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Production of Organic Solvent Tolerant lipase from Bacillus SRR-11

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Author Affiliations

  • 1 Department of Microbiology, School of Sciences, Gujarat University, Ahmedabad 380009, Gujarat, INDIA

Res. J. Recent Sci., Volume 4, Issue (IYSC-2015), Pages 130-135, September,2 (2015)

Abstract

Lipases are vital industrial biocatalysts used in number of diverse fields like detergent, textile, oleo-chemical and food processing industries. They have the potential to hydrolyse triacyl glycerol to glycerol and fatty acids in aqueous condition. In non aqueous medium product recovery and substrate solubility can be increased by using different organic solvents. As we know in the presence of organic solvents almost enzymes deactivated, but if we isolate a strain which can produce an enzyme which can retain its activity in any harsh condition (in the presence of organic solvents), that enzyme could be an exceptional biocatalyst for the same. Consequently, bacterial strain was isolated and screened which could grow, multiply and produce an enzyme in solvent containing nutrient amended medium. This organism was also checked for its potential to produce lipase. The isolate Bacillus SRR-11 is a Gram positive rod, producing lipase in the medium comprised of 40% (v/v) solvents viz. benzene, methanol, butanol, acetone, isooctane, toluene and xylene. The log P value of all these solvents is less than 2.5. In spite of this lower log P value, the potential of lipase persists for 72 h in the presence of these organic solvents. As per our knowledge scanty reports in the literature so far are that of the solvents with log P values lower than 2.5. The isolate showed lipolytic activity at pH range of 6.0 to 9.0. Optimal lipase production was obtained at 35±2oC under shaking condition. Effect of different oils (ground nut oil, cotton seed oil, castor oil, sun flower oil, olive oil) on lipase production was also checked. Maximum lipase production was 32.26 U/ml with groundnut oil. Effect of different metal ions was checked with Mg2+, Mn2+, Fe3+, Ba2+, EDTA, Ca2+ enhancing the lipase activity. While Na+ and Cu2+ has negative effect.

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