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Study on Fructosyltransferase enzyme from Aspergillus sp. in Fructooligosaccharides production

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Author Affiliations

  • 1Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 147-153, (2014)

Abstract

The fructosyltransferase intra- and extra- cellular enzyme preparations obtained from Aspergillus sp. was used to produce fructooligosaccharides by enzymatic conversion of sucrose. The crude enzyme preparation of Aspergillus sp. exhibited fructosyltransferase activity of 5.0 U.mg-1 at 60 per cent sucrose concentration. The specific activity was recorded at its highest rate at the substrate concentration of 40 per cent (9.05 U.min-1.mg-1 of protein). The high performance liquid chromatography analysis of the end products of fructosyl enzyme activity on sucrose had shown the formation of, 1-kestose and nystose along with glucose, fructose and unhydrolyzed sucrose. The enzyme activity was stable at a temperature of 52°C and at a pH of 4.5. The activity of enzyme was enhanced by addition of 1 mM FeSO (7.1 U.min-1.mg-1 of protein) and also by addition of Fe2+ and its combination with Ca2+ (9.2 U.min-1.mg-1 of protein). Susceptibility to detergents was observed. The selectivity of conversion of sucrose to fructooligosaccharides obtained by the enzyme was approximately 70 per cent under optimized conditions. The partially purified fructosyltransferase preparation from Aspergillus sp. is found applicable for industrial production of fructooligosaccharides.

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