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Quantitative and qualitative analysis of protein in haemolymph of sixth instar larvae of Orthaga exvinacea Hampson (Lepidoptera: Pyralidae) treated with leaf extracts of Clerodendrum infortunatum and Chromolaena odorata

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Author Affiliations

  • 1PG and Research Dept. of Zoology, Malabar Christian College, Calicut, Kerala-673001, India
  • 2PG and Research Dept. of Zoology, Malabar Christian College, Calicut, Kerala-673001, India

Res. J. Recent Sci., Volume 11, Issue (4), Pages 10-17, October,2 (2022)

Abstract

Orthaga exvinacea is an agriculturally important major pest of mango crop. In caterpillar stage the yield of crop is reduced as they defoliate leaves. Eradication of this pest by using synthetic insecticide is creating serious health hazards to non-target organisms and severe environmental pollution. In such a scenario, use of Botanical insecticides are effective, ecologically acceptable and safe instead of uncontrolled used of insecticide. As a result, the purpose of this study was to see how methanolic leaf extracts of Clerodendrum infortunatum and Chromolaena odorata affected protein content and protein profile in haemolymph of the sixth instar larval stage of Orthaga exvinacea under laboratory circumstances. Sixth instar larvae of the pest were fed with the 1-5 different concentrations of both leaf extracts treated mango leaves. The larvae were sacrificed after 48 hours to collect haemolymph and bioassay was carried out. The quantitative study of protein showed that there was some considerable decrease in the amount of protein with the increase in concentration of botanicals. In addition to this, electrophoretic analysis of haemolymph protein showed some alterations in protein profile of botanical treated samples compared to control. Both appearances of new protein bands and disappearances of some protein bands were noticed in protein profile of treated larvae with increase in botanical concentrations. The study discovered that C. odorata had a higher efficacy than C. infortunatum in altering the physiology of larvae, and that both botanicals have the potential to be utilized as new natural Biopesticides.

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