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Comparative study on various Methods for Field Preservation and DNA Extraction of Zoanthids

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Author Affiliations

  • 1Division of Marine Biology, Department of Zoology, Faculty of Science, The M. S. University of Baroda, Vadodara-390002, Gujarat, INDIA

Res. J. Recent Sci., Volume 4, Issue (8), Pages 67-71, August,2 (2015)

Abstract

Zoanthids, the benthic Anthozoans are found in nearly all coastal marine environments. Zoanthids have become recent interests in the field of scientific study because of the challenge they offer in their taxonomic discernment due to the morphological variability within a species. In these study different methods has been carried out for sample preservation and DNA extraction from Zoanthids. Sample collection and preservation in different fixatives is influential on the quality of DNA during the extraction procedure. The present study is an effort to examine six field fixatives and tries to establish an effective DNA extraction protocol for Anthozoans. Zoanthids were collected and preserved in fixatives like: 1) absolute ethanol, 2) 70% ethanol, 3) lyses buffer with CTAB, 4) Phosphate Buffer Saline with SDS 5) Liquid Nitrogen and 6) Dry Ice. The genomic DNA extraction was carried out using four different protocols, viz. Lyses buffer with proteinase K (LBWPK) 2) Lyses buffer with cetyl trimethyl ammonium bromide (LBWCTAB) 3) Lyses buffer with guanidine Isothiocyanate (LBWGIT) 4) TriZol. The most effective preservative and DNA extraction method, both in term of quality and quantity were confirmed by Gel Analysis and purity was checked by spectrophotometric method.

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