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Biological Control of Dengue Vector using Pseudomonas fluorescens

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Author Affiliations

  • 1Department of Biotechnology, School of Biotechnology and Health sciences, Karunya University, Coimbatore – 641114, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 344-351, (2014)

Abstract

In the present study Pseudomonas fluorescens from the rhizosphere soil was isolated using Kings B medium and biochemically characterised. Nutrient broth medium, King’s B medium, King’s B+ yeast extract medium and glucose peptone salt medium are used to maximize the production of exotoxin and check the anti-larvicidal activity using liquid formulation of the exoproteins which will be used for the control of dengue vector and there by eradication of the disease. The result observed that the net mortality for King’s B medium at protein concentration of 40µg and 80g/ ml for 48hrs supernatant is 100% and also the 24 hrs supernatant is active against Aedes aegypti at low concentration of protein(20µg/ml), while the 24hrs supernatant from glucose peptone salt medium shows 100% mortality at 80 g/ ml and Kings B yeast extract medium shows 100% mortality for 24hrs supernatant at protein concentration of 80µg/ml and at 40µg/ml and 80µg/ml for 48 hrs supernatant. This result shows that Kings B medium is very effective for the production of exotoxin against Aedes aegypti, since it shows their larvicidal activity at low concentration of protein.

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