC-2015), Pages 10-13, September,2 (2015)

Abstract

The present research work is carried out to study the In-vitro protocorm formation of an endangered medicinal orchid Vanda tessellata at different temperature(15°C, 20°C, 25°C, 30°C) and pH (4.5, 5.5, 6.5,7.5) . Immature seeds obtained from green pods successfully shown protocorm formation on basal media MS without various combinations of growth hormones. Highest protocorm formation (94±0.17%) were observed at temperature 20°C and pH 5.5 , whereas the medium which is adjusted at temperature 15°C, 25°C, 30°C and pH 4.5, 6.5, 7.5 showed poor protocorm formation.

References

Singh M.K., Sherpa A.R., Hallan V and Zaidi A.A., Autralasian Plant pathology, 2, 11-13 (2007)
N.S. Chauhan, Medicinal and Aromatic Plants of Himachal Pradesh, Indus Publishing Company, New Delhi, (1999)
Fay M.F., Conservation of rare and endangered plants using in vitro methods, In vitro Cell Dev., Biol. — Plant; 28, 1–4 (1992)
Murashige I. and Skoog F., A revised medium for rapid growth and bioassys with tobacco tissue culture, Plant Physiol, 15, 473-497 (1962)
Bindiya Prakash, Shagufta Khan, Ritu Thakur Bais (2012) Effect of different media on In vitro Seed Germination and Protocorm Formation of Vanda tessellata (Roxb.) Hook. Ex. G an endangered medicinal orchid, Researcher, 4(12) (2012) http://www.sciencepub.net/researcher.
Bernard, La germination des orchidees, Compt. Rend. Acad. Sci. Paris, 137, 483-485 (1903)
Ito I., Germination of seed from immature pod and subsequent growth of seedling in Dendrobium nobile Lindl. Sci. Reports Saikyo Univ. Agr., 7, 35-42 (1955)
Arditti J., Factors affecting the germination of orchid seeds, Botanical Review, 33, 1-97 (1967)
Bindiya P., Ritu T.B., Prathibha S., Shagufta K., Effect of different pH on In vitro Seed Germination of Vanda tessellata (Roxb.) Hook.Ex.G an endangered medicinal orchid, Advances in Life Science and Technology, 8, (2013)
Guay A.T., Spark R.F., Bansal S., Cunningham G.R., Goodman N.F., Nankin H.R., Petak S.M. and Perez J.B., American Association of Clinical Endocrinologists Male Sexual Dysfunction Task, Force. Endocr Pract. Jan-Feb; 9(1), 77-95 (2003)
Ichihashi S and M. Yamashita, Studies on the media for orchid seed germination. I. The effects of balances inside each cation and anion group for the germination and seedling development of Bletilla striata seeds, J. Jap. Soc. Hort. Sci., 45, 407- 413 (1977)
Knudson C., A new nutrient solution for germination of orchid seed, Amer Orchid Soc Bull, 15, 214-217 (1946)
Mead J.W and C. Bulard, Vitamins and nitrogen requirement of orchis laxiflora Lamk, New Phytol., 83, 129-36 (1979)
Mitra, G.C, Studies on seed, shoot tip and stem disc of an orchid grown in aseptic culture, Indian J.Exp.Biol.,9, 79-85 (1971)
Pierik R.L.M., In vitro Culture of Higher Plants Martinus Higoff Publ, Netherlands, 344, (1987)
Prasad R.N. and G.C. Mitra, Nutrient requirement for germination of seed and development of protocorm and seedling of Cymbidium in aseptic culture, Indian J. Exp. Biol., 13, 123-26 (1975)
Quednow K.G., Beitrage Zue Frage der aufahme geloster Kohleenstoff Verbin dungen durch Orchideen und andere Pflanzen, Bot. Arch., 30, 51-108 (1930)
Raghvan V. and J.G. Torrey, Inorganic nitrogen nutrition of the embryos of the orchid Cattleya, Am. J. Bot., 51, 264-74 (1964) , 10-13 (2015)
Sinha S.K., Singh L.S. and Hegde S.N., In-vitro multiplication of Aerides rosea Loddiges ex. through asymbiotic seed germination, 16, 38-44 (1998)
Straus M.S. and D.M. Reisinger, Effect of NAA on seed germination, Am.Orchid Soc. Bull.,45, 722-23 (1976)
Vacin E and Went F., Some pH changes in nutrient solution, Bot Gardens Conserv. News, 11, 605-613 (1949)
Yakubu M.T, Akanji M.A and Oladiji A.T., Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials, Pharmacognosy Reviews, 1(1), 39-46 (2007)

[ref1] Singh M.K., Sherpa A.R., Hallan V and Zaidi A.A., Autralasian Plant pathology, 2, 11-13 (2007)

[ref2] N.S. Chauhan, Medicinal and Aromatic Plants of Himachal Pradesh, Indus Publishing Company, New Delhi, (1999)

[ref3] Fay M.F., Conservation of rare and endangered plants using in vitro methods, In vitro Cell Dev., Biol. — Plant; 28, 1–4 (1992)

[ref4] Murashige I. and Skoog F., A revised medium for rapid growth and bioassys with tobacco tissue culture, Plant Physiol, 15, 473-497 (1962)

[ref5] Bindiya Prakash, Shagufta Khan, Ritu Thakur Bais (2012) Effect of different media on In vitro Seed Germination and Protocorm Formation of Vanda tessellata (Roxb.) Hook. Ex. G an endangered medicinal orchid, Researcher, 4(12) (2012) http://www.sciencepub.net/researcher.

[ref6] Bernard, La germination des orchidees, Compt. Rend. Acad. Sci. Paris, 137, 483-485 (1903)

[ref7] Ito I., Germination of seed from immature pod and subsequent growth of seedling in Dendrobium nobile Lindl. Sci. Reports Saikyo Univ. Agr., 7, 35-42 (1955)

[ref8] Arditti J., Factors affecting the germination of orchid seeds, Botanical Review, 33, 1-97 (1967)

[ref9] Bindiya P., Ritu T.B., Prathibha S., Shagufta K., Effect of different pH on In vitro Seed Germination of Vanda tessellata (Roxb.) Hook.Ex.G an endangered medicinal orchid, Advances in Life Science and Technology, 8, (2013)

[ref10] Guay A.T., Spark R.F., Bansal S., Cunningham G.R., Goodman N.F., Nankin H.R., Petak S.M. and Perez J.B., American Association of Clinical Endocrinologists Male Sexual Dysfunction Task, Force. Endocr Pract. Jan-Feb; 9(1), 77-95 (2003)

[ref11] Ichihashi S and M. Yamashita, Studies on the media for orchid seed germination. I. The effects of balances inside each cation and anion group for the germination and seedling development of Bletilla striata seeds, J. Jap. Soc. Hort. Sci., 45, 407- 413 (1977)

[ref12] Knudson C., A new nutrient solution for germination of orchid seed, Amer Orchid Soc Bull, 15, 214-217 (1946)

[ref13] Mead J.W and C. Bulard, Vitamins and nitrogen requirement of orchis laxiflora Lamk, New Phytol., 83, 129-36 (1979)

[ref14] Mitra, G.C, Studies on seed, shoot tip and stem disc of an orchid grown in aseptic culture, Indian J.Exp.Biol.,9, 79-85 (1971)

[ref15] Pierik R.L.M., In vitro Culture of Higher Plants Martinus Higoff Publ, Netherlands, 344, (1987)

[ref16] Prasad R.N. and G.C. Mitra, Nutrient requirement for germination of seed and development of protocorm and seedling of Cymbidium in aseptic culture, Indian J. Exp. Biol., 13, 123-26 (1975)

[ref17] Quednow K.G., Beitrage Zue Frage der aufahme geloster Kohleenstoff Verbin dungen durch Orchideen und andere Pflanzen, Bot. Arch., 30, 51-108 (1930)

[ref18] Raghvan V. and J.G. Torrey, Inorganic nitrogen nutrition of the embryos of the orchid Cattleya, Am. J. Bot., 51, 264-74 (1964) , 10-13 (2015)

[ref19] Sinha S.K., Singh L.S. and Hegde S.N., In-vitro multiplication of Aerides rosea Loddiges ex. through asymbiotic seed germination, 16, 38-44 (1998)

[ref20] Straus M.S. and D.M. Reisinger, Effect of NAA on seed germination, Am.Orchid Soc. Bull.,45, 722-23 (1976)

[ref21] Vacin E and Went F., Some pH changes in nutrient solution, Bot Gardens Conserv. News, 11, 605-613 (1949)

[ref22] Yakubu M.T, Akanji M.A and Oladiji A.T., Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials, Pharmacognosy Reviews, 1(1), 39-46 (2007)