Biosulphidogenesis and Bioaccumulation of sulphate by moderately Thermophilic, Facultative anaerobic Bacteria Aeromonashydrophilaisolated from hot Water spring
Author Affiliations
- 1Department of Biotechnology, Birla College of Arts, Science and Commerce, Kalyan, Maharastra, INDIA
Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 203-208, (2014)
Abstract
A unique, facultative anaerobic, moderately thermophilic Sulphate reducing prokaryote (TSRP) was isolated from Vajreshwari and Ganeshpuri hot Springs of Thane, Maharashtra. The optimum temperature, pH and NaCl concentration for growth of this strain was found to 41°C, 6.5, and 4.5% respectively. The strain Si showed 100% reduction ofstandardsulphatein 11hrs., with no production of sulfide. This result reflects the presence of assimilatory sulfate reduction pathway type I, which reduces sulfate to sulfite and finally to sulfide that is accumulated in the cellfor cysteine biosynthesis. The analysis of the effluent collected from colour and dye industry showed high concentration of sulphate (689ppm).These effluent wassubjected to sulphidogenesis by strain Si and there was complete reduction of sulphate in 12.30 hrs.with no production of sulphide. Phylogenetic analysis of 16s rRNA sequence placed strain Si in gamma subclass of proteobacter, showing highly similarity with other phylogenetic relatives. Thus this isolate is a member of genus Aeromonas and the type strain is hydrophila strain ZHYYZ-1. The result indicated that Aeromonashydrophila strain ZHYYZ-1 has high efficiency of sulphate reduction in much less time with no production of sulfide than previously studied anaerobic bacteria.
References
- Cloetete., Gerber a. and Maritz l. V., A first order inventory of water use and effluent production by SA industrial, mining and electricity generation sectors, Arcusgibb (pty) ltd, water resource division (water – north), pretoria, wrc report no. 1547/1/10 ( 2010)
- KrishnanandanVivek, Srikantaswamy S, Assessment of impacts by Industries on sediments of Kabini river around Nanjangud Industrial area, Karnataka, India, International Journal of Scientific and Engineering Research, 4(11)(2013)
- Vemula M., Ambavaram V., Kalluru G. and Gajulapalle M., Tollamadugu N. An Overview on Research Trends in Remediation of Chromium, Research Journal of Recent Sciences, 2(1), 71-83, January (2013)
- Anna H. Kaksonen, Jason J. Plumb,Wendy J. Robertson, Stefan Spring, Peter Schumann, Peter D. Franzmann and Jaakko A. Puhakka, Novel Thermophilic Sulfate-Reducing Bacteria From A Geothermally Active Underground Mine In Japan. Appl Environ Microbiol., May, 72(5), 3759–3762 (2006)
- Carine Audiffrin, Jean-Luc Cayol, Catherine Joulian, Laurence Casalot, Pierre Thomas, Jean-Louis Garcia and Bernard OllivierDesulfonauticussubmarinus gen. nov., sp. nov., a novel sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent, International Journal Of Systematic and Evolutionary Microbiology, (5), 1585-1590 (2003)
- Francis Amala Rejula and Masilamai Dhinakaran, Removal of Zinc (II) by Non Living Biomass of Agaricus Bisporus, Research Journal of Recent Sciences, 1(9), 13-17 (2012)
- Elisa Bayraktarov, Roy E. Price, Timothy G. Ferdelman and Kai Finster, The pH and pCO dependence of sulfate reduction in shallow-sea hydrothermal CO – venting sediments (Milos Island, Greece), Front. In Micro (2013)
- Mehar Fatma, M. Iqbal R., Khan Asim Massod and nafees A. Khan, Coordinate changes in assimilatory Sulphate reduction are correlated to salt tolerance : Involvemetn of Phytohormones, Annual review and Research in biology, 3(3) (2013)
- Z. Manafi1, M. Hashemi1, H. Abdollahi,Gregory. J. Olson, Bio-corrosion of water pipeline by sulphate-reducing bacteria in a mining environment, Research Journal of Recent Sciences,12(46), 6504-6516 ( 2013)
- Murhekar Gopalkrushna Haribhau, Trace Metals Contamination of Surface Water Samples in and Around Akot City in Maharashtra, India, Research Journal of Recent Sciences, 1(7), 5-9 (2012)
