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Study of Antibiotic Resistance Pattern of Escherichia coli PS 58 Isolated from Pichhola Lake of Udaipur, Rajasthan, India

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Author Affiliations

  • 1Department of Biotechnology, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA
  • 2Department of Botany, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA

Res. J. Recent Sci., Volume 4, Issue (ISC-2014), Pages 155-158, (2015)

Abstract

Escherichia coli is the major coliform faecal contaminating bacteria. In the present study an attempt has been made to isolate, identify and detect the antibiotics resistance pattern of E. coli PS 58. Strain PS 58 was isolated on nutrient agar from Pichhola lake of Udaipur and identified on the basis of its morphological and biochemical characteristics. For molecular identification Polymerase Chain Reaction was performed using 16S rRNA gene specific universal primers. 15 commonly used antibiotics i.e. gentamicin, kanamycin, polymyxin, tetracycline, erythromycin, ampicillin, penicillin, amikacin, ciprofloxacin, vancomycin, rifampicin, chloremphenicol, streptomycin, cefixime, trimethoprim were used to detect the antibiotic resistance pattern by disc diffusion method. The results revealed that the strain PS 58 was identified as E. coli (ascession no.KPO99424). It was found strongly resistant to kanamycin, ampicillin, cefixime, polymyxin, penicillin, vancomycin, rifampicin and streptomycin.

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