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Gene Expression in Gastric Cancer for Singapore and UK Population: An Insilico Comparative Approach

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Author Affiliations

  • 1Department of Bioinformatics, Karunya University, Coimbatore , Tamilnadu, INDIA
  • 2Department Biotechnology, Karunya University, Coimbatore, Tamilnadu, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 295-301, (2014)

Abstract

Gastric adenocarcinoma is a common cancer of the digestive tract in worldwide. It occurs in men above the age of 40. This cancer is common in Asia, parts of South America, and eastern and central Europe. And it accounts for 800,000 deaths worldwide per year. Prognosis is poor due to the late detection at its advanced stage. Gene expression analysis was done for Singapore and UK Gastric cancer datasets using GeneSpring. Differentially expressed Gastric cancer Stage 1 genes were identified by performing a t-test with a p-value cutoff of 0.005 and fold change ≥ 2.Only the differentially expressed genes that satisfied the criteria was enriched to find the functional insight of the stage 1 genes in Gastric cancer. The enriched genes were analyzed for their gene ontology using a Cytoscape plug-in Bingo. Molecular interactions among the stage 1 genes were done using a Cytoscape plug-in MiMI. hnRNPC gene showed a good interaction with its neighbouring genes. SNP regions in hnRNPC gene were identified and two of them were found to be located in the promoter region. hnRNPC gene interacts with Grb 2 gene in the progression of Gastric cancer. Majority of SNPs are located in the promoter regions for Gastric cancers from Singapore and USA, it can be suggested as one of the prime reasons for its up regulation in Gastric cancer. Thus hnRNPC be gene can be used as a potential marker in the early diagnosis of Gastric cancer in Homo sapiens.

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